Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1543

Experiment: Nickel (II) chloride 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1542 and BT1543 are separated by 565 nucleotidesBT1543 and BT1544 are separated by 86 nucleotidesBT1544 and BT1545 are separated by 6 nucleotides BT1542: BT1542 - putative pyridine nucleotide-disulfide oxidoreductase (NCBI ptt file), at 1,901,116 to 1,902,489 BT1542 BT1543: BT1543 - putative ABC transporter ATP-binding protein (NCBI ptt file), at 1,903,055 to 1,904,653 BT1543 BT1544: BT1544 - NADH pyrophosphatase, MutT family hydrolase (NCBI ptt file), at 1,904,740 to 1,905,528 BT1544 BT1545: BT1545 - conserved hypothetical protein, putative regulatory protein with a cAMP-binding domain, (NCBI ptt file), at 1,905,535 to 1,906,098 BT1545 Position (kb) 1903 1904 1905Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 1902.070 kb on + strand, within BT1542at 1902.164 kb on + strand, within BT1542at 1902.330 kb on + strand, within BT1542at 1902.330 kb on + strand, within BT1542at 1902.345 kb on + strand, within BT1542at 1902.345 kb on + strand, within BT1542at 1902.345 kb on + strand, within BT1542at 1902.346 kb on - strand, within BT1542at 1902.381 kb on + strandat 1902.382 kb on - strandat 1902.418 kb on - strandat 1902.418 kb on - strandat 1902.486 kb on - strandat 1902.611 kb on + strandat 1902.612 kb on - strandat 1902.617 kb on + strandat 1902.667 kb on - strandat 1902.688 kb on - strandat 1902.694 kb on - strandat 1902.694 kb on - strandat 1902.698 kb on - strandat 1902.698 kb on - strandat 1902.831 kb on - strandat 1902.845 kb on + strandat 1902.867 kb on + strandat 1902.867 kb on + strandat 1902.867 kb on + strandat 1902.868 kb on - strandat 1902.871 kb on + strandat 1902.872 kb on - strandat 1902.898 kb on - strandat 1902.912 kb on - strandat 1902.915 kb on - strandat 1902.984 kb on - strandat 1903.064 kb on - strandat 1903.064 kb on - strandat 1903.083 kb on - strandat 1903.104 kb on + strandat 1903.183 kb on + strandat 1903.256 kb on + strand, within BT1543at 1903.280 kb on + strand, within BT1543at 1903.314 kb on + strand, within BT1543at 1903.321 kb on + strand, within BT1543at 1903.343 kb on - strand, within BT1543at 1903.343 kb on - strand, within BT1543at 1903.460 kb on + strand, within BT1543at 1903.513 kb on + strand, within BT1543at 1903.513 kb on + strand, within BT1543at 1903.539 kb on + strand, within BT1543at 1903.592 kb on + strand, within BT1543at 1903.671 kb on + strand, within BT1543at 1903.701 kb on - strand, within BT1543at 1903.703 kb on + strand, within BT1543at 1903.704 kb on - strand, within BT1543at 1903.809 kb on + strand, within BT1543at 1903.893 kb on + strand, within BT1543at 1904.267 kb on + strand, within BT1543at 1904.272 kb on - strand, within BT1543at 1904.330 kb on + strand, within BT1543at 1904.367 kb on + strand, within BT1543at 1904.391 kb on + strand, within BT1543at 1904.482 kb on + strand, within BT1543at 1904.483 kb on - strand, within BT1543at 1904.483 kb on - strand, within BT1543at 1904.501 kb on - strandat 1904.532 kb on + strandat 1904.533 kb on - strandat 1904.551 kb on + strandat 1904.579 kb on - strandat 1904.607 kb on - strandat 1904.641 kb on - strandat 1904.652 kb on - strandat 1904.686 kb on - strandat 1904.686 kb on - strandat 1904.688 kb on - strandat 1904.868 kb on + strand, within BT1544at 1904.869 kb on - strand, within BT1544at 1904.869 kb on - strand, within BT1544at 1904.873 kb on + strand, within BT1544at 1904.932 kb on - strand, within BT1544at 1904.950 kb on + strand, within BT1544at 1905.002 kb on - strand, within BT1544at 1905.097 kb on - strand, within BT1544at 1905.185 kb on + strand, within BT1544at 1905.385 kb on + strand, within BT1544at 1905.523 kb on - strandat 1905.647 kb on + strand, within BT1545at 1905.647 kb on + strand, within BT1545at 1905.647 kb on + strand, within BT1545

