Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4821 and Pf1N1B4_4822 overlap by 20 nucleotides Pf1N1B4_4822 and Pf1N1B4_4823 overlap by 4 nucleotides Pf1N1B4_4823 and Pf1N1B4_4824 are separated by 288 nucleotides
Pf1N1B4_4821 - Paraquat-inducible protein B, at 5,324,745 to 5,326,400
_4821
Pf1N1B4_4822 - Paraquat-inducible protein A, at 5,326,381 to 5,327,061
_4822
Pf1N1B4_4823 - Paraquat-inducible protein A, at 5,327,058 to 5,327,654
_4823
Pf1N1B4_4824 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 5,327,943 to 5,329,244
_4824
Position (kb)
5327
5328 Strain fitness (log2 ratio)
-2
-1
0
1 at 5326.272 kb on + strand at 5326.273 kb on - strand at 5326.498 kb on + strand, within Pf1N1B4_4822 at 5326.499 kb on - strand, within Pf1N1B4_4822 at 5326.499 kb on - strand, within Pf1N1B4_4822 at 5326.761 kb on + strand, within Pf1N1B4_4822 at 5326.883 kb on + strand, within Pf1N1B4_4822 at 5326.883 kb on + strand at 5326.884 kb on - strand, within Pf1N1B4_4822 at 5326.908 kb on - strand, within Pf1N1B4_4822 at 5327.061 kb on + strand at 5327.082 kb on + strand at 5327.083 kb on - strand at 5327.151 kb on + strand, within Pf1N1B4_4823 at 5327.154 kb on + strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.369 kb on + strand, within Pf1N1B4_4823 at 5327.461 kb on + strand, within Pf1N1B4_4823 at 5327.564 kb on + strand, within Pf1N1B4_4823 at 5327.564 kb on + strand, within Pf1N1B4_4823 at 5327.565 kb on - strand, within Pf1N1B4_4823 at 5327.605 kb on + strand at 5327.649 kb on - strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.703 kb on - strand at 5327.728 kb on + strand at 5327.729 kb on - strand at 5327.729 kb on - strand at 5327.756 kb on + strand at 5327.756 kb on + strand at 5327.757 kb on - strand at 5327.887 kb on + strand at 5327.888 kb on - strand at 5327.888 kb on - strand at 5328.229 kb on + strand at 5328.284 kb on + strand, within Pf1N1B4_4824 at 5328.285 kb on - strand, within Pf1N1B4_4824 at 5328.287 kb on - strand, within Pf1N1B4_4824 at 5328.319 kb on + strand, within Pf1N1B4_4824 at 5328.464 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 5,326,272 + -0.5 5,326,273 - -1.5 5,326,498 + Pf1N1B4_4822 0.17 -1.5 5,326,499 - Pf1N1B4_4822 0.17 +0.2 5,326,499 - Pf1N1B4_4822 0.17 -0.0 5,326,761 + Pf1N1B4_4822 0.56 -1.2 5,326,883 + Pf1N1B4_4822 0.74 -1.1 5,326,883 + +0.5 5,326,884 - Pf1N1B4_4822 0.74 +1.3 5,326,908 - Pf1N1B4_4822 0.77 +1.5 5,327,061 + -0.8 5,327,082 + -0.8 5,327,083 - +0.3 5,327,151 + Pf1N1B4_4823 0.16 -1.4 5,327,154 + Pf1N1B4_4823 0.16 -0.1 5,327,155 - Pf1N1B4_4823 0.16 -0.7 5,327,155 - Pf1N1B4_4823 0.16 -1.1 5,327,155 - Pf1N1B4_4823 0.16 -1.6 5,327,155 - Pf1N1B4_4823 0.16 -0.8 5,327,369 + Pf1N1B4_4823 0.52 +0.2 5,327,461 + Pf1N1B4_4823 0.68 -0.9 5,327,564 + Pf1N1B4_4823 0.85 -2.0 5,327,564 + Pf1N1B4_4823 0.85 -1.2 5,327,565 - Pf1N1B4_4823 0.85 +0.5 5,327,605 + -0.2 5,327,649 - -1.3 5,327,702 + +0.2 5,327,702 + +0.1 5,327,702 + +0.7 5,327,702 + -0.4 5,327,702 + -0.2 5,327,702 + -0.2 5,327,703 - -0.6 5,327,728 + +0.0 5,327,729 - +0.6 5,327,729 - -0.7 5,327,756 + +0.9 5,327,756 + +0.4 5,327,757 - -0.4 5,327,887 + -0.1 5,327,888 - -0.0 5,327,888 - +0.2 5,328,229 + +0.5 5,328,284 + Pf1N1B4_4824 0.26 -0.9 5,328,285 - Pf1N1B4_4824 0.26 -2.5 5,328,287 - Pf1N1B4_4824 0.26 -0.8 5,328,319 + Pf1N1B4_4824 0.29 -0.2 5,328,464 - +0.6
Or see this region's nucleotide sequence