Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_717 and Pf1N1B4_718 are separated by 14 nucleotides Pf1N1B4_718 and Pf1N1B4_719 are separated by 225 nucleotides
Pf1N1B4_717 - Iron-regulated protein A precursor, at 817,703 to 818,767
_717
Pf1N1B4_718 - outer membrane component of uptake system, probably for ferrous iron (from data) , at 818,782 to 819,882
_718
Pf1N1B4_719 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 820,108 to 821,256
_719
Position (kb)
818
819
820 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 817.823 kb on + strand, within Pf1N1B4_717 at 817.824 kb on - strand, within Pf1N1B4_717 at 818.101 kb on - strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.580 kb on - strand, within Pf1N1B4_717 at 818.672 kb on + strand at 818.672 kb on + strand at 818.672 kb on + strand at 818.672 kb on + strand at 818.918 kb on - strand, within Pf1N1B4_718 at 819.011 kb on + strand, within Pf1N1B4_718 at 819.050 kb on - strand, within Pf1N1B4_718 at 819.115 kb on + strand, within Pf1N1B4_718 at 819.115 kb on + strand, within Pf1N1B4_718 at 819.154 kb on + strand, within Pf1N1B4_718 at 819.155 kb on - strand, within Pf1N1B4_718 at 819.205 kb on + strand, within Pf1N1B4_718 at 819.205 kb on + strand, within Pf1N1B4_718 at 819.206 kb on - strand, within Pf1N1B4_718 at 819.206 kb on - strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.386 kb on + strand, within Pf1N1B4_718 at 819.604 kb on + strand, within Pf1N1B4_718 at 819.604 kb on + strand, within Pf1N1B4_718 at 819.605 kb on - strand, within Pf1N1B4_718 at 819.678 kb on + strand, within Pf1N1B4_718 at 819.679 kb on - strand, within Pf1N1B4_718 at 819.679 kb on - strand, within Pf1N1B4_718 at 819.704 kb on + strand, within Pf1N1B4_718 at 819.705 kb on - strand, within Pf1N1B4_718 at 819.794 kb on - strand at 819.796 kb on + strand at 819.797 kb on - strand at 819.797 kb on - strand at 819.797 kb on - strand at 819.839 kb on + strand at 819.881 kb on - strand at 819.907 kb on - strand at 819.954 kb on - strand at 819.972 kb on + strand at 819.972 kb on + strand at 819.973 kb on - strand at 820.032 kb on + strand at 820.032 kb on + strand at 820.033 kb on - strand at 820.061 kb on + strand at 820.061 kb on + strand at 820.107 kb on + strand at 820.107 kb on + strand at 820.107 kb on + strand at 820.107 kb on + strand at 820.107 kb on + strand at 820.108 kb on - strand at 820.108 kb on - strand at 820.108 kb on - strand at 820.108 kb on - strand at 820.108 kb on - strand at 820.151 kb on + strand at 820.174 kb on + strand at 820.192 kb on + strand at 820.192 kb on + strand at 820.192 kb on + strand at 820.192 kb on + strand at 820.192 kb on + strand at 820.192 kb on + strand at 820.193 kb on - strand at 820.193 kb on - strand at 820.193 kb on - strand at 820.232 kb on + strand, within Pf1N1B4_719 at 820.233 kb on - strand, within Pf1N1B4_719 at 820.233 kb on - strand, within Pf1N1B4_719 at 820.475 kb on - strand, within Pf1N1B4_719 at 820.657 kb on + strand, within Pf1N1B4_719 at 820.658 kb on - strand, within Pf1N1B4_719 at 820.708 kb on + strand, within Pf1N1B4_719 at 820.709 kb on - strand, within Pf1N1B4_719 at 820.709 kb on - strand, within Pf1N1B4_719 at 820.767 kb on - strand, within Pf1N1B4_719 at 820.813 kb on - strand, within Pf1N1B4_719
