Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5490 and Pf1N1B4_5491 are separated by 335 nucleotides Pf1N1B4_5491 and Pf1N1B4_5492 are separated by 34 nucleotides Pf1N1B4_5492 and Pf1N1B4_5493 are separated by 91 nucleotides Pf1N1B4_5493 and Pf1N1B4_5494 are separated by 96 nucleotides
Pf1N1B4_5490 - hypothetical protein, at 237,805 to 238,038
_5490
Pf1N1B4_5491 - hypothetical protein, at 238,374 to 238,607
_5491
Pf1N1B4_5492 - hypothetical protein, at 238,642 to 238,875
_5492
Pf1N1B4_5493 - hypothetical protein, at 238,967 to 239,542
_5493
Pf1N1B4_5494 - Helicase IV (EC 3.6.1.-), at 239,639 to 242,071
_5494
Position (kb) 238
239 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 237.647 kb on - strand at 237.663 kb on + strand at 237.663 kb on + strand at 237.663 kb on + strand at 237.663 kb on + strand at 237.664 kb on - strand at 237.664 kb on - strand at 237.664 kb on - strand at 237.664 kb on - strand at 237.710 kb on + strand at 237.710 kb on + strand at 237.710 kb on + strand at 237.711 kb on - strand at 237.711 kb on - strand at 237.711 kb on - strand at 237.711 kb on - strand at 237.722 kb on + strand at 237.722 kb on + strand at 237.723 kb on - strand at 237.723 kb on - strand at 237.742 kb on + strand at 237.742 kb on + strand at 237.742 kb on + strand at 237.743 kb on - strand at 237.743 kb on - strand at 237.762 kb on - strand at 237.842 kb on + strand at 237.842 kb on + strand, within Pf1N1B4_5490 at 237.842 kb on + strand, within Pf1N1B4_5490 at 237.843 kb on - strand, within Pf1N1B4_5490 at 237.843 kb on - strand, within Pf1N1B4_5490 at 237.843 kb on - strand, within Pf1N1B4_5490 at 237.848 kb on + strand, within Pf1N1B4_5490 at 237.848 kb on + strand, within Pf1N1B4_5490 at 237.848 kb on + strand, within Pf1N1B4_5490 at 237.849 kb on - strand, within Pf1N1B4_5490 at 237.849 kb on - strand, within Pf1N1B4_5490 at 237.854 kb on + strand at 237.855 kb on - strand, within Pf1N1B4_5490 at 237.896 kb on + strand, within Pf1N1B4_5490 at 237.896 kb on + strand, within Pf1N1B4_5490 at 237.908 kb on + strand, within Pf1N1B4_5490 at 237.909 kb on - strand, within Pf1N1B4_5490 at 237.909 kb on - strand, within Pf1N1B4_5490 at 237.942 kb on + strand, within Pf1N1B4_5490 at 237.943 kb on - strand, within Pf1N1B4_5490 at 238.020 kb on + strand at 238.020 kb on + strand at 238.020 kb on + strand at 238.020 kb on + strand at 238.020 kb on + strand at 238.021 kb on - strand at 238.036 kb on + strand at 238.036 kb on + strand at 238.036 kb on + strand at 238.036 kb on + strand at 238.036 kb on + strand at 238.074 kb on + strand at 238.074 kb on + strand at 238.074 kb on + strand at 238.075 kb on - strand at 238.075 kb on - strand at 238.075 kb on - strand at 238.105 kb on + strand at 238.171 kb on + strand at 238.234 kb on + strand at 238.281 kb on + strand at 238.281 kb on + strand at 238.281 kb on + strand at 238.281 kb