Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6060 and Pf6N2E2_6061 overlap by 4 nucleotides Pf6N2E2_6061 and Pf6N2E2_6062 are separated by 41 nucleotides Pf6N2E2_6062 and Pf6N2E2_6063 are separated by 177 nucleotides
Pf6N2E2_6060 - Propionate catabolism operon transcriptional regulator of GntR family [predicted], at 6,880,154 to 6,880,864
_6060
Pf6N2E2_6061 - Methylisocitrate lyase (EC 4.1.3.30) (from data) , at 6,880,861 to 6,881,745
_6061
Pf6N2E2_6062 - 2-methylcitrate synthase (EC 2.3.3.5) (from data) , at 6,881,787 to 6,882,914
_6062
Pf6N2E2_6063 - 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79), at 6,883,092 to 6,885,683
_6063
Position (kb)
6881
6882
6883 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6880.987 kb on + strand, within Pf6N2E2_6061 at 6880.987 kb on + strand, within Pf6N2E2_6061 at 6880.988 kb on - strand, within Pf6N2E2_6061 at 6880.988 kb on - strand, within Pf6N2E2_6061 at 6881.036 kb on + strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.200 kb on - strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.519 kb on - strand, within Pf6N2E2_6061 at 6881.611 kb on + strand, within Pf6N2E2_6061 at 6881.612 kb on - strand, within Pf6N2E2_6061 at 6881.612 kb on - strand, within Pf6N2E2_6061 at 6881.695 kb on + strand at 6881.695 kb on + strand at 6881.743 kb on + strand at 6881.743 kb on + strand at 6881.744 kb on - strand at 6881.744 kb on - strand at 6881.744 kb on - strand at 6881.803 kb on + strand at 6881.803 kb on + strand at 6881.886 kb on + strand at 6881.991 kb on + strand, within Pf6N2E2_6062 at 6881.992 kb on - strand, within Pf6N2E2_6062 at 6882.193 kb on - strand, within Pf6N2E2_6062 at 6882.252 kb on - strand, within Pf6N2E2_6062 at 6882.389 kb on + strand, within Pf6N2E2_6062 at 6882.389 kb on + strand, within Pf6N2E2_6062 at 6882.390 kb on - strand, within Pf6N2E2_6062 at 6882.390 kb on - strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.577 kb on - strand, within Pf6N2E2_6062 at 6882.578 kb on + strand, within Pf6N2E2_6062 at 6882.726 kb on + strand, within Pf6N2E2_6062 at 6882.727 kb on - strand, within Pf6N2E2_6062 at 6882.867 kb on + strand at 6882.868 kb on - strand at 6882.935 kb on - strand at 6882.935 kb on - strand at 6882.935 kb on - strand at 6883.137 kb on + strand at 6883.182 kb on + strand at 6883.183 kb on - strand at 6883.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 6,880,987 + Pf6N2E2_6061 0.14 -0.1 6,880,987 + Pf6N2E2_6061 0.14 -1.6 6,880,988 - Pf6N2E2_6061 0.14 -1.1 6,880,988 - Pf6N2E2_6061 0.14 -0.2 6,881,036 + Pf6N2E2_6061 0.20 -0.8 6,881,037 - Pf6N2E2_6061 0.20 +0.6 6,881,037 - Pf6N2E2_6061 0.20 +0.5 6,881,037 - Pf6N2E2_6061 0.20 -0.9 6,881,200 - Pf6N2E2_6061 0.38 +1.0 6,881,446 + Pf6N2E2_6061 0.66 +0.3 6,881,446 + Pf6N2E2_6061 0.66 +0.3 6,881,446 + Pf6N2E2_6061 0.66 -0.2 6,881,446 + Pf6N2E2_6061 0.66 -3.0 6,881,446 + Pf6N2E2_6061 0.66 +0.5 6,881,446 + Pf6N2E2_6061 0.66 +0.6 6,881,447 - Pf6N2E2_6061 0.66 -0.1 6,881,447 - Pf6N2E2_6061 0.66 +0.8 6,881,447 - Pf6N2E2_6061 0.66 -0.8 6,881,447 - Pf6N2E2_6061 0.66 -0.7 6,881,447 - Pf6N2E2_6061 0.66 -0.6 6,881,447 - Pf6N2E2_6061 0.66 +1.1 6,881,447 - Pf6N2E2_6061 0.66 -0.2 6,881,519 - Pf6N2E2_6061 0.74 -0.1 6,881,611 + Pf6N2E2_6061 0.85 -1.0 6,881,612 - Pf6N2E2_6061 0.85 -1.3 6,881,612 - Pf6N2E2_6061 0.85 +0.5 6,881,695 + +0.3 6,881,695 + -0.2 6,881,743 + +0.4 6,881,743 + -0.7 6,881,744 - +0.5 6,881,744 - -0.6 6,881,744 - -0.1 6,881,803 + -0.1 6,881,803 + -0.2 6,881,886 + +0.1 6,881,991 + Pf6N2E2_6062 0.18 -0.8 6,881,992 - Pf6N2E2_6062 0.18 -0.2 6,882,193 - Pf6N2E2_6062 0.36 -1.1 6,882,252 - Pf6N2E2_6062 0.41 -1.1 6,882,389 + Pf6N2E2_6062 0.53 -0.4 6,882,389 + Pf6N2E2_6062 0.53 -1.5 6,882,390 - Pf6N2E2_6062 0.53 -0.5 6,882,390 - Pf6N2E2_6062 0.53 -0.4 6,882,576 + Pf6N2E2_6062 0.70 -1.3 6,882,576 + Pf6N2E2_6062 0.70 -1.9 6,882,576 + Pf6N2E2_6062 0.70 -0.2 6,882,576 + Pf6N2E2_6062 0.70 -1.0 6,882,576 + Pf6N2E2_6062 0.70 +0.5 6,882,577 - Pf6N2E2_6062 0.70 -0.9 6,882,578 + Pf6N2E2_6062 0.70 -1.8 6,882,726 + Pf6N2E2_6062 0.83 +0.2 6,882,727 - Pf6N2E2_6062 0.83 -1.2 6,882,867 + -1.8 6,882,868 - -0.7 6,882,935 - +0.4 6,882,935 - -0.4 6,882,935 - -0.1 6,883,137 + -2.2 6,883,182 + -0.5 6,883,183 - +0.6 6,883,197 + +0.1
Or see this region's nucleotide sequence