Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_691 and Pf1N1B4_692 are separated by 15 nucleotides Pf1N1B4_692 and Pf1N1B4_693 overlap by 4 nucleotides Pf1N1B4_693 and Pf1N1B4_694 are separated by 293 nucleotides
Pf1N1B4_691 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 789,195 to 790,250
_691
Pf1N1B4_692 - ABC spermidine/putrescine transporter, inner membrane subunit, at 790,266 to 791,114
_692
Pf1N1B4_693 - ABC spermidine/putrescine transporter, inner membrane subunit, at 791,111 to 791,917
_693
Pf1N1B4_694 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 792,211 to 793,314
_694
Position (kb)
791
792 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.427 kb on - strand, within Pf1N1B4_692 at 790.427 kb on - strand, within Pf1N1B4_692 at 790.435 kb on + strand, within Pf1N1B4_692 at 790.436 kb on - strand, within Pf1N1B4_692 at 790.436 kb on - strand, within Pf1N1B4_692 at 790.798 kb on + strand, within Pf1N1B4_692 at 790.798 kb on + strand, within Pf1N1B4_692 at 790.799 kb on - strand at 790.799 kb on - strand, within Pf1N1B4_692 at 790.799 kb on - strand, within Pf1N1B4_692 at 790.799 kb on - strand, within Pf1N1B4_692 at 790.933 kb on + strand at 790.934 kb on - strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 791.154 kb on + strand at 791.154 kb on + strand at 791.155 kb on - strand at 791.155 kb on - strand at 791.155 kb on - strand at 791.433 kb on + strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.712 kb on + strand, within Pf1N1B4_693 at 791.712 kb on + strand, within Pf1N1B4_693 at 791.713 kb on - strand, within Pf1N1B4_693 at 791.793 kb on + strand at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand at 791.817 kb on + strand, within Pf1N1B4_693 at 791.877 kb on - strand at 792.113 kb on + strand at 792.113 kb on + strand at 792.187 kb on + strand at 792.187 kb on + strand at 792.188 kb on - strand at 792.188 kb on - strand at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand at 792.380 kb on + strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.441 kb on - strand, within Pf1N1B4_694 at 792.441 kb on - strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.501 kb on - strand, within Pf1N1B4_694 at 792.539 kb on + strand, within Pf1N1B4_694 at 792.540 kb on - strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.582 kb on - strand, within Pf1N1B4_694 at 792.666 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.855 kb on - strand, within Pf1N1B4_694
