Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00835

Experiment: D-Salicin (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00825 and BWI76_RS00830 are separated by 151 nucleotidesBWI76_RS00830 and BWI76_RS00835 are separated by 97 nucleotidesBWI76_RS00835 and BWI76_RS00840 are separated by 254 nucleotides BWI76_RS00825: BWI76_RS00825 - AraC family transcriptional regulator, at 168,919 to 169,782 _RS00825 BWI76_RS00830: BWI76_RS00830 - metal-binding protein ZinT, at 169,934 to 170,581 _RS00830 BWI76_RS00835: BWI76_RS00835 - phosphoenolpyruvate carboxylase, at 170,679 to 173,330 _RS00835 BWI76_RS00840: BWI76_RS00840 - acetylornithine deacetylase, at 173,585 to 174,736 _RS00840 Position (kb) 170 171 172 173 174Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 169.825 kb on + strandat 169.843 kb on - strandat 169.919 kb on + strandat 169.927 kb on - strandat 169.982 kb on - strandat 170.030 kb on - strand, within BWI76_RS00830at 170.082 kb on - strand, within BWI76_RS00830at 170.235 kb on - strand, within BWI76_RS00830at 170.241 kb on + strand, within BWI76_RS00830at 170.251 kb on + strand, within BWI76_RS00830at 170.552 kb on - strandat 170.664 kb on - strandat 170.694 kb on - strandat 170.785 kb on + strandat 170.917 kb on - strandat 170.939 kb on + strandat 170.950 kb on + strand, within BWI76_RS00835at 170.968 kb on - strand, within BWI76_RS00835at 171.149 kb on - strand, within BWI76_RS00835at 171.387 kb on - strand, within BWI76_RS00835at 171.426 kb on + strand, within BWI76_RS00835at 171.434 kb on - strand, within BWI76_RS00835at 171.434 kb on - strand, within BWI76_RS00835at 171.502 kb on - strand, within BWI76_RS00835at 171.652 kb on + strand, within BWI76_RS00835at 171.676 kb on + strand, within BWI76_RS00835at 171.760 kb on + strand, within BWI76_RS00835at 171.768 kb on - strand, within BWI76_RS00835at 171.960 kb on + strandat 171.979 kb on - strand, within BWI76_RS00835at 172.047 kb on + strand, within BWI76_RS00835at 172.115 kb on + strand, within BWI76_RS00835at 172.127 kb on + strand, within BWI76_RS00835at 172.244 kb on - strand, within BWI76_RS00835at 172.641 kb on + strand, within BWI76_RS00835at 172.692 kb on - strand, within BWI76_RS00835at 172.853 kb on + strand, within BWI76_RS00835at 172.853 kb on + strand, within BWI76_RS00835at 172.861 kb on - strand, within BWI76_RS00835at 172.861 kb on - strand, within BWI76_RS00835at 172.928 kb on + strand, within BWI76_RS00835at 173.110 kb on - strandat 173.127 kb on - strandat 173.311 kb on + strandat 173.334 kb on + strandat 173.342 kb on - strandat 173.539 kb on + strandat 173.619 kb on - strandat 173.698 kb on - strandat 173.737 kb on + strand, within BWI76_RS00840at 173.863 kb on + strand, within BWI76_RS00840at 173.960 kb on - strand, within BWI76_RS00840at 173.960 kb on - strand, within BWI76_RS00840at 174.257 kb on + strand, within BWI76_RS00840

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Salicin (C)
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169,825 + +0.3
169,843 - +0.9
169,919 + +0.3
169,927 - -1.0
169,982 - +0.1
170,030 - BWI76_RS00830 0.15 +0.4
170,082 - BWI76_RS00830 0.23 +0.3
170,235 - BWI76_RS00830 0.46 +0.2
170,241 + BWI76_RS00830 0.47 +0.1
170,251 + BWI76_RS00830 0.49 +0.0
170,552 - -0.2
170,664 - -1.4
170,694 - +0.6
170,785 + -2.3
170,917 - -4.4
170,939 + -3.9
170,950 + BWI76_RS00835 0.10 -4.4
170,968 - BWI76_RS00835 0.11 -3.1
171,149 - BWI76_RS00835 0.18 -2.7
171,387 - BWI76_RS00835 0.27 -3.2
171,426 + BWI76_RS00835 0.28 -3.9
171,434 - BWI76_RS00835 0.28 -4.3
171,434 - BWI76_RS00835 0.28 -2.2
171,502 - BWI76_RS00835 0.31 -3.5
171,652 + BWI76_RS00835 0.37 -3.7
171,676 + BWI76_RS00835 0.38 -5.1
171,760 + BWI76_RS00835 0.41 +0.0
171,768 - BWI76_RS00835 0.41 -3.3
171,960 + +0.3
171,979 - BWI76_RS00835 0.49 -2.3
172,047 + BWI76_RS00835 0.52 -3.4
172,115 + BWI76_RS00835 0.54 -2.2
172,127 + BWI76_RS00835 0.55 -3.4
172,244 - BWI76_RS00835 0.59 -1.2
172,641 + BWI76_RS00835 0.74 -1.0
172,692 - BWI76_RS00835 0.76 -2.1
172,853 + BWI76_RS00835 0.82 -4.2
172,853 + BWI76_RS00835 0.82 -4.5
172,861 - BWI76_RS00835 0.82 -4.5
172,861 - BWI76_RS00835 0.82 -1.2
172,928 + BWI76_RS00835 0.85 -2.5
173,110 - -4.9
173,127 - -3.4
173,311 + -1.8
173,334 + -4.2
173,342 - +1.0
173,539 + +0.3
173,619 - +0.4
173,698 - +3.0
173,737 + BWI76_RS00840 0.13 +0.0
173,863 + BWI76_RS00840 0.24 -0.4
173,960 - BWI76_RS00840 0.33 -0.6
173,960 - BWI76_RS00840 0.33 -0.5
174,257 + BWI76_RS00840 0.58 -0.1

Or see this region's nucleotide sequence