Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_114

Experiment: LB no stress 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_113 and Pf6N2E2_114 are separated by 104 nucleotidesPf6N2E2_114 and Pf6N2E2_115 overlap by 4 nucleotides Pf6N2E2_113 - FIG00963512: hypothetical protein, at 158,312 to 160,225 _113 Pf6N2E2_114 - Polysaccharide biosynthesis protein, at 160,330 to 161,634 _114 Pf6N2E2_115 - FIG00958408: hypothetical protein, at 161,631 to 162,635 _115 Position (kb) 160 161 162Strain fitness (log2 ratio) -1 0 1 2at 159.441 kb on + strand, within Pf6N2E2_113at 159.441 kb on + strand, within Pf6N2E2_113at 159.441 kb on + strand, within Pf6N2E2_113at 159.444 kb on + strand, within Pf6N2E2_113at 159.444 kb on + strand, within Pf6N2E2_113at 159.444 kb on + strand, within Pf6N2E2_113at 159.444 kb on + strand, within Pf6N2E2_113at 159.445 kb on - strand, within Pf6N2E2_113at 159.445 kb on - strand, within Pf6N2E2_113at 159.445 kb on - strand, within Pf6N2E2_113at 159.507 kb on + strand, within Pf6N2E2_113at 159.521 kb on - strand, within Pf6N2E2_113at 159.735 kb on + strand, within Pf6N2E2_113at 159.864 kb on + strand, within Pf6N2E2_113at 159.864 kb on + strand, within Pf6N2E2_113at 159.865 kb on - strand, within Pf6N2E2_113at 159.865 kb on - strand, within Pf6N2E2_113at 159.865 kb on - strand, within Pf6N2E2_113at 159.865 kb on - strand, within Pf6N2E2_113at 159.955 kb on - strand, within Pf6N2E2_113at 160.093 kb on - strandat 160.150 kb on - strandat 160.173 kb on + strandat 160.173 kb on + strandat 160.173 kb on + strandat 160.297 kb on + strandat 160.387 kb on - strandat 160.427 kb on + strandat 160.428 kb on - strandat 160.460 kb on + strandat 160.461 kb on - strand, within Pf6N2E2_114at 160.461 kb on - strand, within Pf6N2E2_114at 161.390 kb on - strand, within Pf6N2E2_114at 161.618 kb on + strandat 161.741 kb on - strand, within Pf6N2E2_115at 161.828 kb on - strand, within Pf6N2E2_115at 161.833 kb on + strand, within Pf6N2E2_115at 161.833 kb on + strand, within Pf6N2E2_115at 161.833 kb on + strand, within Pf6N2E2_115at 161.833 kb on + strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 161.834 kb on - strand, within Pf6N2E2_115at 162.085 kb on + strand, within Pf6N2E2_115at 162.086 kb on - strand, within Pf6N2E2_115at 162.086 kb on - strandat 162.278 kb on + strand, within Pf6N2E2_115

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 24 hours
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159,441 + Pf6N2E2_113 0.59 -0.6
159,441 + Pf6N2E2_113 0.59 -0.2
159,441 + Pf6N2E2_113 0.59 -0.9
159,444 + Pf6N2E2_113 0.59 -0.2
159,444 + Pf6N2E2_113 0.59 -1.3
159,444 + Pf6N2E2_113 0.59 -0.2
159,444 + Pf6N2E2_113 0.59 +0.0
159,445 - Pf6N2E2_113 0.59 +0.2
159,445 - Pf6N2E2_113 0.59 +0.0
159,445 - Pf6N2E2_113 0.59 -1.2
159,507 + Pf6N2E2_113 0.62 -0.2
159,521 - Pf6N2E2_113 0.63 +0.2
159,735 + Pf6N2E2_113 0.74 +0.9
159,864 + Pf6N2E2_113 0.81 -0.0
159,864 + Pf6N2E2_113 0.81 -0.1
159,865 - Pf6N2E2_113 0.81 -0.8
159,865 - Pf6N2E2_113 0.81 -0.3
159,865 - Pf6N2E2_113 0.81 +0.7
159,865 - Pf6N2E2_113 0.81 -0.8
159,955 - Pf6N2E2_113 0.86 +0.6
160,093 - -0.2
160,150 - +0.5
160,173 + +1.8
160,173 + -1.3
160,173 + +0.3
160,297 + +0.3
160,387 - -0.2
160,427 + +1.4
160,428 - +1.0
160,460 + -0.2
160,461 - Pf6N2E2_114 0.10 -0.1
160,461 - Pf6N2E2_114 0.10 -1.2
161,390 - Pf6N2E2_114 0.81 -1.0
161,618 + +0.3
161,741 - Pf6N2E2_115 0.11 -0.7
161,828 - Pf6N2E2_115 0.20 +0.5
161,833 + Pf6N2E2_115 0.20 -1.1
161,833 + Pf6N2E2_115 0.20 -0.2
161,833 + Pf6N2E2_115 0.20 +1.2
161,833 + Pf6N2E2_115 0.20 +1.8
161,834 - Pf6N2E2_115 0.20 +1.0
161,834 - Pf6N2E2_115 0.20 +0.7
161,834 - Pf6N2E2_115 0.20 +1.0
161,834 - Pf6N2E2_115 0.20 -0.0
161,834 - Pf6N2E2_115 0.20 -1.3
161,834 - Pf6N2E2_115 0.20 +0.9
161,834 - Pf6N2E2_115 0.20 +0.6
161,834 - Pf6N2E2_115 0.20 -0.3
161,834 - Pf6N2E2_115 0.20 +0.1
162,085 + Pf6N2E2_115 0.45 -0.3
162,086 - Pf6N2E2_115 0.45 -0.2
162,086 - +2.4
162,278 + Pf6N2E2_115 0.64 +1.2

Or see this region's nucleotide sequence