Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00740 and MMP_RS00745 are separated by 77 nucleotides MMP_RS00745 and MMP_RS00750 are separated by 15 nucleotides MMP_RS00750 and MMP_RS00755 are separated by 144 nucleotides
MMP_RS00740: MMP_RS00740 - cyclase family protein, at 134,996 to 135,565
_RS00740
MMP_RS00745: MMP_RS00745 - histidinol phosphate phosphatase domain-containing protein, at 135,643 to 136,299
_RS00745
MMP_RS00750: MMP_RS00750 - fumarate hydratase, at 136,315 to 137,160
_RS00750
MMP_RS00755: MMP_RS00755 - tyrosine decarboxylase MfnA, at 137,305 to 138,459
_RS00755
Position (kb) 136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 135.338 kb on + strand, within MMP_RS00740 at 135.421 kb on - strand, within MMP_RS00740 at 135.422 kb on + strand, within MMP_RS00740 at 135.565 kb on - strand at 135.600 kb on + strand at 135.606 kb on + strand at 135.613 kb on - strand at 135.613 kb on - strand at 135.619 kb on + strand at 135.675 kb on - strand at 135.800 kb on - strand at 136.395 kb on + strand at 136.454 kb on - strand, within MMP_RS00750 at 136.665 kb on - strand, within MMP_RS00750 at 136.688 kb on - strand, within MMP_RS00750 at 136.868 kb on - strand, within MMP_RS00750 at 137.061 kb on + strand, within MMP_RS00750 at 137.179 kb on + strand at 137.207 kb on - strand at 137.522 kb on - strand at 137.551 kb on + strand at 137.829 kb on - strand at 137.933 kb on - strand at 137.934 kb on + strand at 137.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 135,338 + MMP_RS00740 0.60 +0.9 135,421 - MMP_RS00740 0.75 -0.2 135,422 + MMP_RS00740 0.75 +0.3 135,565 - +0.1 135,600 + -0.6 135,606 + -0.4 135,613 - -1.6 135,613 - -1.2 135,619 + -0.5 135,675 - -3.1 135,800 - -2.1 136,395 + +0.2 136,454 - MMP_RS00750 0.16 +0.1 136,665 - MMP_RS00750 0.41 -0.4 136,688 - MMP_RS00750 0.44 -0.8 136,868 - MMP_RS00750 0.65 -1.2 137,061 + MMP_RS00750 0.88 -0.2 137,179 + -0.2 137,207 - -0.3 137,522 - -0.1 137,551 + -1.1 137,829 - -1.4 137,933 - -0.1 137,934 + -0.4 137,943 - +0.6
Or see this region's nucleotide sequence