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 790,390 + Pf1N1B4_692 0.15 -2.1 790,390 + Pf1N1B4_692 0.15 -0.5 790,390 + Pf1N1B4_692 0.15 -0.3 790,390 + Pf1N1B4_692 0.15 +0.8 790,390 + Pf1N1B4_692 0.15 -1.0 790,390 + Pf1N1B4_692 0.15 -0.9 790,390 + Pf1N1B4_692 0.15 +0.0 790,391 - Pf1N1B4_692 0.15 +0.1 790,391 - Pf1N1B4_692 0.15 -0.6 790,391 - Pf1N1B4_692 0.15 +0.4 790,391 - Pf1N1B4_692 0.15 +0.8 790,427 - Pf1N1B4_692 0.19 -0.0 790,427 - Pf1N1B4_692 0.19 +0.6 790,435 + Pf1N1B4_692 0.20 -0.6 790,436 - Pf1N1B4_692 0.20 +0.7 790,436 - Pf1N1B4_692 0.20 +1.4 790,798 + Pf1N1B4_692 0.63 +0.2 790,798 + Pf1N1B4_692 0.63 -1.0 790,799 - +0.4 790,799 - Pf1N1B4_692 0.63 +0.9 790,799 - Pf1N1B4_692 0.63 +0.8 790,799 - Pf1N1B4_692 0.63 +0.3 790,933 + +0.2 790,934 - Pf1N1B4_692 0.79 -0.6 790,948 + Pf1N1B4_692 0.80 +0.3 790,948 + Pf1N1B4_692 0.80 +0.9 790,948 + Pf1N1B4_692 0.80 -0.6 790,949 - Pf1N1B4_692 0.80 +1.0 790,949 - Pf1N1B4_692 0.80 +1.3 790,949 - Pf1N1B4_692 0.80 -1.2 791,154 + -0.4 791,154 + -0.3 791,155 - -0.1 791,155 - -2.2 791,155 - -1.0 791,433 + Pf1N1B4_693 0.40 -1.5 791,530 - Pf1N1B4_693 0.52 -1.2 791,530 - Pf1N1B4_693 0.52 -1.4 791,530 - Pf1N1B4_693 0.52 -2.5 791,530 - Pf1N1B4_693 0.52 -0.3 791,712 + Pf1N1B4_693 0.74 -1.7 791,712 + Pf1N1B4_693 0.74 -1.2 791,713 - Pf1N1B4_693 0.75 +0.1 791,793 + -0.8 791,797 - Pf1N1B4_693 0.85 +0.5 791,797 - Pf1N1B4_693 0.85 -0.2 791,797 - Pf1N1B4_693 0.85 -0.1 791,797 - Pf1N1B4_693 0.85 -0.6 791,797 - Pf1N1B4_693 0.85 -1.4 791,797 - -1.0 791,817 + Pf1N1B4_693 0.87 +0.9 791,877 - +0.3 792,113 + -0.3 792,113 + -0.8 792,187 + -0.3 792,187 + +0.0 792,188 - -2.2 792,188 - +0.4 792,323 + Pf1N1B4_694 0.10 -0.1 792,323 + Pf1N1B4_694 0.10 +0.5 792,323 + Pf1N1B4_694 0.10 +0.8 792,323 + Pf1N1B4_694 0.10 -0.6 792,324 - Pf1N1B4_694 0.10 -0.3 792,324 - Pf1N1B4_694 0.10 +0.0 792,324 - Pf1N1B4_694 0.10 +2.0 792,324 - Pf1N1B4_694 0.10 -0.1 792,324 - Pf1N1B4_694 0.10 -0.2 792,380 + Pf1N1B4_694 0.15 +1.0 792,380 + Pf1N1B4_694 0.15 -0.6 792,380 + Pf1N1B4_694 0.15 +0.8 792,380 + Pf1N1B4_694 0.15 +0.0 792,380 + +0.6 792,380 + Pf1N1B4_694 0.15 -0.3 792,381 - Pf1N1B4_694 0.15 +0.0 792,381 - Pf1N1B4_694 0.15 +0.7 792,381 - Pf1N1B4_694 0.15 +0.6 792,441 - Pf1N1B4_694 0.21 +0.3 792,441 - Pf1N1B4_694 0.21 +0.3 792,500 + Pf1N1B4_694 0.26 +0.4 792,500 + Pf1N1B4_694 0.26 +0.2 792,500 + Pf1N1B4_694 0.26 -0.1 792,501 - Pf1N1B4_694 0.26 -0.1 792,539 + Pf1N1B4_694 0.30 -0.8 792,540 - Pf1N1B4_694 0.30 +0.0 792,581 + Pf1N1B4_694 0.34 +1.0 792,581 + Pf1N1B4_694 0.34 +0.5 792,582 - Pf1N1B4_694 0.34 -1.4 792,666 + Pf1N1B4_694 0.41 -1.6 792,818 + Pf1N1B4_694 0.55 +2.2 792,818 + Pf1N1B4_694 0.55 +0.1 792,818 + Pf1N1B4_694 0.55 -1.4 792,818 + Pf1N1B4_694 0.55 -1.7 792,819 - Pf1N1B4_694 0.55 +0.9 792,819 - Pf1N1B4_694 0.55 +0.1 792,855 - Pf1N1B4_694 0.58 +0.2
Or see this region's nucleotide sequence