on + strand at 238.282 kb on - strand at 238.282 kb on - strand at 238.282 kb on - strand at 238.332 kb on + strand at 238.332 kb on + strand at 238.333 kb on - strand at 238.333 kb on - strand at 238.346 kb on + strand at 238.618 kb on + strand at 238.619 kb on - strand at 238.619 kb on - strand at 238.828 kb on + strand, within Pf1N1B4_5492 at 238.828 kb on + strand, within Pf1N1B4_5492 at 238.828 kb on + strand, within Pf1N1B4_5492 at 238.829 kb on - strand at 238.832 kb on + strand, within Pf1N1B4_5492 at 238.832 kb on + strand, within Pf1N1B4_5492 at 238.832 kb on + strand, within Pf1N1B4_5492 at 238.832 kb on + strand at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.833 kb on - strand at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.932 kb on + strand at 238.932 kb on + strand at 238.932 kb on + strand at 238.950 kb on + strand at 238.950 kb on + strand at 238.950 kb on + strand at 238.950 kb on + strand at 238.951 kb on - strand at 238.951 kb on - strand at 238.951 kb on - strand at 239.040 kb on + strand, within Pf1N1B4_5493 at 239.040 kb on + strand, within Pf1N1B4_5493 at 239.040 kb on + strand, within Pf1N1B4_5493 at 239.041 kb on - strand, within Pf1N1B4_5493 at 239.072 kb on + strand, within Pf1N1B4_5493 at 239.073 kb on - strand, within Pf1N1B4_5493 at 239.082 kb on + strand, within Pf1N1B4_5493 at 239.168 kb on - strand, within Pf1N1B4_5493 at 239.223 kb on - strand, within Pf1N1B4_5493 at 239.223 kb on - strand, within Pf1N1B4_5493 at 239.273 kb on + strand, within Pf1N1B4_5493 at 239.273 kb on + strand, within Pf1N1B4_5493 at 239.274 kb on - strand, within Pf1N1B4_5493 at 239.274 kb on - strand, within Pf1N1B4_5493 at 239.274 kb on - strand, within Pf1N1B4_5493 at 239.274 kb on - strand, within Pf1N1B4_5493 at 239.274 kb on - strand, within Pf1N1B4_5493 at 239.274 kb on - strand, within Pf1N1B4_5493 at 239.352 kb on - strand, within Pf1N1B4_5493 at 239.435 kb on + strand, within Pf1N1B4_5493 at 239.436 kb on - strand, within Pf1N1B4_5493 at 239.436 kb on - strand, within Pf1N1B4_5493 at 239.436 kb on - strand, within Pf1N1B4_5493 at 239.436 kb on - strand, within Pf1N1B4_5493 at 239.533 kb on + strand at 239.739 kb on + strand at 239.740 kb on - strand at 239.761 kb on + strand at 239.761 kb on + strand at 239.761 kb on + strand at 239.761 kb on + strand at 239.762 kb on - strand at 239.762 kb on - strand at 239.762 kb on - strand at 239.762 kb on - strand at 239.822 kb on - strand at 239.866 kb on + strand at 239.867 kb on - strand at 239.867 kb on - strand at 239.