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.01 mM
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1,902,070 + BT1542 0.69 -1.2
1,902,164 + BT1542 0.76 -6.0
1,902,330 + BT1542 0.88 +0.8
1,902,330 + BT1542 0.88 -0.2
1,902,345 + BT1542 0.89 +0.2
1,902,345 + BT1542 0.89 -0.7
1,902,345 + BT1542 0.89 -0.4
1,902,346 - BT1542 0.90 -0.6
1,902,381 + -0.7
1,902,382 - +0.0
1,902,418 - -0.8
1,902,418 - -1.0
1,902,486 - +0.6
1,902,611 + +0.0
1,902,612 - +0.2
1,902,617 + +0.2
1,902,667 - -2.7
1,902,688 - -0.1
1,902,694 - -1.0
1,902,694 - -1.6
1,902,698 - -0.4
1,902,698 - +0.0
1,902,831 - +0.2
1,902,845 + -1.4
1,902,867 + -0.5
1,902,867 + +0.1
1,902,867 + +0.6
1,902,868 - -1.0
1,902,871 + +0.4
1,902,872 - +1.6
1,902,898 - -0.7
1,902,912 - +0.6
1,902,915 - -1.3
1,902,984 - +0.6
1,903,064 - -1.0
1,903,064 - -1.6
1,903,083 - +2.0
1,903,104 + -1.0
1,903,183 + -0.3
1,903,256 + BT1543 0.13 -0.4
1,903,280 + BT1543 0.14 -1.8
1,903,314 + BT1543 0.16 +0.0
1,903,321 + BT1543 0.17 -1.2
1,903,343 - BT1543 0.18 -1.1
1,903,343 - BT1543 0.18 +0.0
1,903,460 + BT1543 0.25 -1.4
1,903,513 + BT1543 0.29 +0.6
1,903,513 + BT1543 0.29 -0.7
1,903,539 + BT1543 0.30 -1.0
1,903,592 + BT1543 0.34 -0.2
1,903,671 + BT1543 0.39 -1.2
1,903,701 - BT1543 0.40 -0.4
1,903,703 + BT1543 0.41 -0.7
1,903,704 - BT1543 0.41 -2.0
1,903,809 + BT1543 0.47 +0.0
1,903,893 + BT1543 0.52 -1.2
1,904,267 + BT1543 0.76 -0.8
1,904,272 - BT1543 0.76 -0.9
1,904,330 + BT1543 0.80 -1.4
1,904,367 + BT1543 0.82 +1.6
1,904,391 + BT1543 0.84 +0.6
1,904,482 + BT1543 0.89 -1.2
1,904,483 - BT1543 0.89 -2.3
1,904,483 - BT1543 0.89 -0.9
1,904,501 - -0.4
1,904,532 + -1.7
1,904,533 - -0.9
1,904,551 + -2.1
1,904,579 - -0.5
1,904,607 - -0.7
1,904,641 - -0.2
1,904,652 - -0.6
1,904,686 - -0.6
1,904,686 - -1.6
1,904,688 - -0.7
1,904,868 + BT1544 0.16 -0.4
1,904,869 - BT1544 0.16 -0.9
1,904,869 - BT1544 0.16 +0.3
1,904,873 + BT1544 0.17 +1.3
1,904,932 - BT1544 0.24 -0.7
1,904,950 + BT1544 0.27 -1.4
1,905,002 - BT1544 0.33 +2.6
1,905,097 - BT1544 0.45 -1.3
1,905,185 + BT1544 0.56 -0.4
1,905,385 + BT1544 0.82 -0.9
1,905,523 - -1.7
1,905,647 + BT1545 0.20 -0.5
1,905,647 + BT1545 0.20 +0.6
1,905,647 + BT1545 0.20 -0.4

Or see this region's nucleotide sequence