Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5809 and Pf1N1B4_5810 are separated by 247 nucleotides Pf1N1B4_5810 and Pf1N1B4_5811 are separated by 67 nucleotides Pf1N1B4_5811 and Pf1N1B4_5812 overlap by 4 nucleotides
Pf1N1B4_5809 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 243,057 to 244,004
_5809
Pf1N1B4_5810 - hypothetical protein, at 244,252 to 244,410
_5810
Pf1N1B4_5811 - FIG00958502: hypothetical protein, at 244,478 to 244,792
_5811
Pf1N1B4_5812 - FIG00955248: hypothetical protein, at 244,789 to 245,487
_5812
Position (kb) 244
245 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 243.541 kb on + strand, within Pf1N1B4_5809 at 243.610 kb on + strand, within Pf1N1B4_5809 at 243.610 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.844 kb on + strand, within Pf1N1B4_5809 at 243.895 kb on + strand, within Pf1N1B4_5809 at 243.898 kb on + strand, within Pf1N1B4_5809 at 243.940 kb on + strand at 243.988 kb on + strand at 244.144 kb on + strand at 244.176 kb on + strand at 244.176 kb on + strand at 244.177 kb on - strand at 244.222 kb on - strand at 244.454 kb on + strand at 244.454 kb on + strand at 244.454 kb on + strand at 244.455 kb on - strand at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.549 kb on - strand at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.733 kb on + strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.805 kb on + strand at 244.805 kb on + strand at 244.806 kb on - strand at 244.806 kb on - strand at 244.822 kb on - strand at 245.067 kb on - strand, within Pf1N1B4_5812 at 245.094 kb on - strand, within Pf1N1B4_5812 at 245.094 kb on - strand, within Pf1N1B4_5812 at 245.211 kb on + strand, within Pf1N1B4_5812 at 245.212 kb on - strand, within Pf1N1B4_5812 at 245.212 kb on - strand, within Pf1N1B4_5812 at 245.212 kb on - strand, within Pf1N1B4_5812 at 245.212 kb on - strand, within Pf1N1B4_5812 at 245.487 kb on + strand at 245.488 kb on - strand at 245.496 kb on - strand at 245.615 kb on - strand at 245.640 kb on + strand at 245.641 kb on - strand at 245.641 kb on - strand at 245.641 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 243,541 + Pf1N1B4_5809 0.51 -0.3 243,610 + Pf1N1B4_5809 0.58 +0.9 243,610 + Pf1N1B4_5809 0.58 +0.0 243,630 + Pf1N1B4_5809 0.60 +1.9 243,630 + Pf1N1B4_5809 0.60 -0.2 243,630 + Pf1N1B4_5809 0.60 -0.3 243,844 + Pf1N1B4_5809 0.83 -0.2 243,895 + Pf1N1B4_5809 0.88 +0.0 243,898 + Pf1N1B4_5809 0.89 +0.2 243,940 + +0.4 243,988 + +0.0 244,144 + +0.0 244,176 + -0.4 244,176 + -0.4 244,177 - +0.4 244,222 - -1.1 244,454 + -0.3 244,454 + -1.2 244,454 + -0.3 244,455 - +0.6 244,548 + Pf1N1B4_5811 0.22 -0.8 244,548 + Pf1N1B4_5811 0.22 -0.8 244,548 + Pf1N1B4_5811 0.22 -0.3 244,548 + Pf1N1B4_5811 0.22 -1.7 244,548 + Pf1N1B4_5811 0.22 -0.8 244,549 - -1.5 244,549 - Pf1N1B4_5811 0.23 -0.8 244,549 - Pf1N1B4_5811 0.23 +0.2 244,549 - Pf1N1B4_5811 0.23 -0.5 244,549 - Pf1N1B4_5811 0.23 -1.9 244,733 + Pf1N1B4_5811 0.81 -0.9 244,734 - Pf1N1B4_5811 0.81 -2.8 244,734 - Pf1N1B4_5811 0.81 -1.1 244,734 - Pf1N1B4_5811 0.81 -0.0 244,734 - Pf1N1B4_5811 0.81 +0.3 244,734 - Pf1N1B4_5811 0.81 -2.3 244,805 + +0.4 244,805 + -0.5 244,806 - -0.2 244,806 - -0.1 244,822 - -0.7 245,067 - Pf1N1B4_5812 0.40 -0.3 245,094 - Pf1N1B4_5812 0.44 +1.5 245,094 - Pf1N1B4_5812 0.44 +0.4 245,211 + Pf1N1B4_5812 0.60 -1.5 245,212 - Pf1N1B4_5812 0.61 -1.3 245,212 - Pf1N1B4_5812 0.61 +1.0 245,212 - Pf1N1B4_5812 0.61 -0.8 245,212 - Pf1N1B4_5812 0.61 -1.4 245,487 + -0.0 245,488 - +0.0 245,496 - +0.3 245,615 - -2.1 245,640 + -0.5 245,641 - +0.6 245,641 - +0.6 245,641 - +0.3
Or see this region's nucleotide sequence