Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4823

Experiment: Minimal media pH7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4821 and Pf1N1B4_4822 overlap by 20 nucleotidesPf1N1B4_4822 and Pf1N1B4_4823 overlap by 4 nucleotidesPf1N1B4_4823 and Pf1N1B4_4824 are separated by 288 nucleotides Pf1N1B4_4821 - Paraquat-inducible protein B, at 5,324,745 to 5,326,400 _4821 Pf1N1B4_4822 - Paraquat-inducible protein A, at 5,326,381 to 5,327,061 _4822 Pf1N1B4_4823 - Paraquat-inducible protein A, at 5,327,058 to 5,327,654 _4823 Pf1N1B4_4824 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 5,327,943 to 5,329,244 _4824 Position (kb) 5327 5328Strain fitness (log2 ratio) -2 -1 0 1at 5326.272 kb on + strandat 5326.273 kb on - strandat 5326.498 kb on + strand, within Pf1N1B4_4822at 5326.499 kb on - strand, within Pf1N1B4_4822at 5326.499 kb on - strand, within Pf1N1B4_4822at 5326.761 kb on + strand, within Pf1N1B4_4822at 5326.883 kb on + strand, within Pf1N1B4_4822at 5326.883 kb on + strandat 5326.884 kb on - strand, within Pf1N1B4_4822at 5326.908 kb on - strand, within Pf1N1B4_4822at 5327.061 kb on + strandat 5327.082 kb on + strandat 5327.083 kb on - strandat 5327.151 kb on + strand, within Pf1N1B4_4823at 5327.154 kb on + strand, within Pf1N1B4_4823at 5327.155 kb on - strand, within Pf1N1B4_4823at 5327.155 kb on - strand, within Pf1N1B4_4823at 5327.155 kb on - strand, within Pf1N1B4_4823at 5327.155 kb on - strand, within Pf1N1B4_4823at 5327.369 kb on + strand, within Pf1N1B4_4823at 5327.461 kb on + strand, within Pf1N1B4_4823at 5327.564 kb on + strand, within Pf1N1B4_4823at 5327.564 kb on + strand, within Pf1N1B4_4823at 5327.565 kb on - strand, within Pf1N1B4_4823at 5327.605 kb on + strandat 5327.649 kb on - strandat 5327.702 kb on + strandat 5327.702 kb on + strandat 5327.702 kb on + strandat 5327.702 kb on + strandat 5327.702 kb on + strandat 5327.702 kb on + strandat 5327.703 kb on - strandat 5327.728 kb on + strandat 5327.729 kb on - strandat 5327.729 kb on - strandat 5327.756 kb on + strandat 5327.756 kb on + strandat 5327.757 kb on - strandat 5327.887 kb on + strandat 5327.888 kb on - strandat 5327.888 kb on - strandat 5328.229 kb on + strandat 5328.284 kb on + strand, within Pf1N1B4_4824at 5328.285 kb on - strand, within Pf1N1B4_4824at 5328.287 kb on - strand, within Pf1N1B4_4824at 5328.319 kb on + strand, within Pf1N1B4_4824at 5328.464 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH7
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5,326,272 + -0.5
5,326,273 - -1.5
5,326,498 + Pf1N1B4_4822 0.17 -1.5
5,326,499 - Pf1N1B4_4822 0.17 +0.2
5,326,499 - Pf1N1B4_4822 0.17 -0.0
5,326,761 + Pf1N1B4_4822 0.56 -1.2
5,326,883 + Pf1N1B4_4822 0.74 -1.1
5,326,883 + +0.5
5,326,884 - Pf1N1B4_4822 0.74 +1.3
5,326,908 - Pf1N1B4_4822 0.77 +1.5
5,327,061 + -0.8
5,327,082 + -0.8
5,327,083 - +0.3
5,327,151 + Pf1N1B4_4823 0.16 -1.4
5,327,154 + Pf1N1B4_4823 0.16 -0.1
5,327,155 - Pf1N1B4_4823 0.16 -0.7
5,327,155 - Pf1N1B4_4823 0.16 -1.1
5,327,155 - Pf1N1B4_4823 0.16 -1.6
5,327,155 - Pf1N1B4_4823 0.16 -0.8
5,327,369 + Pf1N1B4_4823 0.52 +0.2
5,327,461 + Pf1N1B4_4823 0.68 -0.9
5,327,564 + Pf1N1B4_4823 0.85 -2.0
5,327,564 + Pf1N1B4_4823 0.85 -1.2
5,327,565 - Pf1N1B4_4823 0.85 +0.5
5,327,605 + -0.2
5,327,649 - -1.3
5,327,702 + +0.2
5,327,702 + +0.1
5,327,702 + +0.7
5,327,702 + -0.4
5,327,702 + -0.2
5,327,702 + -0.2
5,327,703 - -0.6
5,327,728 + +0.0
5,327,729 - +0.6
5,327,729 - -0.7
5,327,756 + +0.9
5,327,756 + +0.4
5,327,757 - -0.4
5,327,887 + -0.1
5,327,888 - -0.0
5,327,888 - +0.2
5,328,229 + +0.5
5,328,284 + Pf1N1B4_4824 0.26 -0.9
5,328,285 - Pf1N1B4_4824 0.26 -2.5
5,328,287 - Pf1N1B4_4824 0.26 -0.8
5,328,319 + Pf1N1B4_4824 0.29 -0.2
5,328,464 - +0.6

Or see this region's nucleotide sequence