Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2370 and Pf6N2E2_2371 overlap by 4 nucleotides Pf6N2E2_2371 and Pf6N2E2_2372 overlap by 4 nucleotides
Pf6N2E2_2370 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 2,803,616 to 2,805,673
_2370
Pf6N2E2_2371 - Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE), at 2,805,670 to 2,806,773
_2371
Pf6N2E2_2372 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 2,806,770 to 2,808,425
_2372
Position (kb)
2805
2806
2807 Strain fitness (log2 ratio)
-1
0
1 at 2804.729 kb on + strand, within Pf6N2E2_2370 at 2804.730 kb on - strand, within Pf6N2E2_2370 at 2804.980 kb on - strand, within Pf6N2E2_2370 at 2805.059 kb on - strand, within Pf6N2E2_2370 at 2805.059 kb on - strand, within Pf6N2E2_2370 at 2805.120 kb on + strand, within Pf6N2E2_2370 at 2805.121 kb on - strand, within Pf6N2E2_2370 at 2805.499 kb on - strand at 2805.499 kb on - strand at 2805.717 kb on - strand at 2805.908 kb on + strand, within Pf6N2E2_2371 at 2806.119 kb on + strand, within Pf6N2E2_2371 at 2806.120 kb on - strand, within Pf6N2E2_2371 at 2806.792 kb on + strand at 2806.792 kb on + strand at 2806.830 kb on + strand at 2806.830 kb on + strand at 2806.830 kb on + strand at 2806.831 kb on - strand at 2806.831 kb on - strand at 2807.061 kb on - strand, within Pf6N2E2_2372 at 2807.190 kb on + strand, within Pf6N2E2_2372 at 2807.190 kb on + strand, within Pf6N2E2_2372 at 2807.191 kb on - strand, within Pf6N2E2_2372 at 2807.191 kb on - strand, within Pf6N2E2_2372 at 2807.209 kb on + strand, within Pf6N2E2_2372 at 2807.209 kb on + strand, within Pf6N2E2_2372 at 2807.210 kb on - strand, within Pf6N2E2_2372 at 2807.257 kb on + strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,804,729 + Pf6N2E2_2370 0.54 +0.6 2,804,730 - Pf6N2E2_2370 0.54 -0.6 2,804,980 - Pf6N2E2_2370 0.66 +0.5 2,805,059 - Pf6N2E2_2370 0.70 +1.2 2,805,059 - Pf6N2E2_2370 0.70 +0.5 2,805,120 + Pf6N2E2_2370 0.73 +0.1 2,805,121 - Pf6N2E2_2370 0.73 -0.1 2,805,499 - +0.2 2,805,499 - +0.2 2,805,717 - -0.5 2,805,908 + Pf6N2E2_2371 0.22 -0.5 2,806,119 + Pf6N2E2_2371 0.41 -0.4 2,806,120 - Pf6N2E2_2371 0.41 -0.9 2,806,792 + -0.9 2,806,792 + +0.3 2,806,830 + +0.6 2,806,830 + +1.0 2,806,830 + +0.1 2,806,831 - +0.1 2,806,831 - +0.3 2,807,061 - Pf6N2E2_2372 0.18 +1.5 2,807,190 + Pf6N2E2_2372 0.25 +0.8 2,807,190 + Pf6N2E2_2372 0.25 -0.6 2,807,191 - Pf6N2E2_2372 0.25 +0.4 2,807,191 - Pf6N2E2_2372 0.25 -0.5 2,807,209 + Pf6N2E2_2372 0.27 +0.1 2,807,209 + Pf6N2E2_2372 0.27 -1.5 2,807,210 - Pf6N2E2_2372 0.27 +0.4 2,807,257 + Pf6N2E2_2372 0.29 +0.7 2,807,258 - Pf6N2E2_2372 0.29 +0.6 2,807,258 - Pf6N2E2_2372 0.29 +0.3 2,807,258 - Pf6N2E2_2372 0.29 -0.2 2,807,258 - Pf6N2E2_2372 0.29 +0.8 2,807,258 - Pf6N2E2_2372 0.29 +0.3 2,807,258 - Pf6N2E2_2372 0.29 -0.4 2,807,258 - Pf6N2E2_2372 0.29 -0.9 2,807,258 - Pf6N2E2_2372 0.29 +0.1
Or see this region's nucleotide sequence