Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_714 and Pf1N1B4_715 are separated by 323 nucleotides Pf1N1B4_715 and Pf1N1B4_716 are separated by 261 nucleotides
Pf1N1B4_714 - GGDEF domain/EAL domain protein, at 812,271 to 814,319
_714
Pf1N1B4_715 - Iron-regulated protein A precursor, at 814,643 to 815,986
_715
Pf1N1B4_716 - Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites, at 816,248 to 817,675
_716
Position (kb)
814
815
816 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 813.832 kb on + strand, within Pf1N1B4_714 at 813.833 kb on - strand, within Pf1N1B4_714 at 813.833 kb on - strand, within Pf1N1B4_714 at 813.879 kb on + strand, within Pf1N1B4_714 at 813.879 kb on + strand, within Pf1N1B4_714 at 813.879 kb on + strand, within Pf1N1B4_714 at 813.879 kb on + strand, within Pf1N1B4_714 at 813.880 kb on - strand, within Pf1N1B4_714 at 813.880 kb on - strand, within Pf1N1B4_714 at 814.032 kb on + strand, within Pf1N1B4_714 at 814.033 kb on - strand, within Pf1N1B4_714 at 814.200 kb on + strand at 814.200 kb on + strand at 814.200 kb on + strand at 814.200 kb on + strand at 814.200 kb on + strand at 814.200 kb on + strand at 814.200 kb on + strand at 814.200 kb on + strand at 814.201 kb on - strand at 814.201 kb on - strand at 814.201 kb on - strand at 814.201 kb on - strand at 814.201 kb on - strand at 814.201 kb on - strand at 814.201 kb on - strand at 814.201 kb on - strand at 814.236 kb on + strand at 814.242 kb on + strand at 814.243 kb on - strand at 814.243 kb on - strand at 814.243 kb on - strand at 814.243 kb on - strand at 814.279 kb on - strand at 814.334 kb on + strand at 814.334 kb on + strand at 814.334 kb on + strand at 814.335 kb on - strand at 814.409 kb on - strand at 814.497 kb on + strand at 814.498 kb on - strand at 814.498 kb on - strand at 814.498 kb on - strand at 814.498 kb on - strand at 814.651 kb on + strand at 814.652 kb on - strand at 814.652 kb on - strand at 814.687 kb on + strand at 814.687 kb on + strand at 814.857 kb on - strand, within Pf1N1B4_715 at 814.977 kb on - strand at 815.071 kb on + strand, within Pf1N1B4_715 at 815.073 kb on - strand, within Pf1N1B4_715 at 815.087 kb on + strand, within Pf1N1B4_715 at 815.087 kb on + strand, within Pf1N1B4_715 at 815.088 kb on - strand, within Pf1N1B4_715 at 815.088 kb on - strand at 815.140 kb on + strand at 815.140 kb on + strand, within Pf1N1B4_715 at 815.378 kb on + strand, within Pf1N1B4_715 at 815.379 kb on - strand, within Pf1N1B4_715 at 815.431 kb on + strand, within Pf1N1B4_715 at 815.460 kb on - strand at 815.460 kb on - strand, within Pf1N1B4_715 at 815.637 kb on - strand, within Pf1N1B4_715 at 815.656 kb on + strand, within Pf1N1B4_715 at 815.663 kb on + strand, within Pf1N1B4_715 at 815.664 kb on - strand, within Pf1N1B4_715 at 815.675 kb on + strand, within Pf1N1B4_715 at 815.676 kb on - strand, within Pf1N1B4_715 at 815.831 kb on + strand, within Pf1N1B4_715 at 816.006 kb on + strand at 816.007 kb on - strand at 816.207 kb on - strand at 816.645 kb on - strand, within Pf1N1B4_716 at 816.740 kb on - strand, within Pf1N1B4_716
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 813,832 + Pf1N1B4_714 0.76 -0.2 813,833 - Pf1N1B4_714 0.76 +0.3 813,833 - Pf1N1B4_714 0.76 -1.0 813,879 + Pf1N1B4_714 0.78 -1.9 813,879 + Pf1N1B4_714 0.78 +1.5 813,879 + Pf1N1B4_714 0.78 +1.1 813,879 + Pf1N1B4_714 0.78 -0.6 813,880 - Pf1N1B4_714 0.79 -1.3 813,880 - Pf1N1B4_714 0.79 +0.1 814,032 + Pf1N1B4_714 0.86 -0.3 814,033 - Pf1N1B4_714 0.86 +0.2 814,200 + +0.8 814,200 + +1.3 814,200 + -0.1 814,200 + +0.5 814,200 + -1.5 814,200 + -1.9 814,200 + +2.1 814,200 + -0.3 814,201 - +0.4 814,201 - -0.3 814,201 - +1.4 814,201 - +0.7 814,201 - +0.7 814,201 - +1.7 814,201 - +0.5 814,201 - -0.7 814,236 + +0.4 814,242 + +0.1 814,243 - +0.1 814,243 - -0.0 814,243 - +0.5 814,243 - -0.8 814,279 - +0.3 814,334 + -0.9 814,334 + -1.5 814,334 + +1.5 814,335 - -0.5 814,409 - -0.3 814,497 + +0.1 814,498 - -0.9 814,498 - -0.1 814,498 - -0.7 814,498 - +0.2 814,651 + -1.0 814,652 - -1.4 814,652 - -1.7 814,687 + -2.1 814,687 + -0.9 814,857 - Pf1N1B4_715 0.16 -1.5 814,977 - +2.1 815,071 + Pf1N1B4_715 0.32 -2.6 815,073 - Pf1N1B4_715 0.32 -1.6 815,087 + Pf1N1B4_715 0.33 +0.4 815,087 + Pf1N1B4_715 0.33 -2.2 815,088 - Pf1N1B4_715 0.33 -1.8 815,088 - -0.3 815,140 + -1.1 815,140 + Pf1N1B4_715 0.37 -1.0 815,378 + Pf1N1B4_715 0.55 -0.6 815,379 - Pf1N1B4_715 0.55 -0.2 815,431 + Pf1N1B4_715 0.59 -0.9 815,460 - -0.8 815,460 - Pf1N1B4_715 0.61 -0.6 815,637 - Pf1N1B4_715 0.74 -0.4 815,656 + Pf1N1B4_715 0.75 -0.9 815,663 + Pf1N1B4_715 0.76 -1.0 815,664 - Pf1N1B4_715 0.76 -1.9 815,675 + Pf1N1B4_715 0.77 -1.3 815,676 - Pf1N1B4_715 0.77 -0.8 815,831 + Pf1N1B4_715 0.88 -1.6 816,006 + -0.9 816,007 - +0.9 816,207 - -1.9 816,645 - Pf1N1B4_716 0.28 +0.5 816,740 - Pf1N1B4_716 0.34 -2.2
Or see this region's nucleotide sequence