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 817,823 + Pf1N1B4_717 0.11 -2.0 817,824 - Pf1N1B4_717 0.11 +0.1 818,101 - Pf1N1B4_717 0.37 -1.0 818,129 + Pf1N1B4_717 0.40 -0.7 818,129 + Pf1N1B4_717 0.40 -0.9 818,129 + -0.1 818,129 + Pf1N1B4_717 0.40 -0.6 818,129 + Pf1N1B4_717 0.40 -1.3 818,129 + +1.1 818,129 + Pf1N1B4_717 0.40 -1.9 818,129 + Pf1N1B4_717 0.40 -1.1 818,129 + Pf1N1B4_717 0.40 -0.2 818,129 + Pf1N1B4_717 0.40 -1.8 818,130 - Pf1N1B4_717 0.40 +1.1 818,130 - Pf1N1B4_717 0.40 -1.0 818,130 - Pf1N1B4_717 0.40 -0.1 818,130 - Pf1N1B4_717 0.40 +0.1 818,130 - Pf1N1B4_717 0.40 -1.2 818,130 - Pf1N1B4_717 0.40 +0.4 818,130 - Pf1N1B4_717 0.40 +0.2 818,130 - Pf1N1B4_717 0.40 -0.9 818,183 + Pf1N1B4_717 0.45 -1.8 818,183 + Pf1N1B4_717 0.45 -1.3 818,183 + Pf1N1B4_717 0.45 -0.2 818,183 + Pf1N1B4_717 0.45 -0.9 818,184 - Pf1N1B4_717 0.45 -1.9 818,184 - Pf1N1B4_717 0.45 -2.2 818,184 - Pf1N1B4_717 0.45 +0.6 818,580 - Pf1N1B4_717 0.82 -1.2 818,672 + -1.9 818,672 + -1.0 818,672 + -1.3 818,672 + +0.1 818,918 - Pf1N1B4_718 0.12 -1.5 819,011 + Pf1N1B4_718 0.21 -1.9 819,050 - Pf1N1B4_718 0.24 +0.5 819,115 + Pf1N1B4_718 0.30 -1.9 819,115 + Pf1N1B4_718 0.30 -0.7 819,154 + Pf1N1B4_718 0.34 -1.5 819,155 - Pf1N1B4_718 0.34 -0.3 819,205 + Pf1N1B4_718 0.38 -2.3 819,205 + Pf1N1B4_718 0.38 +0.1 819,206 - Pf1N1B4_718 0.39 -1.0 819,206 - Pf1N1B4_718 0.39 +1.4 819,207 + Pf1N1B4_718 0.39 -0.8 819,207 + Pf1N1B4_718 0.39 -0.1 819,207 + Pf1N1B4_718 0.39 -0.7 819,207 + Pf1N1B4_718 0.39 -0.9 819,207 + Pf1N1B4_718 0.39 -0.9 819,207 + Pf1N1B4_718 0.39 -1.0 819,207 + Pf1N1B4_718 0.39 -0.2 819,208 - Pf1N1B4_718 0.39 -0.8 819,208 - Pf1N1B4_718 0.39 -1.4 819,208 - Pf1N1B4_718 0.39 -1.8 819,208 - Pf1N1B4_718 0.39 -2.9 819,386 + Pf1N1B4_718 0.55 -1.3 819,604 + Pf1N1B4_718 0.75 -0.8 819,604 + Pf1N1B4_718 0.75 -1.9 819,605 - Pf1N1B4_718 0.75 -0.6 819,678 + Pf1N1B4_718 0.81 +0.1 819,679 - Pf1N1B4_718 0.81 -0.5 819,679 - Pf1N1B4_718 0.81 -2.5 819,704 + Pf1N1B4_718 0.84 -1.6 819,705 - Pf1N1B4_718 0.84 -0.9 819,794 - -1.7 819,796 + +1.1 819,797 - -1.5 819,797 - -1.9 819,797 - -1.9 819,839 + -1.9 819,881 - -1.1 819,907 - +0.7 819,954 - +2.5 819,972 + +1.1 819,972 + +0.7 819,973 - +0.2 820,032 + +2.1 820,032 + +0.5 820,033 - -0.5 820,061 + +0.2 820,061 + +0.5 820,107 + +0.4 820,107 + -0.6 820,107 + +0.1 820,107 + +0.2 820,107 + +1.0 820,108 - -0.2 820,108 - -0.4 820,108 - -0.4 820,108 - +1.2 820,108 - +0.4 820,151 + +0.6 820,174 + +1.1 820,192 + +1.3 820,192 + -0.4 820,192 + -0.3 820,192 + +1.0 820,192 + +2.1 820,192 + -0.4 820,193 - -1.3 820,193 - -0.9 820,193 - -0.8 820,232 + Pf1N1B4_719 0.11 +0.5 820,233 - Pf1N1B4_719 0.11 +0.5 820,233 - Pf1N1B4_719 0.11 +1.1 820,475 - Pf1N1B4_719 0.32 +0.0 820,657 + Pf1N1B4_719 0.48 -0.8 820,658 - Pf1N1B4_719 0.48 -0.6 820,708 + Pf1N1B4_719 0.52 -0.5 820,709 - Pf1N1B4_719 0.52 +0.5 820,709 - Pf1N1B4_719 0.52 -0.8 820,767 - Pf1N1B4_719 0.57 -0.7 820,813 - Pf1N1B4_719 0.61 -1.6
Or see this region's nucleotide sequence