Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4828

Experiment: Minimal media pH7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4827 and Pf1N1B4_4828 overlap by 4 nucleotidesPf1N1B4_4828 and Pf1N1B4_4829 are separated by 3 nucleotides Pf1N1B4_4827 - Ethanolamine operon regulatory protein, at 5,332,010 to 5,332,936 _4827 Pf1N1B4_4828 - hypothetical protein, at 5,332,933 to 5,333,238 _4828 Pf1N1B4_4829 - Membrane protein, putative, at 5,333,242 to 5,334,234 _4829 Position (kb) 5332 5333 5334Strain fitness (log2 ratio) -1 0 1at 5331.958 kb on + strandat 5331.959 kb on - strandat 5331.959 kb on - strandat 5331.967 kb on + strandat 5332.170 kb on - strandat 5332.262 kb on + strandat 5332.523 kb on + strandat 5332.523 kb on + strand, within Pf1N1B4_4827at 5332.524 kb on - strand, within Pf1N1B4_4827at 5332.524 kb on - strand, within Pf1N1B4_4827at 5332.709 kb on + strand, within Pf1N1B4_4827at 5332.710 kb on - strandat 5332.982 kb on - strand, within Pf1N1B4_4828at 5332.984 kb on - strand, within Pf1N1B4_4828at 5333.092 kb on - strandat 5333.128 kb on - strand, within Pf1N1B4_4828at 5333.135 kb on + strand, within Pf1N1B4_4828at 5333.294 kb on + strandat 5333.360 kb on + strand, within Pf1N1B4_4829at 5333.360 kb on + strandat 5333.361 kb on - strandat 5334.000 kb on + strand, within Pf1N1B4_4829at 5334.000 kb on + strand, within Pf1N1B4_4829at 5334.001 kb on - strand, within Pf1N1B4_4829at 5334.001 kb on - strand, within Pf1N1B4_4829

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH7
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5,331,958 + -0.1
5,331,959 - +1.5
5,331,959 - +0.3
5,331,967 + +0.6
5,332,170 - -0.5
5,332,262 + +0.5
5,332,523 + -1.7
5,332,523 + Pf1N1B4_4827 0.55 -0.8
5,332,524 - Pf1N1B4_4827 0.55 +0.2
5,332,524 - Pf1N1B4_4827 0.55 -0.2
5,332,709 + Pf1N1B4_4827 0.75 -0.0
5,332,710 - -0.8
5,332,982 - Pf1N1B4_4828 0.16 -1.2
5,332,984 - Pf1N1B4_4828 0.17 -0.1
5,333,092 - -0.7
5,333,128 - Pf1N1B4_4828 0.64 -1.3
5,333,135 + Pf1N1B4_4828 0.66 -1.5
5,333,294 + +0.7
5,333,360 + Pf1N1B4_4829 0.12 +0.0
5,333,360 + -0.8
5,333,361 - +0.9
5,334,000 + Pf1N1B4_4829 0.76 +0.2
5,334,000 + Pf1N1B4_4829 0.76 +1.0
5,334,001 - Pf1N1B4_4829 0.76 -0.7
5,334,001 - Pf1N1B4_4829 0.76 -0.1

Or see this region's nucleotide sequence