Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1833 and Pf6N2E2_1834 are separated by 2 nucleotides Pf6N2E2_1834 and Pf6N2E2_1835 overlap by 4 nucleotides Pf6N2E2_1835 and Pf6N2E2_1836 are separated by 170 nucleotides
Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668
_1833
Pf6N2E2_1834 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,172,671 to 2,173,441
_1834
Pf6N2E2_1835 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,173,438 to 2,174,049
_1835
Pf6N2E2_1836 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 2,174,220 to 2,175,422
_1836
Position (kb)
2172
2173
2174 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2172.079 kb on + strand, within Pf6N2E2_1833 at 2172.081 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.317 kb on + strand, within Pf6N2E2_1833 at 2172.317 kb on + strand, within Pf6N2E2_1833 at 2172.318 kb on - strand, within Pf6N2E2_1833 at 2172.318 kb on - strand, within Pf6N2E2_1833 at 2172.506 kb on + strand, within Pf6N2E2_1833 at 2172.574 kb on + strand at 2172.575 kb on - strand at 2172.575 kb on - strand at 2172.663 kb on - strand at 2172.668 kb on + strand at 2172.668 kb on + strand at 2172.669 kb on - strand at 2172.669 kb on - strand at 2172.672 kb on + strand at 2172.673 kb on - strand at 2173.007 kb on + strand, within Pf6N2E2_1834 at 2173.239 kb on - strand, within Pf6N2E2_1834 at 2173.399 kb on + strand at 2173.442 kb on - strand at 2173.442 kb on - strand at 2173.448 kb on + strand at 2173.675 kb on + strand, within Pf6N2E2_1835 at 2173.676 kb on - strand, within Pf6N2E2_1835 at 2173.726 kb on + strand, within Pf6N2E2_1835 at 2173.730 kb on + strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.783 kb on + strand, within Pf6N2E2_1835 at 2173.783 kb on + strand, within Pf6N2E2_1835 at 2173.784 kb on - strand, within Pf6N2E2_1835 at 2173.927 kb on + strand, within Pf6N2E2_1835 at 2174.131 kb on + strand at 2174.132 kb on - strand at 2174.132 kb on - strand at 2174.340 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,171,926 + Pf6N2E2_1833 0.51 +0.9 2,171,926 + Pf6N2E2_1833 0.51 +0.0 2,172,079 + Pf6N2E2_1833 0.61 -0.1 2,172,081 + Pf6N2E2_1833 0.61 +0.0 2,172,156 + Pf6N2E2_1833 0.66 -0.7 2,172,156 + Pf6N2E2_1833 0.66 +1.0 2,172,317 + Pf6N2E2_1833 0.77 +0.1 2,172,317 + Pf6N2E2_1833 0.77 +1.2 2,172,318 - Pf6N2E2_1833 0.77 -0.3 2,172,318 - Pf6N2E2_1833 0.77 +0.8 2,172,506 + Pf6N2E2_1833 0.89 -1.3 2,172,574 + +0.0 2,172,575 - -1.6 2,172,575 - +0.1 2,172,663 - -0.6 2,172,668 + +2.2 2,172,668 + -0.7 2,172,669 - -0.5 2,172,669 - -0.3 2,172,672 + +0.2 2,172,673 - +0.4 2,173,007 + Pf6N2E2_1834 0.44 -0.2 2,173,239 - Pf6N2E2_1834 0.74 -1.1 2,173,399 + -0.2 2,173,442 - -0.2 2,173,442 - -1.3 2,173,448 + +0.5 2,173,675 + Pf6N2E2_1835 0.39 -0.5 2,173,676 - Pf6N2E2_1835 0.39 -1.3 2,173,726 + Pf6N2E2_1835 0.47 +0.8 2,173,730 + Pf6N2E2_1835 0.48 -0.2 2,173,731 - Pf6N2E2_1835 0.48 -0.7 2,173,731 - Pf6N2E2_1835 0.48 -0.2 2,173,731 - Pf6N2E2_1835 0.48 -0.4 2,173,783 + Pf6N2E2_1835 0.56 -0.0 2,173,783 + Pf6N2E2_1835 0.56 -2.1 2,173,784 - Pf6N2E2_1835 0.57 -0.5 2,173,927 + Pf6N2E2_1835 0.80 -0.3 2,174,131 + +0.8 2,174,132 - +0.4 2,174,132 - -0.3 2,174,340 + -0.3
Or see this region's nucleotide sequence