Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4948 and Pf1N1B4_4949 overlap by 4 nucleotides Pf1N1B4_4949 and Pf1N1B4_4950 are separated by 4 nucleotides Pf1N1B4_4950 and Pf1N1B4_4951 are separated by 140 nucleotides
Pf1N1B4_4948 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 5,485,045 to 5,485,989
_4948
Pf1N1B4_4949 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 5,485,986 to 5,486,639
_4949
Pf1N1B4_4950 - formate dehydrogenase formation protein FdhE, at 5,486,644 to 5,487,570
_4950
Pf1N1B4_4951 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 5,487,711 to 5,488,073
_4951
Position (kb)
5486
5487
5488 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5485.670 kb on + strand, within Pf1N1B4_4948 at 5485.671 kb on - strand, within Pf1N1B4_4948 at 5485.672 kb on + strand, within Pf1N1B4_4948 at 5485.673 kb on - strand, within Pf1N1B4_4948 at 5485.673 kb on - strand at 5485.673 kb on - strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand at 5485.709 kb on - strand, within Pf1N1B4_4948 at 5485.709 kb on - strand at 5485.709 kb on - strand at 5485.709 kb on - strand, within Pf1N1B4_4948 at 5485.858 kb on + strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5486.011 kb on - strand at 5486.011 kb on - strand at 5486.157 kb on + strand at 5486.157 kb on + strand, within Pf1N1B4_4949 at 5486.158 kb on - strand, within Pf1N1B4_4949 at 5486.158 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.307 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.672 kb on + strand at 5486.672 kb on + strand at 5486.783 kb on - strand, within Pf1N1B4_4950 at 5486.783 kb on - strand, within Pf1N1B4_4950 at 5487.247 kb on + strand, within Pf1N1B4_4950 at 5487.247 kb on + strand, within Pf1N1B4_4950 at 5487.319 kb on + strand, within Pf1N1B4_4950 at 5487.346 kb on + strand, within Pf1N1B4_4950 at 5487.347 kb on - strand, within Pf1N1B4_4950 at 5487.425 kb on - strand, within Pf1N1B4_4950 at 5487.580 kb on + strand at 5487.627 kb on + strand at 5487.712 kb on + strand at 5487.713 kb on - strand at 5487.713 kb on - strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.716 kb on + strand at 5487.717 kb on - strand at 5487.717 kb on - strand at 5487.717 kb on - strand at 5487.786 kb on + strand, within Pf1N1B4_4951 at 5487.787 kb on - strand at 5487.794 kb on + strand at 5487.813 kb on + strand, within Pf1N1B4_4951 at 5487.814 kb on - strand, within Pf1N1B4_4951 at 5487.814 kb on - strand, within Pf1N1B4_4951 at 5487.968 kb on - strand, within Pf1N1B4_4951 at 5487.970 kb on + strand, within Pf1N1B4_4951 at 5487.970 kb on + strand, within Pf1N1B4_4951 at 5488.073 kb on + strand at 5488.073 kb on + strand at 5488.074 kb on - strand at 5488.074 kb on - strand at 5488.076 kb on + strand at 5488.226 kb on - strand at 5488.363 kb on + strand at 5488.395 kb on + strand at 5488.395 kb on + strand at 5488.395 kb on + strand at 5488.396 kb on - strand at 5488.396 kb on - strand at 5488.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 5,485,670 + Pf1N1B4_4948 0.66 +0.7 5,485,671 - Pf1N1B4_4948 0.66 +0.4 5,485,672 + Pf1N1B4_4948 0.66 -0.7 5,485,673 - Pf1N1B4_4948 0.66 -2.2 5,485,673 - -1.3 5,485,673 - Pf1N1B4_4948 0.66 -1.2 5,485,708 + Pf1N1B4_4948 0.70 +0.6 5,485,708 + Pf1N1B4_4948 0.70 +2.4 5,485,708 + Pf1N1B4_4948 0.70 -0.3 5,485,708 + Pf1N1B4_4948 0.70 -0.6 5,485,708 + Pf1N1B4_4948 0.70 -0.2 5,485,708 + -0.7 5,485,709 - Pf1N1B4_4948 0.70 -0.4 5,485,709 - +1.5 5,485,709 - -1.1 5,485,709 - Pf1N1B4_4948 0.70 -0.8 5,485,858 + Pf1N1B4_4948 0.86 +0.3 5,485,859 - Pf1N1B4_4948 0.86 -0.5 5,485,859 - Pf1N1B4_4948 0.86 -3.0 5,485,859 - Pf1N1B4_4948 0.86 +0.3 5,486,011 - +0.7 5,486,011 - -0.0 5,486,157 + +1.5 5,486,157 + Pf1N1B4_4949 0.26 +0.7 5,486,158 - Pf1N1B4_4949 0.26 -0.0 5,486,158 - Pf1N1B4_4949 0.26 -1.4 5,486,221 - Pf1N1B4_4949 0.36 +0.4 5,486,221 - Pf1N1B4_4949 0.36 +0.6 5,486,221 - Pf1N1B4_4949 0.36 -0.7 5,486,307 + Pf1N1B4_4949 0.49 +0.6 5,486,493 + Pf1N1B4_4949 0.78 +0.8 5,486,493 + Pf1N1B4_4949 0.78 +0.9 5,486,493 + Pf1N1B4_4949 0.78 +0.2 5,486,672 + +0.6 5,486,672 + +0.0 5,486,783 - Pf1N1B4_4950 0.15 +0.6 5,486,783 - Pf1N1B4_4950 0.15 -0.3 5,487,247 + Pf1N1B4_4950 0.65 -2.6 5,487,247 + Pf1N1B4_4950 0.65 -1.1 5,487,319 + Pf1N1B4_4950 0.73 -0.8 5,487,346 + Pf1N1B4_4950 0.76 -0.9 5,487,347 - Pf1N1B4_4950 0.76 -0.7 5,487,425 - Pf1N1B4_4950 0.84 -0.8 5,487,580 + -0.5 5,487,627 + -0.0 5,487,712 + -0.8 5,487,713 - +0.4 5,487,713 - -0.8 5,487,716 + +0.1 5,487,716 + -0.5 5,487,716 + -1.3 5,487,716 + -0.1 5,487,716 + +0.0 5,487,716 + +0.3 5,487,717 - -0.5 5,487,717 - -1.0 5,487,717 - +1.1 5,487,786 + Pf1N1B4_4951 0.21 +0.2 5,487,787 - +1.9 5,487,794 + +0.3 5,487,813 + Pf1N1B4_4951 0.28 -0.6 5,487,814 - Pf1N1B4_4951 0.28 -0.7 5,487,814 - Pf1N1B4_4951 0.28 -0.7 5,487,968 - Pf1N1B4_4951 0.71 -0.1 5,487,970 + Pf1N1B4_4951 0.71 +0.4 5,487,970 + Pf1N1B4_4951 0.71 -0.0 5,488,073 + +0.3 5,488,073 + -1.3 5,488,074 - +0.2 5,488,074 - +0.6 5,488,076 + -1.1 5,488,226 - -0.6 5,488,363 + -0.7 5,488,395 + -0.6 5,488,395 + -1.4 5,488,395 + +0.2 5,488,396 - +0.5 5,488,396 - +0.9 5,488,412 - -1.0
Or see this region's nucleotide sequence