Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4755

Experiment: Minimal media pH7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4754 and Pf1N1B4_4755 are separated by 201 nucleotidesPf1N1B4_4755 and Pf1N1B4_4756 are separated by 7 nucleotides Pf1N1B4_4754 - GNAT family acetyltransferase VC2332, at 5,251,694 to 5,252,791 _4754 Pf1N1B4_4755 - ribosomal protein S6 glutaminyl transferase related protein, at 5,252,993 to 5,254,471 _4755 Pf1N1B4_4756 - Magnesium and cobalt transport protein CorA, at 5,254,479 to 5,255,402 _4756 Position (kb) 5252 5253 5254 5255Strain fitness (log2 ratio) -3 -2 -1 0 1at 5252.028 kb on - strand, within Pf1N1B4_4754at 5252.028 kb on - strand, within Pf1N1B4_4754at 5252.040 kb on - strand, within Pf1N1B4_4754at 5252.102 kb on + strandat 5252.103 kb on - strand, within Pf1N1B4_4754at 5252.311 kb on - strand, within Pf1N1B4_4754at 5252.392 kb on - strand, within Pf1N1B4_4754at 5252.434 kb on - strandat 5252.578 kb on - strand, within Pf1N1B4_4754at 5252.792 kb on - strandat 5252.885 kb on - strandat 5253.010 kb on + strandat 5253.030 kb on - strandat 5253.132 kb on - strandat 5253.138 kb on - strandat 5253.138 kb on - strandat 5253.153 kb on - strand, within Pf1N1B4_4755at 5253.156 kb on - strand, within Pf1N1B4_4755at 5253.453 kb on + strand, within Pf1N1B4_4755at 5254.145 kb on + strand, within Pf1N1B4_4755at 5254.146 kb on - strand, within Pf1N1B4_4755at 5254.175 kb on + strand, within Pf1N1B4_4755at 5254.176 kb on - strand, within Pf1N1B4_4755at 5254.299 kb on - strand, within Pf1N1B4_4755at 5254.388 kb on + strandat 5254.388 kb on + strandat 5254.389 kb on - strandat 5254.389 kb on - strandat 5254.406 kb on + strandat 5254.406 kb on + strandat 5254.406 kb on + strandat 5254.704 kb on + strand, within Pf1N1B4_4756at 5254.704 kb on + strand, within Pf1N1B4_4756at 5254.871 kb on + strand, within Pf1N1B4_4756at 5254.881 kb on + strandat 5254.963 kb on + strand, within Pf1N1B4_4756at 5254.964 kb on - strand, within Pf1N1B4_4756at 5254.998 kb on + strand, within Pf1N1B4_4756at 5254.998 kb on + strand, within Pf1N1B4_4756at 5254.998 kb on + strand, within Pf1N1B4_4756at 5254.998 kb on + strandat 5254.998 kb on + strand, within Pf1N1B4_4756at 5254.998 kb on + strand, within Pf1N1B4_4756at 5254.999 kb on - strand, within Pf1N1B4_4756at 5254.999 kb on - strandat 5255.001 kb on + strand, within Pf1N1B4_4756at 5255.002 kb on - strand, within Pf1N1B4_4756at 5255.002 kb on - strand, within Pf1N1B4_4756at 5255.127 kb on + strand, within Pf1N1B4_4756at 5255.128 kb on - strand, within Pf1N1B4_4756at 5255.145 kb on + strand, within Pf1N1B4_4756at 5255.450 kb on + strandat 5255.450 kb on + strandat 5255.450 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH7
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5,252,028 - Pf1N1B4_4754 0.30 -0.9
5,252,028 - Pf1N1B4_4754 0.30 +0.6
5,252,040 - Pf1N1B4_4754 0.32 +1.4
5,252,102 + -1.6
5,252,103 - Pf1N1B4_4754 0.37 +0.0
5,252,311 - Pf1N1B4_4754 0.56 -2.4
5,252,392 - Pf1N1B4_4754 0.64 -0.2
5,252,434 - -0.1
5,252,578 - Pf1N1B4_4754 0.81 -0.1
5,252,792 - -1.4
5,252,885 - -0.3
5,253,010 + -0.6
5,253,030 - -0.2
5,253,132 - -0.2
5,253,138 - +1.2
5,253,138 - +0.4
5,253,153 - Pf1N1B4_4755 0.11 -0.1
5,253,156 - Pf1N1B4_4755 0.11 -0.4
5,253,453 + Pf1N1B4_4755 0.31 -0.3
5,254,145 + Pf1N1B4_4755 0.78 +0.3
5,254,146 - Pf1N1B4_4755 0.78 -0.6
5,254,175 + Pf1N1B4_4755 0.80 -1.6
5,254,176 - Pf1N1B4_4755 0.80 -1.9
5,254,299 - Pf1N1B4_4755 0.88 -2.8
5,254,388 + -0.9
5,254,388 + -0.1
5,254,389 - +0.3
5,254,389 - +0.6
5,254,406 + +0.0
5,254,406 + +0.7
5,254,406 + -0.7
5,254,704 + Pf1N1B4_4756 0.24 -0.3
5,254,704 + Pf1N1B4_4756 0.24 -0.3
5,254,871 + Pf1N1B4_4756 0.42 +0.7
5,254,881 + -0.9
5,254,963 + Pf1N1B4_4756 0.52 -0.1
5,254,964 - Pf1N1B4_4756 0.52 +0.1
5,254,998 + Pf1N1B4_4756 0.56 -1.1
5,254,998 + Pf1N1B4_4756 0.56 +0.7
5,254,998 + Pf1N1B4_4756 0.56 -1.0
5,254,998 + +1.2
5,254,998 + Pf1N1B4_4756 0.56 -0.7
5,254,998 + Pf1N1B4_4756 0.56 +0.5
5,254,999 - Pf1N1B4_4756 0.56 -0.1
5,254,999 - -0.4
5,255,001 + Pf1N1B4_4756 0.56 +0.1
5,255,002 - Pf1N1B4_4756 0.57 -0.3
5,255,002 - Pf1N1B4_4756 0.57 -0.2
5,255,127 + Pf1N1B4_4756 0.70 -0.3
5,255,128 - Pf1N1B4_4756 0.70 -0.6
5,255,145 + Pf1N1B4_4756 0.72 -1.4
5,255,450 + -1.8
5,255,450 + +0.0
5,255,450 + -0.9

Or see this region's nucleotide sequence