Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4924

Experiment: LB no stress 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4922 and Pf6N2E2_4923 overlap by 4 nucleotidesPf6N2E2_4923 and Pf6N2E2_4924 are separated by 18 nucleotidesPf6N2E2_4924 and Pf6N2E2_4925 overlap by 4 nucleotidesPf6N2E2_4925 and Pf6N2E2_4926 are separated by 59 nucleotides Pf6N2E2_4922 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 5,676,579 to 5,677,055 _4922 Pf6N2E2_4923 - Transcription termination protein NusB, at 5,677,052 to 5,677,552 _4923 Pf6N2E2_4924 - Thiamine-monophosphate kinase (EC 2.7.4.16), at 5,677,571 to 5,678,536 _4924 Pf6N2E2_4925 - Phosphatidylglycerophosphatase A (EC 3.1.3.27), at 5,678,533 to 5,679,036 _4925 Pf6N2E2_4926 - hypothetical protein, at 5,679,096 to 5,679,806 _4926 Position (kb) 5677 5678 5679Strain fitness (log2 ratio) -2 -1 0 1 2at 5679.067 kb on + strandat 5679.068 kb on - strandat 5679.068 kb on - strandat 5679.068 kb on - strandat 5679.086 kb on - strandat 5679.203 kb on + strand, within Pf6N2E2_4926at 5679.361 kb on - strand, within Pf6N2E2_4926at 5679.361 kb on - strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.374 kb on + strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.375 kb on - strand, within Pf6N2E2_4926at 5679.378 kb on + strand, within Pf6N2E2_4926at 5679.378 kb on + strand, within Pf6N2E2_4926at 5679.378 kb on + strand, within Pf6N2E2_4926at 5679.378 kb on + strand, within Pf6N2E2_4926at 5679.378 kb on + strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.379 kb on - strand, within Pf6N2E2_4926at 5679.432 kb on + strand, within Pf6N2E2_4926at 5679.433 kb on - strand, within Pf6N2E2_4926at 5679.433 kb on - strand, within Pf6N2E2_4926at 5679.462 kb on + strand, within Pf6N2E2_4926at 5679.462 kb on + strand, within Pf6N2E2_4926at 5679.471 kb on + strand, within Pf6N2E2_4926at 5679.471 kb on + strand, within Pf6N2E2_4926at 5679.471 kb on + strand, within Pf6N2E2_4926at 5679.472 kb on - strand, within Pf6N2E2_4926at 5679.472 kb on - strand, within Pf6N2E2_4926at 5679.472 kb on - strand, within Pf6N2E2_4926at 5679.472 kb on - strand, within Pf6N2E2_4926at 5679.515 kb on - strand, within Pf6N2E2_4926at 5679.518 kb on + strand, within Pf6N2E2_4926at 5679.518 kb on + strand, within Pf6N2E2_4926at 5679.518 kb on + strand, within Pf6N2E2_4926at 5679.519 kb on - strand, within Pf6N2E2_4926at 5679.519 kb on - strand, within Pf6N2E2_4926

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 24 hours
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5,679,067 + -0.1
5,679,068 - -1.3
5,679,068 - -0.8
5,679,068 - +0.4
5,679,086 - +0.8
5,679,203 + Pf6N2E2_4926 0.15 -0.0
5,679,361 - Pf6N2E2_4926 0.37 -0.5
5,679,361 - Pf6N2E2_4926 0.37 +0.7
5,679,374 + Pf6N2E2_4926 0.39 +0.5
5,679,374 + Pf6N2E2_4926 0.39 +0.3
5,679,374 + Pf6N2E2_4926 0.39 -2.4
5,679,374 + Pf6N2E2_4926 0.39 -0.4
5,679,374 + Pf6N2E2_4926 0.39 -0.1
5,679,374 + Pf6N2E2_4926 0.39 -0.9
5,679,374 + Pf6N2E2_4926 0.39 +0.7
5,679,374 + Pf6N2E2_4926 0.39 +2.2
5,679,374 + Pf6N2E2_4926 0.39 +0.1
5,679,374 + Pf6N2E2_4926 0.39 -0.5
5,679,375 - Pf6N2E2_4926 0.39 -0.2
5,679,375 - Pf6N2E2_4926 0.39 +0.6
5,679,375 - Pf6N2E2_4926 0.39 +0.1
5,679,375 - Pf6N2E2_4926 0.39 -1.2
5,679,375 - Pf6N2E2_4926 0.39 +0.1
5,679,375 - Pf6N2E2_4926 0.39 +0.5
5,679,375 - Pf6N2E2_4926 0.39 -1.0
5,679,375 - Pf6N2E2_4926 0.39 +1.4
5,679,375 - Pf6N2E2_4926 0.39 -0.1
5,679,375 - Pf6N2E2_4926 0.39 -0.5
5,679,375 - Pf6N2E2_4926 0.39 -0.7
5,679,375 - Pf6N2E2_4926 0.39 -0.1
5,679,378 + Pf6N2E2_4926 0.40 -0.1
5,679,378 + Pf6N2E2_4926 0.40 -0.2
5,679,378 + Pf6N2E2_4926 0.40 -0.6
5,679,378 + Pf6N2E2_4926 0.40 -0.0
5,679,378 + Pf6N2E2_4926 0.40 -0.0
5,679,379 - Pf6N2E2_4926 0.40 +0.1
5,679,379 - Pf6N2E2_4926 0.40 -0.3
5,679,379 - Pf6N2E2_4926 0.40 -0.2
5,679,379 - Pf6N2E2_4926 0.40 -0.5
5,679,379 - Pf6N2E2_4926 0.40 +0.1
5,679,379 - Pf6N2E2_4926 0.40 +0.3
5,679,379 - Pf6N2E2_4926 0.40 +0.7
5,679,379 - Pf6N2E2_4926 0.40 +0.1
5,679,379 - Pf6N2E2_4926 0.40 +0.5
5,679,379 - Pf6N2E2_4926 0.40 -0.6
5,679,432 + Pf6N2E2_4926 0.47 +0.7
5,679,433 - Pf6N2E2_4926 0.47 -0.1
5,679,433 - Pf6N2E2_4926 0.47 -0.5
5,679,462 + Pf6N2E2_4926 0.51 +0.7
5,679,462 + Pf6N2E2_4926 0.51 -0.4
5,679,471 + Pf6N2E2_4926 0.53 +0.2
5,679,471 + Pf6N2E2_4926 0.53 +0.5
5,679,471 + Pf6N2E2_4926 0.53 +0.1
5,679,472 - Pf6N2E2_4926 0.53 -0.7
5,679,472 - Pf6N2E2_4926 0.53 +0.9
5,679,472 - Pf6N2E2_4926 0.53 +0.3
5,679,472 - Pf6N2E2_4926 0.53 -1.0
5,679,515 - Pf6N2E2_4926 0.59 -0.5
5,679,518 + Pf6N2E2_4926 0.59 -1.2
5,679,518 + Pf6N2E2_4926 0.59 +0.1
5,679,518 + Pf6N2E2_4926 0.59 +0.4
5,679,519 - Pf6N2E2_4926 0.59 +0.7
5,679,519 - Pf6N2E2_4926 0.59 -1.3

Or see this region's nucleotide sequence