Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5347 and Pf1N1B4_5348 are separated by 101 nucleotides Pf1N1B4_5348 and Pf1N1B4_5349 are separated by 741 nucleotides Pf1N1B4_5349 and Pf1N1B4_5350 are separated by 316 nucleotides
Pf1N1B4_5347 - hypothetical protein, at 88,005 to 88,352
_5347
Pf1N1B4_5348 - hypothetical protein, at 88,454 to 88,576
_5348
Pf1N1B4_5349 - hypothetical protein, at 89,318 to 89,953
_5349
Pf1N1B4_5350 - hypothetical protein, at 90,270 to 91,034
_5350
Position (kb)
89
90 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.560 kb on - strand at 88.560 kb on - strand, within Pf1N1B4_5348 at 88.642 kb on - strand at 88.671 kb on + strand at 88.672 kb on - strand at 88.793 kb on - strand at 88.803 kb on + strand at 88.804 kb on - strand at 88.859 kb on - strand at 88.874 kb on + strand at 88.874 kb on + strand at 88.875 kb on - strand at 88.875 kb on - strand at 88.893 kb on + strand at 88.970 kb on + strand at 88.970 kb on + strand at 88.970 kb on + strand at 88.970 kb on + strand at 88.971 kb on - strand at 88.971 kb on - strand at 88.971 kb on - strand at 88.976 kb on + strand at 88.976 kb on + strand at 88.976 kb on + strand at 88.976 kb on + strand at 88.977 kb on - strand at 89.028 kb on - strand at 89.028 kb on - strand at 89.270 kb on + strand at 89.270 kb on + strand at 89.271 kb on - strand at 89.271 kb on - strand at 89.300 kb on - strand at 89.390 kb on - strand, within Pf1N1B4_5349 at 89.390 kb on - strand, within Pf1N1B4_5349 at 89.390 kb on - strand at 89.506 kb on - strand, within Pf1N1B4_5349 at 89.506 kb on - strand, within Pf1N1B4_5349 at 89.534 kb on + strand, within Pf1N1B4_5349 at 89.637 kb on + strand, within Pf1N1B4_5349 at 89.637 kb on + strand, within Pf1N1B4_5349 at 89.638 kb on - strand, within Pf1N1B4_5349 at 89.679 kb on + strand at 89.680 kb on - strand, within Pf1N1B4_5349 at 89.908 kb on + strand at 89.909 kb on - strand at 89.909 kb on - strand at 90.148 kb on - strand at 90.197 kb on + strand at 90.197 kb on + strand at 90.197 kb on + strand at 90.222 kb on + strand at 90.223 kb on - strand at 90.242 kb on + strand at 90.457 kb on + strand, within Pf1N1B4_5350 at 90.457 kb on + strand, within Pf1N1B4_5350 at 90.458 kb on - strand, within Pf1N1B4_5350 at 90.458 kb on - strand, within Pf1N1B4_5350 at 90.677 kb on - strand, within Pf1N1B4_5350 at 90.730 kb on - strand, within Pf1N1B4_5350 at 90.868 kb on + strand, within Pf1N1B4_5350 at 90.869 kb on - strand, within Pf1N1B4_5350 at 90.916 kb on + strand, within Pf1N1B4_5350 at 90.916 kb on + strand, within Pf1N1B4_5350 at 90.917 kb on - strand, within Pf1N1B4_5350
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 88,495 - Pf1N1B4_5348 0.33 -2.1 88,495 - Pf1N1B4_5348 0.33 -0.0 88,495 - Pf1N1B4_5348 0.33 +0.3 88,560 - +0.4 88,560 - Pf1N1B4_5348 0.86 -0.7 88,642 - -0.7 88,671 + -1.0 88,672 - +0.4 88,793 - -0.4 88,803 + -0.1 88,804 - +0.0 88,859 - +1.4 88,874 + +0.8 88,874 + +0.1 88,875 - -2.3 88,875 - -1.3 88,893 + -0.6 88,970 + +0.1 88,970 + -0.5 88,970 + +0.7 88,970 + -0.3 88,971 - +0.5 88,971 - +0.8 88,971 - +0.2 88,976 + -0.3 88,976 + -0.9 88,976 + -0.6 88,976 + +0.0 88,977 - -0.5 89,028 - -0.8 89,028 - +0.4 89,270 + +0.8 89,270 + +0.5 89,271 - -2.4 89,271 - +0.6 89,300 - +0.8 89,390 - Pf1N1B4_5349 0.11 -0.3 89,390 - Pf1N1B4_5349 0.11 -0.8 89,390 - -0.2 89,506 - Pf1N1B4_5349 0.30 -1.9 89,506 - Pf1N1B4_5349 0.30 -1.6 89,534 + Pf1N1B4_5349 0.34 -0.6 89,637 + Pf1N1B4_5349 0.50 -0.2 89,637 + Pf1N1B4_5349 0.50 +0.3 89,638 - Pf1N1B4_5349 0.50 -1.3 89,679 + +0.6 89,680 - Pf1N1B4_5349 0.57 +0.1 89,908 + +0.6 89,909 - -1.3 89,909 - -0.2 90,148 - -0.6 90,197 + -1.2 90,197 + -0.6 90,197 + -1.6 90,222 + -0.2 90,223 - +1.8 90,242 + -1.3 90,457 + Pf1N1B4_5350 0.24 -0.9 90,457 + Pf1N1B4_5350 0.24 +0.4 90,458 - Pf1N1B4_5350 0.25 +0.5 90,458 - Pf1N1B4_5350 0.25 -0.6 90,677 - Pf1N1B4_5350 0.53 +1.1 90,730 - Pf1N1B4_5350 0.60 +1.5 90,868 + Pf1N1B4_5350 0.78 +0.5 90,869 - Pf1N1B4_5350 0.78 -0.2 90,916 + Pf1N1B4_5350 0.84 +1.5 90,916 + Pf1N1B4_5350 0.84 +1.2 90,917 - Pf1N1B4_5350 0.85 -0.7
Or see this region's nucleotide sequence