Experiment: Nickel (II) chloride 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1542 and BT1543 are separated by 565 nucleotides BT1543 and BT1544 are separated by 86 nucleotides BT1544 and BT1545 are separated by 6 nucleotides
BT1542: BT1542 - putative pyridine nucleotide-disulfide oxidoreductase (NCBI ptt file), at 1,901,116 to 1,902,489
BT1542
BT1543: BT1543 - putative ABC transporter ATP-binding protein (NCBI ptt file), at 1,903,055 to 1,904,653
BT1543
BT1544: BT1544 - NADH pyrophosphatase, MutT family hydrolase (NCBI ptt file), at 1,904,740 to 1,905,528
BT1544
BT1545: BT1545 - conserved hypothetical protein, putative regulatory protein with a cAMP-binding domain, (NCBI ptt file), at 1,905,535 to 1,906,098
BT1545
Position (kb)
1903
1904
1905 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1902.070 kb on + strand, within BT1542 at 1902.164 kb on + strand, within BT1542 at 1902.330 kb on + strand, within BT1542 at 1902.330 kb on + strand, within BT1542 at 1902.345 kb on + strand, within BT1542 at 1902.345 kb on + strand, within BT1542 at 1902.345 kb on + strand, within BT1542 at 1902.346 kb on - strand, within BT1542 at 1902.381 kb on + strand at 1902.382 kb on - strand at 1902.418 kb on - strand at 1902.418 kb on - strand at 1902.486 kb on - strand at 1902.611 kb on + strand at 1902.612 kb on - strand at 1902.617 kb on + strand at 1902.667 kb on - strand at 1902.688 kb on - strand at 1902.694 kb on - strand at 1902.694 kb on - strand at 1902.698 kb on - strand at 1902.698 kb on - strand at 1902.831 kb on - strand at 1902.845 kb on + strand at 1902.867 kb on + strand at 1902.867 kb on + strand at 1902.867 kb on + strand at 1902.868 kb on - strand at 1902.871 kb on + strand at 1902.872 kb on - strand at 1902.898 kb on - strand at 1902.912 kb on - strand at 1902.915 kb on - strand at 1902.984 kb on - strand at 1903.064 kb on - strand at 1903.064 kb on - strand at 1903.083 kb on - strand at 1903.104 kb on + strand at 1903.183 kb on + strand at 1903.256 kb on + strand, within BT1543 at 1903.280 kb on + strand, within BT1543 at 1903.314 kb on + strand, within BT1543 at 1903.321 kb on + strand, within BT1543 at 1903.343 kb on - strand, within BT1543 at 1903.343 kb on - strand, within BT1543 at 1903.460 kb on + strand, within BT1543 at 1903.513 kb on + strand, within BT1543 at 1903.513 kb on + strand, within BT1543 at 1903.539 kb on + strand, within BT1543 at 1903.592 kb on + strand, within BT1543 at 1903.671 kb on + strand, within BT1543 at 1903.701 kb on - strand, within BT1543 at 1903.703 kb on + strand, within BT1543 at 1903.704 kb on - strand, within BT1543 at 1903.809 kb on + strand, within BT1543 at 1903.893 kb on + strand, within BT1543 at 1904.267 kb on + strand, within BT1543 at 1904.272 kb on - strand, within BT1543 at 1904.330 kb on + strand, within BT1543 at 1904.367 kb on + strand, within BT1543 at 1904.391 kb on + strand, within BT1543 at 1904.482 kb on + strand, within BT1543 at 1904.483 kb on - strand, within BT1543 at 1904.483 kb on - strand, within BT1543 at 