Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02735

Experiment: D-Salicin (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02730 and BWI76_RS02735 are separated by 191 nucleotidesBWI76_RS02735 and BWI76_RS02740 overlap by 11 nucleotidesBWI76_RS02740 and BWI76_RS02745 are separated by 322 nucleotides BWI76_RS02730: BWI76_RS02730 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 552,455 to 554,398 _RS02730 BWI76_RS02735: BWI76_RS02735 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 554,590 to 555,333 _RS02735 BWI76_RS02740: BWI76_RS02740 - putative transcriptional regulator, at 555,323 to 555,880 _RS02740 BWI76_RS02745: BWI76_RS02745 - hypothetical protein, at 556,203 to 556,409 _RS02745 Position (kb) 554 555 556Strain fitness (log2 ratio) -3 -2 -1 0 1at 553.902 kb on + strand, within BWI76_RS02730at 553.991 kb on + strand, within BWI76_RS02730at 554.159 kb on + strand, within BWI76_RS02730at 554.203 kb on - strand, within BWI76_RS02730at 554.227 kb on + strandat 554.227 kb on + strandat 554.267 kb on + strandat 554.380 kb on - strandat 554.390 kb on - strandat 554.431 kb on + strandat 554.567 kb on + strandat 554.567 kb on + strandat 554.736 kb on - strand, within BWI76_RS02735at 554.803 kb on - strand, within BWI76_RS02735at 554.963 kb on - strand, within BWI76_RS02735at 555.079 kb on - strand, within BWI76_RS02735at 555.173 kb on - strand, within BWI76_RS02735at 555.207 kb on - strand, within BWI76_RS02735at 555.478 kb on - strand, within BWI76_RS02740at 555.568 kb on + strand, within BWI76_RS02740at 555.634 kb on - strand, within BWI76_RS02740at 555.683 kb on + strand, within BWI76_RS02740at 555.683 kb on + strand, within BWI76_RS02740at 555.763 kb on + strand, within BWI76_RS02740at 555.775 kb on + strand, within BWI76_RS02740at 555.798 kb on - strand, within BWI76_RS02740at 555.874 kb on + strandat 555.882 kb on - strandat 555.883 kb on - strandat 556.228 kb on - strand, within BWI76_RS02745

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Salicin (C)
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553,902 + BWI76_RS02730 0.74 +0.0
553,991 + BWI76_RS02730 0.79 +0.4
554,159 + BWI76_RS02730 0.88 -0.9
554,203 - BWI76_RS02730 0.90 -0.2
554,227 + +0.3
554,227 + -0.7
554,267 + -0.6
554,380 - -1.8
554,390 - +1.2
554,431 + -1.1
554,567 + +0.2
554,567 + -0.7
554,736 - BWI76_RS02735 0.20 -2.2
554,803 - BWI76_RS02735 0.29 -2.2
554,963 - BWI76_RS02735 0.50 +0.0
555,079 - BWI76_RS02735 0.66 -3.1
555,173 - BWI76_RS02735 0.78 -2.9
555,207 - BWI76_RS02735 0.83 -1.4
555,478 - BWI76_RS02740 0.28 +0.2
555,568 + BWI76_RS02740 0.44 +0.0
555,634 - BWI76_RS02740 0.56 -0.4
555,683 + BWI76_RS02740 0.65 +0.3
555,683 + BWI76_RS02740 0.65 +0.7
555,763 + BWI76_RS02740 0.79 -0.8
555,775 + BWI76_RS02740 0.81 +0.5
555,798 - BWI76_RS02740 0.85 -0.3
555,874 + +0.2
555,882 - -1.0
555,883 - -0.5
556,228 - BWI76_RS02745 0.12 -0.6

Or see this region's nucleotide sequence