Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_46

Experiment: LB no stress 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_45 and Pf6N2E2_46 are separated by 54 nucleotidesPf6N2E2_46 and Pf6N2E2_47 overlap by 4 nucleotidesPf6N2E2_47 and Pf6N2E2_48 are separated by 89 nucleotidesPf6N2E2_48 and Pf6N2E2_6095 are separated by 251 nucleotides Pf6N2E2_45 - Putative protease, at 84,785 to 86,209 _45 Pf6N2E2_46 - membrane protein, putative, at 86,264 to 87,325 _46 Pf6N2E2_47 - FIG00961567: hypothetical protein, at 87,322 to 87,504 _47 Pf6N2E2_48 - FIG00955661: hypothetical protein, at 87,594 to 87,788 _48 Pf6N2E2_6095 - tRNA-Asn-GTT, at 88,040 to 88,112 _6095 Position (kb) 86 87 88Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.483 kb on + strand, within Pf6N2E2_45at 85.510 kb on - strand, within Pf6N2E2_45at 85.510 kb on - strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.593 kb on + strand, within Pf6N2E2_45at 85.594 kb on - strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.924 kb on - strand, within Pf6N2E2_45at 85.924 kb on - strand, within Pf6N2E2_45at 85.924 kb on - strand, within Pf6N2E2_45at 86.010 kb on + strand, within Pf6N2E2_45at 86.028 kb on + strand, within Pf6N2E2_45at 86.028 kb on + strand, within Pf6N2E2_45at 86.230 kb on + strandat 86.230 kb on + strandat 86.230 kb on + strandat 86.230 kb on + strandat 86.231 kb on - strandat 86.231 kb on - strandat 86.247 kb on + strandat 86.247 kb on + strandat 86.506 kb on + strand, within Pf6N2E2_46at 86.506 kb on + strand, within Pf6N2E2_46at 86.537 kb on + strand, within Pf6N2E2_46at 86.537 kb on + strand, within Pf6N2E2_46at 86.538 kb on - strand, within Pf6N2E2_46at 86.538 kb on - strand, within Pf6N2E2_46at 86.771 kb on + strand, within Pf6N2E2_46at 86.771 kb on + strand, within Pf6N2E2_46at 86.772 kb on - strand, within Pf6N2E2_46at 86.772 kb on - strand, within Pf6N2E2_46at 86.816 kb on - strand, within Pf6N2E2_46at 87.059 kb on + strand, within Pf6N2E2_46at 87.060 kb on - strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46at 87.323 kb on + strandat 87.338 kb on + strandat 87.339 kb on - strandat 87.531 kb on + strandat 87.531 kb on + strandat 87.536 kb on + strandat 87.536 kb on + strandat 87.536 kb on + strandat 87.575 kb on + strandat 87.575 kb on + strandat 87.575 kb on + strandat 87.576 kb on - strandat 87.578 kb on + strandat 87.579 kb on - strandat 87.733 kb on + strand, within Pf6N2E2_48at 87.734 kb on - strand, within Pf6N2E2_48at 87.734 kb on - strand, within Pf6N2E2_48at 87.788 kb on + strandat 87.788 kb on + strandat 87.806 kb on + strandat 87.806 kb on + strandat 87.807 kb on - strandat 87.906 kb on + strandat 87.928 kb on + strandat 87.929 kb on - strandat 87.975 kb on + strandat 88.034 kb on - strandat 88.077 kb on - strand, within Pf6N2E2_6095at 88.077 kb on - strand, within Pf6N2E2_6095at 88.093 kb on - strand, within Pf6N2E2_6095at 88.093 kb on - strand, within Pf6N2E2_6095at 88.126 kb on + strandat 88.233 kb on + strandat 88.234 kb on - strandat 88.285 kb on + strandat 88.286 kb on - strandat 88.286 kb on - strandat 88.286 kb on - strandat 88.286 kb on - strandat 88.291 kb on - strandat 88.291 kb on - strandat 88.311 kb on + strandat 88.312 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 24 hours