1904.501 kb on - strand at 1904.532 kb on + strand at 1904.533 kb on - strand at 1904.551 kb on + strand at 1904.579 kb on - strand at 1904.607 kb on - strand at 1904.641 kb on - strand at 1904.652 kb on - strand at 1904.686 kb on - strand at 1904.686 kb on - strand at 1904.688 kb on - strand at 1904.868 kb on + strand, within BT1544 at 1904.869 kb on - strand, within BT1544 at 1904.869 kb on - strand, within BT1544 at 1904.873 kb on + strand, within BT1544 at 1904.932 kb on - strand, within BT1544 at 1904.950 kb on + strand, within BT1544 at 1905.002 kb on - strand, within BT1544 at 1905.097 kb on - strand, within BT1544 at 1905.185 kb on + strand, within BT1544 at 1905.385 kb on + strand, within BT1544 at 1905.523 kb on - strand at 1905.647 kb on + strand, within BT1545 at 1905.647 kb on + strand, within BT1545 at 1905.647 kb on + strand, within BT1545
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.01 mM remove 1,902,070 + BT1542 0.69 -1.2 1,902,164 + BT1542 0.76 -6.0 1,902,330 + BT1542 0.88 +0.8 1,902,330 + BT1542 0.88 -0.2 1,902,345 + BT1542 0.89 +0.2 1,902,345 + BT1542 0.89 -0.7 1,902,345 + BT1542 0.89 -0.4 1,902,346 - BT1542 0.90 -0.6 1,902,381 + -0.7 1,902,382 - +0.0 1,902,418 - -0.8 1,902,418 - -1.0 1,902,486 - +0.6 1,902,611 + +0.0 1,902,612 - +0.2 1,902,617 + +0.2 1,902,667 - -2.7 1,902,688 - -0.1 1,902,694 - -1.0 1,902,694 - -1.6 1,902,698 - -0.4 1,902,698 - +0.0 1,902,831 - +0.2 1,902,845 + -1.4 1,902,867 + -0.5 1,902,867 + +0.1 1,902,867 + +0.6 1,902,868 - -1.0 1,902,871 + +0.4 1,902,872 - +1.6 1,902,898 - -0.7 1,902,912 - +0.6 1,902,915 - -1.3 1,902,984 - +0.6 1,903,064 - -1.0 1,903,064 - -1.6 1,903,083 - +2.0 1,903,104 + -1.0 1,903,183 + -0.3 1,903,256 + BT1543 0.13 -0.4 1,903,280 + BT1543 0.14 -1.8 1,903,314 + BT1543 0.16 +0.0 1,903,321 + BT1543 0.17 -1.2 1,903,343 - BT1543 0.18 -1.1 1,903,343 - BT1543 0.18 +0.0 1,903,460 + BT1543 0.25 -1.4 1,903,513 + BT1543 0.29 +0.6 1,903,513 + BT1543 0.29 -0.7 1,903,539 + BT1543 0.30 -1.0 1,903,592 + BT1543 0.34 -0.2 1,903,671 + BT1543 0.39 -1.2 1,903,701 - BT1543 0.40 -0.4 1,903,703 + BT1543 0.41 -0.7 1,903,704 - BT1543 0.41 -2.0 1,903,809 + BT1543 0.47 +0.0 1,903,893 + BT1543 0.52 -1.2 1,904,267 + BT1543 0.76 -0.8 1,904,272 - BT1543 0.76 -0.9 1,904,330 + BT1543 0.80 -1.4 1,904,367 + BT1543 0.82 +1.6 1,904,391 + BT1543 0.84 +0.6 1,904,482 + BT1543 0.89 -1.2 1,904,483 - BT1543 0.89 -2.3 1,904,483 - BT1543 0.89 -0.9 1,904,501 - -0.4 1,904,532 + -1.7 1,904,533 - -0.9 1,904,551 + -2.1 1,904,579 - -0.5 1,904,607 - -0.7 1,904,641 - -0.2 1,904,652 - -0.6 1,904,686 - -0.6 1,904,686 - -1.6 1,904,688 - -0.7 1,904,868 + BT1544 0.16 -0.4 1,904,869 - BT1544 0.16 -0.9 1,904,869 - BT1544 0.16 +0.3 1,904,873 + BT1544 0.17 +1.3 1,904,932 - BT1544 0.24 -0.7 1,904,950 + BT1544 0.27 -1.4 1,905,002 - BT1544 0.33 +2.6 1,905,097 - BT1544 0.45 -1.3 1,905,185 + BT1544 0.56 -0.4 1,905,385 + BT1544 0.82 -0.9 1,905,523 - -1.7 1,905,647 + BT1545 0.20 -0.5 1,905,647 + BT1545 0.20 +0.6 1,905,647 + BT1545 0.20 -0.4
Or see this region's nucleotide sequence