Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_6090

Experiment: LB no stress 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_6088 and Pf6N2E2_6089 are separated by 72 nucleotidesPf6N2E2_6089 and Pf6N2E2_6090 are separated by 99 nucleotidesPf6N2E2_6090 and Pf6N2E2_6091 overlap by 32 nucleotidesPf6N2E2_6091 and Pf6N2E2_6092 are separated by 28 nucleotides Pf6N2E2_6088 - hypothetical protein, at 6,912,888 to 6,913,145 _6088 Pf6N2E2_6089 - ThiJ/PfpI family protein, at 6,913,218 to 6,913,904 _6089 Pf6N2E2_6090 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 6,914,004 to 6,914,996 _6090 Pf6N2E2_6091 - Epoxide hydrolase (EC 3.3.2.9), at 6,914,965 to 6,915,867 _6091 Pf6N2E2_6092 - hypothetical protein, at 6,915,896 to 6,916,273 _6092 Position (kb) 6914 6915Strain fitness (log2 ratio) -2 -1 0 1at 6913.014 kb on + strand, within Pf6N2E2_6088at 6913.015 kb on - strand, within Pf6N2E2_6088at 6913.015 kb on - strand, within Pf6N2E2_6088at 6913.027 kb on - strand, within Pf6N2E2_6088at 6913.054 kb on - strand, within Pf6N2E2_6088at 6913.364 kb on - strand, within Pf6N2E2_6089at 6913.534 kb on + strand, within Pf6N2E2_6089at 6913.535 kb on - strand, within Pf6N2E2_6089at 6913.618 kb on + strand, within Pf6N2E2_6089at 6913.618 kb on + strand, within Pf6N2E2_6089at 6913.619 kb on - strand, within Pf6N2E2_6089at 6913.619 kb on - strand, within Pf6N2E2_6089at 6913.619 kb on - strand, within Pf6N2E2_6089at 6913.833 kb on + strand, within Pf6N2E2_6089at 6913.834 kb on - strand, within Pf6N2E2_6089at 6913.840 kb on + strandat 6913.841 kb on - strandat 6913.953 kb on - strandat 6914.003 kb on + strandat 6914.134 kb on - strand, within Pf6N2E2_6090at 6914.259 kb on + strand, within Pf6N2E2_6090at 6914.735 kb on + strand, within Pf6N2E2_6090at 6914.946 kb on + strandat 6915.059 kb on + strand, within Pf6N2E2_6091at 6915.059 kb on + strand, within Pf6N2E2_6091at 6915.212 kb on + strand, within Pf6N2E2_6091at 6915.212 kb on + strand, within Pf6N2E2_6091at 6915.212 kb on + strand, within Pf6N2E2_6091at 6915.212 kb on + strand, within Pf6N2E2_6091at 6915.213 kb on - strand, within Pf6N2E2_6091at 6915.213 kb on - strand, within Pf6N2E2_6091at 6915.230 kb on + strand, within Pf6N2E2_6091at 6915.231 kb on - strand, within Pf6N2E2_6091at 6915.398 kb on + strand, within Pf6N2E2_6091at 6915.398 kb on + strand, within Pf6N2E2_6091at 6915.398 kb on + strand, within Pf6N2E2_6091at 6915.398 kb on + strand, within Pf6N2E2_6091at 6915.399 kb on - strand, within Pf6N2E2_6091at 6915.399 kb on - strand, within Pf6N2E2_6091at 6915.401 kb on + strand, within Pf6N2E2_6091at 6915.402 kb on - strand, within Pf6N2E2_6091at 6915.473 kb on + strand, within Pf6N2E2_6091at 6915.918 kb on + strandat 6915.918 kb on + strandat 6915.919 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 24 hours
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6,913,014 + Pf6N2E2_6088 0.49 +0.8
6,913,015 - Pf6N2E2_6088 0.49 +0.2
6,913,015 - Pf6N2E2_6088 0.49 -0.5
6,913,027 - Pf6N2E2_6088 0.54 -0.6
6,913,054 - Pf6N2E2_6088 0.64 +1.0
6,913,364 - Pf6N2E2_6089 0.21 +0.3
6,913,534 + Pf6N2E2_6089 0.46 -1.9
6,913,535 - Pf6N2E2_6089 0.46 +0.4
6,913,618 + Pf6N2E2_6089 0.58 +1.0
6,913,618 + Pf6N2E2_6089 0.58 -0.1
6,913,619 - Pf6N2E2_6089 0.58 +0.4
6,913,619 - Pf6N2E2_6089 0.58 +1.0
6,913,619 - Pf6N2E2_6089 0.58 +1.3
6,913,833 + Pf6N2E2_6089 0.90 -0.1
6,913,834 - Pf6N2E2_6089 0.90 +0.4
6,913,840 + +0.3
6,913,841 - +0.7
6,913,953 - +0.2
6,914,003 + +0.8
6,914,134 - Pf6N2E2_6090 0.13 +0.1
6,914,259 + Pf6N2E2_6090 0.26 -0.4
6,914,735 + Pf6N2E2_6090 0.74 -1.8
6,914,946 + +0.2
6,915,059 + Pf6N2E2_6091 0.10 -0.7
6,915,059 + Pf6N2E2_6091 0.10 -0.7
6,915,212 + Pf6N2E2_6091 0.27 -0.4
6,915,212 + Pf6N2E2_6091 0.27 -0.1
6,915,212 + Pf6N2E2_6091 0.27 -0.4
6,915,212 + Pf6N2E2_6091 0.27 -0.8
6,915,213 - Pf6N2E2_6091 0.27 -0.3
6,915,213 - Pf6N2E2_6091 0.27 +1.4
6,915,230 + Pf6N2E2_6091 0.29 +0.9
6,915,231 - Pf6N2E2_6091 0.29 +0.5
6,915,398 + Pf6N2E2_6091 0.48 +0.5
6,915,398 + Pf6N2E2_6091 0.48 +0.7
6,915,398 + Pf6N2E2_6091 0.48 +0.7
6,915,398 + Pf6N2E2_6091 0.48 -0.1
6,915,399 - Pf6N2E2_6091 0.48 +0.8
6,915,399 - Pf6N2E2_6091 0.48 +1.3
6,915,401 + Pf6N2E2_6091 0.48 +0.7
6,915,402 - Pf6N2E2_6091 0.48 +0.9
6,915,473 + Pf6N2E2_6091 0.56 +0.6
6,915,918 + +0.2
6,915,918 + +0.7
6,915,919 - -0.9

Or see this region's nucleotide sequence