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85,344 + Pf6N2E2_45 0.39 -0.2
85,344 + Pf6N2E2_45 0.39 +0.3
85,344 + Pf6N2E2_45 0.39 +2.7
85,344 + Pf6N2E2_45 0.39 +1.3
85,344 + Pf6N2E2_45 0.39 +1.1
85,344 + Pf6N2E2_45 0.39 +0.3
85,344 + Pf6N2E2_45 0.39 -0.5
85,345 - Pf6N2E2_45 0.39 +1.2
85,345 - Pf6N2E2_45 0.39 -1.6
85,345 - Pf6N2E2_45 0.39 +0.5
85,345 - Pf6N2E2_45 0.39 +0.9
85,345 - Pf6N2E2_45 0.39 -0.4
85,345 - Pf6N2E2_45 0.39 +0.1
85,458 + Pf6N2E2_45 0.47 +1.1
85,458 + Pf6N2E2_45 0.47 +0.1
85,458 + Pf6N2E2_45 0.47 +1.4
85,459 - Pf6N2E2_45 0.47 -0.7
85,459 - Pf6N2E2_45 0.47 -0.1
85,459 - Pf6N2E2_45 0.47 +0.8
85,459 - Pf6N2E2_45 0.47 +1.1
85,459 - Pf6N2E2_45 0.47 -2.0
85,483 + Pf6N2E2_45 0.49 +0.8
85,510 - Pf6N2E2_45 0.51 +0.8
85,510 - Pf6N2E2_45 0.51 +0.0
85,562 + Pf6N2E2_45 0.55 +0.6
85,562 + Pf6N2E2_45 0.55 +0.2
85,562 + Pf6N2E2_45 0.55 +0.4
85,593 + Pf6N2E2_45 0.57 +0.0
85,594 - Pf6N2E2_45 0.57 +0.8
85,923 + Pf6N2E2_45 0.80 -1.0
85,923 + Pf6N2E2_45 0.80 +0.1
85,923 + Pf6N2E2_45 0.80 +0.3
85,923 + Pf6N2E2_45 0.80 +0.9
85,923 + Pf6N2E2_45 0.80 +0.1
85,924 - Pf6N2E2_45 0.80 -0.4
85,924 - Pf6N2E2_45 0.80 +0.6
85,924 - Pf6N2E2_45 0.80 -0.6
86,010 + Pf6N2E2_45 0.86 +0.5
86,028 + Pf6N2E2_45 0.87 -0.7
86,028 + Pf6N2E2_45 0.87 +0.4
86,230 + -0.3
86,230 + +0.5
86,230 + -0.6
86,230 + +0.5
86,231 - +0.1
86,231 - +0.1
86,247 + -1.1
86,247 + +0.6
86,506 + Pf6N2E2_46 0.23 -0.3
86,506 + Pf6N2E2_46 0.23 -0.1
86,537 + Pf6N2E2_46 0.26 -0.7
86,537 + Pf6N2E2_46 0.26 +0.3
86,538 - Pf6N2E2_46 0.26 -0.7
86,538 - Pf6N2E2_46 0.26 -1.0
86,771 + Pf6N2E2_46 0.48 -0.2
86,771 + Pf6N2E2_46 0.48 +0.1
86,772 - Pf6N2E2_46 0.48 +0.9
86,772 - Pf6N2E2_46 0.48 +0.2
86,816 - Pf6N2E2_46 0.52 +0.0
87,059 + Pf6N2E2_46 0.75 -0.0
87,060 - Pf6N2E2_46 0.75 +0.5
87,185 + Pf6N2E2_46 0.87 +1.0
87,185 + Pf6N2E2_46 0.87 +0.8
87,185 + Pf6N2E2_46 0.87 +0.4
87,185 + Pf6N2E2_46 0.87 +1.0
87,185 + Pf6N2E2_46 0.87 +1.3
87,186 - Pf6N2E2_46 0.87 -0.2
87,186 - Pf6N2E2_46 0.87 +1.4
87,186 - Pf6N2E2_46 0.87 -0.6
87,323 + -1.3
87,338 + +1.7
87,339 - -0.3
87,531 + -0.2
87,531 + -0.8
87,536 + +1.3
87,536 + -1.6
87,536 + -0.3
87,575 + -0.5
87,575 + -0.8
87,575 + -0.3
87,576 - -0.5
87,578 + -0.5
87,579 - -1.0
87,733 + Pf6N2E2_48 0.71 -0.7
87,734 - Pf6N2E2_48 0.72 -1.3
87,734 - Pf6N2E2_48 0.72 -0.8
87,788 + -0.2
87,788 + -0.6
87,806 + +1.7
87,806 + +0.1
87,807 - -1.8
87,906 + +0.4
87,928 + +0.8
87,929 - -0.9
87,975 + +0.3
88,034 - -0.4
88,077 - Pf6N2E2_6095 0.51 -3.1
88,077 - Pf6N2E2_6095 0.51 +0.3
88,093 - Pf6N2E2_6095 0.73 +0.1
88,093 - Pf6N2E2_6095 0.73 +0.7
88,126 + +0.2
88,233 + +0.4
88,234 - -0.8
88,285 + +2.2
88,286 - +0.4
88,286 - +0.1
88,286 - +0.9
88,286 - +0.4
88,291 - -1.3
88,291 - +0.8
88,311 + -1.2
88,312 - +0.8

Or see this region's nucleotide sequence