Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4754 and Pf1N1B4_4755 are separated by 201 nucleotides Pf1N1B4_4755 and Pf1N1B4_4756 are separated by 7 nucleotides
Pf1N1B4_4754 - GNAT family acetyltransferase VC2332, at 5,251,694 to 5,252,791
_4754
Pf1N1B4_4755 - ribosomal protein S6 glutaminyl transferase related protein, at 5,252,993 to 5,254,471
_4755
Pf1N1B4_4756 - Magnesium and cobalt transport protein CorA, at 5,254,479 to 5,255,402
_4756
Position (kb)
5252
5253
5254
5255 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.040 kb on - strand, within Pf1N1B4_4754 at 5252.102 kb on + strand at 5252.103 kb on - strand, within Pf1N1B4_4754 at 5252.311 kb on - strand, within Pf1N1B4_4754 at 5252.392 kb on - strand, within Pf1N1B4_4754 at 5252.434 kb on - strand at 5252.578 kb on - strand, within Pf1N1B4_4754 at 5252.792 kb on - strand at 5252.885 kb on - strand at 5253.010 kb on + strand at 5253.030 kb on - strand at 5253.132 kb on - strand at 5253.138 kb on - strand at 5253.138 kb on - strand at 5253.153 kb on - strand, within Pf1N1B4_4755 at 5253.156 kb on - strand, within Pf1N1B4_4755 at 5253.453 kb on + strand, within Pf1N1B4_4755 at 5254.145 kb on + strand, within Pf1N1B4_4755 at 5254.146 kb on - strand, within Pf1N1B4_4755 at 5254.175 kb on + strand, within Pf1N1B4_4755 at 5254.176 kb on - strand, within Pf1N1B4_4755 at 5254.299 kb on - strand, within Pf1N1B4_4755 at 5254.388 kb on + strand at 5254.388 kb on + strand at 5254.389 kb on - strand at 5254.389 kb on - strand at 5254.406 kb on + strand at 5254.406 kb on + strand at 5254.406 kb on + strand at 5254.704 kb on + strand, within Pf1N1B4_4756 at 5254.704 kb on + strand, within Pf1N1B4_4756 at 5254.871 kb on + strand, within Pf1N1B4_4756 at 5254.881 kb on + strand at 5254.963 kb on + strand, within Pf1N1B4_4756 at 5254.964 kb on - strand, within Pf1N1B4_4756 at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.998 kb on + strand at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.999 kb on - strand, within Pf1N1B4_4756 at 5254.999 kb on - strand at 5255.001 kb on + strand, within Pf1N1B4_4756 at 5255.002 kb on - strand, within Pf1N1B4_4756 at 5255.002 kb on - strand, within Pf1N1B4_4756 at 5255.127 kb on + strand, within Pf1N1B4_4756 at 5255.128 kb on - strand, within Pf1N1B4_4756 at 5255.145 kb on + strand, within Pf1N1B4_4756 at 5255.450 kb on + strand at 5255.450 kb on + strand at 5255.450 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 5,252,028 - Pf1N1B4_4754 0.30 -0.9 5,252,028 - Pf1N1B4_4754 0.30 +0.6 5,252,040 - Pf1N1B4_4754 0.32 +1.4 5,252,102 + -1.6 5,252,103 - Pf1N1B4_4754 0.37 +0.0 5,252,311 - Pf1N1B4_4754 0.56 -2.4 5,252,392 - Pf1N1B4_4754 0.64 -0.2 5,252,434 - -0.1 5,252,578 - Pf1N1B4_4754 0.81 -0.1 5,252,792 - -1.4 5,252,885 - -0.3 5,253,010 + -0.6 5,253,030 - -0.2 5,253,132 - -0.2 5,253,138 - +1.2 5,253,138 - +0.4 5,253,153 - Pf1N1B4_4755 0.11 -0.1 5,253,156 - Pf1N1B4_4755 0.11 -0.4 5,253,453 + Pf1N1B4_4755 0.31 -0.3 5,254,145 + Pf1N1B4_4755 0.78 +0.3 5,254,146 - Pf1N1B4_4755 0.78 -0.6 5,254,175 + Pf1N1B4_4755 0.80 -1.6 5,254,176 - Pf1N1B4_4755 0.80 -1.9 5,254,299 - Pf1N1B4_4755 0.88 -2.8 5,254,388 + -0.9 5,254,388 + -0.1 5,254,389 - +0.3 5,254,389 - +0.6 5,254,406 + +0.0 5,254,406 + +0.7 5,254,406 + -0.7 5,254,704 + Pf1N1B4_4756 0.24 -0.3 5,254,704 + Pf1N1B4_4756 0.24 -0.3 5,254,871 + Pf1N1B4_4756 0.42 +0.7 5,254,881 + -0.9 5,254,963 + Pf1N1B4_4756 0.52 -0.1 5,254,964 - Pf1N1B4_4756 0.52 +0.1 5,254,998 + Pf1N1B4_4756 0.56 -1.1 5,254,998 + Pf1N1B4_4756 0.56 +0.7 5,254,998 + Pf1N1B4_4756 0.56 -1.0 5,254,998 + +1.2 5,254,998 + Pf1N1B4_4756 0.56 -0.7 5,254,998 + Pf1N1B4_4756 0.56 +0.5 5,254,999 - Pf1N1B4_4756 0.56 -0.1 5,254,999 - -0.4 5,255,001 + Pf1N1B4_4756 0.56 +0.1 5,255,002 - Pf1N1B4_4756 0.57 -0.3 5,255,002 - Pf1N1B4_4756 0.57 -0.2 5,255,127 + Pf1N1B4_4756 0.70 -0.3 5,255,128 - Pf1N1B4_4756 0.70 -0.6 5,255,145 + Pf1N1B4_4756 0.72 -1.4 5,255,450 + -1.8 5,255,450 + +0.0 5,255,450 + -0.9
Or see this region's nucleotide sequence