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 237,647 - +0.0 237,663 + -0.5 237,663 + -2.3 237,663 + -1.1 237,663 + +0.8 237,664 - -1.5 237,664 - +1.6 237,664 - -1.4 237,664 - -0.2 237,710 + -0.6 237,710 + +0.3 237,710 + +0.7 237,711 - -0.8 237,711 - +0.0 237,711 - +0.6 237,711 - +0.2 237,722 + -0.8 237,722 + -0.1 237,723 - +1.1 237,723 - +0.7 237,742 + -0.2 237,742 + +0.1 237,742 + +0.5 237,743 - +0.2 237,743 - -0.2 237,762 - -0.2 237,842 + -0.5 237,842 + Pf1N1B4_5490 0.16 +0.3 237,842 + Pf1N1B4_5490 0.16 -1.2 237,843 - Pf1N1B4_5490 0.16 -1.5 237,843 - Pf1N1B4_5490 0.16 -0.6 237,843 - Pf1N1B4_5490 0.16 +0.5 237,848 + Pf1N1B4_5490 0.18 +0.5 237,848 + Pf1N1B4_5490 0.18 +0.6 237,848 + Pf1N1B4_5490 0.18 -0.1 237,849 - Pf1N1B4_5490 0.19 +0.8 237,849 - Pf1N1B4_5490 0.19 -0.2 237,854 + +0.8 237,855 - Pf1N1B4_5490 0.21 +1.6 237,896 + Pf1N1B4_5490 0.39 -0.4 237,896 + Pf1N1B4_5490 0.39 +1.1 237,908 + Pf1N1B4_5490 0.44 +0.8 237,909 - Pf1N1B4_5490 0.44 +1.3 237,909 - Pf1N1B4_5490 0.44 +0.1 237,942 + Pf1N1B4_5490 0.59 +1.2 237,943 - Pf1N1B4_5490 0.59 -0.2 238,020 + -0.8 238,020 + -2.3 238,020 + -0.2 238,020 + -0.2 238,020 + -1.3 238,021 - -0.6 238,036 + -0.2 238,036 + -1.6 238,036 + +0.4 238,036 + -0.5 238,036 + -1.6 238,074 + -0.2 238,074 + -0.5 238,074 + -1.2 238,075 - -1.3 238,075 - -0.2 238,075 - +1.2 238,105 + +0.6 238,171 + -1.1 238,234 + -0.0 238,281 + -0.9 238,281 + -0.2 238,281 + +0.6 238,281 + +0.8 238,282 - -0.9 238,282 - +0.8 238,282 - +0.0 238,332 + +1.0 238,332 + +0.5 238,333 - -1.1 238,333 - +0.5 238,346 + -1.8 238,618 + +1.0 238,619 - -0.1 238,619 - -0.5 238,828 + Pf1N1B4_5492 0.79 -0.8 238,828 + Pf1N1B4_5492 0.79 -1.0 238,828 + Pf1N1B4_5492 0.79 -2.0 238,829 - -0.6 238,832 + Pf1N1B4_5492 0.81 -2.6 238,832 + Pf1N1B4_5492 0.81 -0.7 238,832 + Pf1N1B4_5492 0.81 -1.9 238,832 + +1.2 238,833 - Pf1N1B4_5492 0.82 -1.9 238,833 - Pf1N1B4_5492 0.82 -0.3 238,833 - +0.5 238,833 - Pf1N1B4_5492 0.82 -0.7 238,833 - Pf1N1B4_5492 0.82 -0.1 238,833 - Pf1N1B4_5492 0.82 -1.4 238,932 + +1.6 238,932 + -0.1 238,932 + +1.0 238,950 + -0.1 238,950 + -0.5 238,950 + -0.3 238,950 + +0.3 238,951 - -0.0 238,951 - +0.0 238,951 - -1.3 239,040 + Pf1N1B4_5493 0.13 -0.6 239,040 + Pf1N1B4_5493 0.13 -0.5 239,040 + Pf1N1B4_5493 0.13 +0.4 239,041 - Pf1N1B4_5493 0.13 +0.2 239,072 + Pf1N1B4_5493 0.18 +0.9 239,073 - Pf1N1B4_5493 0.18 +0.1 239,082 + Pf1N1B4_5493 0.20 -0.1 239,168 - Pf1N1B4_5493 0.35 -0.9 239,223 - Pf1N1B4_5493 0.44 -1.2 239,223 - Pf1N1B4_5493 0.44 +0.4 239,273 + Pf1N1B4_5493 0.53 -0.2 239,273 + Pf1N1B4_5493 0.53 +0.3 239,274 - Pf1N1B4_5493 0.53 -0.8 239,274 - Pf1N1B4_5493 0.53 -0.7 239,274 - Pf1N1B4_5493 0.53 -0.1 239,274 - Pf1N1B4_5493 0.53 -0.4 239,274 - Pf1N1B4_5493 0.53 +0.8 239,274 - Pf1N1B4_5493 0.53 -2.9 239,352 - Pf1N1B4_5493 0.67 +0.6 239,435 + Pf1N1B4_5493 0.81 +0.1 239,436 - Pf1N1B4_5493 0.81 +1.4 239,436 - Pf1N1B4_5493 0.81 +0.8 239,436 - Pf1N1B4_5493 0.81 -0.5 239,436 - Pf1N1B4_5493 0.81 -1.2 239,533 + +0.5 239,739 + +0.4 239,740 - -1.3 239,761 + -0.8 239,761 + -1.2 239,761 + -0.4 239,761 + -0.4 239,762 - +0.2 239,762 - +1.1 239,762 - -0.0 239,762 - -0.7 239,822 - -0.1 239,866 + -1.4 239,867 - +1.6 239,867 - -0.2 239,867 - +2.2
Or see this region's nucleotide sequence