Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_458

Experiment: Minimal media pH7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_457 and Pf1N1B4_458 overlap by 1 nucleotidesPf1N1B4_458 and Pf1N1B4_459 overlap by 14 nucleotides Pf1N1B4_457 - Periplasmic serine proteases (ClpP class), at 547,128 to 548,144 _457 Pf1N1B4_458 - Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C, at 548,144 to 548,806 _458 Pf1N1B4_459 - Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70), at 548,793 to 549,755 _459 Position (kb) 548 549Strain fitness (log2 ratio) -2 -1 0 1 2at 547.404 kb on - strand, within Pf1N1B4_457at 547.513 kb on + strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.518 kb on - strand, within Pf1N1B4_457at 547.612 kb on + strand, within Pf1N1B4_457at 547.613 kb on - strand, within Pf1N1B4_457at 547.639 kb on + strandat 547.639 kb on + strand, within Pf1N1B4_457at 547.639 kb on + strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.642 kb on + strand, within Pf1N1B4_457at 547.642 kb on + strandat 547.643 kb on - strand, within Pf1N1B4_457at 547.688 kb on + strandat 547.689 kb on - strand, within Pf1N1B4_457at 547.733 kb on - strand, within Pf1N1B4_457at 547.873 kb on - strand, within Pf1N1B4_457at 547.873 kb on - strand, within Pf1N1B4_457at 547.873 kb on - strand, within Pf1N1B4_457at 547.874 kb on + strand, within Pf1N1B4_457at 547.875 kb on - strand, within Pf1N1B4_457at 547.927 kb on - strand, within Pf1N1B4_457at 547.927 kb on - strand, within Pf1N1B4_457at 547.964 kb on - strand, within Pf1N1B4_457at 548.050 kb on - strandat 548.122 kb on + strandat 548.122 kb on + strandat 548.123 kb on - strandat 548.123 kb on - strandat 548.123 kb on - strandat 548.125 kb on - strandat 548.125 kb on - strandat 548.125 kb on - strandat 548.125 kb on - strandat 548.146 kb on - strandat 548.241 kb on + strandat 548.241 kb on + strand, within Pf1N1B4_458at 548.416 kb on - strand, within Pf1N1B4_458at 548.416 kb on - strand, within Pf1N1B4_458at 548.416 kb on - strand, within Pf1N1B4_458at 548.416 kb on - strand, within Pf1N1B4_458at 548.568 kb on + strand, within Pf1N1B4_458at 548.568 kb on + strand, within Pf1N1B4_458at 548.569 kb on - strand, within Pf1N1B4_458at 548.569 kb on - strand, within Pf1N1B4_458at 548.569 kb on - strand, within Pf1N1B4_458at 548.598 kb on - strand, within Pf1N1B4_458at 548.791 kb on - strandat 548.791 kb on - strandat 548.791 kb on - strandat 548.791 kb on - strandat 548.791 kb on - strandat 548.897 kb on - strand, within Pf1N1B4_459at 549.002 kb on + strand, within Pf1N1B4_459at 549.223 kb on + strand, within Pf1N1B4_459at 549.460 kb on + strand, within Pf1N1B4_459at 549.683 kb on + strandat 549.697 kb on + strandat 549.795 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH7
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547,404 - Pf1N1B4_457 0.27 +0.9
547,513 + Pf1N1B4_457 0.38 -0.1
547,514 - Pf1N1B4_457 0.38 +1.1
547,514 - Pf1N1B4_457 0.38 -0.4
547,514 - Pf1N1B4_457 0.38 -0.0
547,514 - Pf1N1B4_457 0.38 -0.7
547,514 - Pf1N1B4_457 0.38 +0.3
547,514 - Pf1N1B4_457 0.38 -2.6
547,518 - Pf1N1B4_457 0.38 -0.6
547,612 + Pf1N1B4_457 0.48 +0.5
547,613 - Pf1N1B4_457 0.48 -0.4
547,639 + -1.4
547,639 + Pf1N1B4_457 0.50 -0.4
547,639 + Pf1N1B4_457 0.50 +0.7
547,640 - Pf1N1B4_457 0.50 -0.4
547,640 - Pf1N1B4_457 0.50 -0.4
547,640 - Pf1N1B4_457 0.50 +1.5
547,640 - Pf1N1B4_457 0.50 -1.2
547,640 - Pf1N1B4_457 0.50 +1.0
547,640 - Pf1N1B4_457 0.50 -0.1
547,640 - Pf1N1B4_457 0.50 +0.1
547,640 - Pf1N1B4_457 0.50 -1.2
547,640 - Pf1N1B4_457 0.50 -0.2
547,640 - Pf1N1B4_457 0.50 +0.8
547,642 + Pf1N1B4_457 0.51 -0.9
547,642 + +1.9
547,643 - Pf1N1B4_457 0.51 -2.1
547,688 + -0.9
547,689 - Pf1N1B4_457 0.55 -0.0
547,733 - Pf1N1B4_457 0.59 +0.7
547,873 - Pf1N1B4_457 0.73 +0.6
547,873 - Pf1N1B4_457 0.73 -1.5
547,873 - Pf1N1B4_457 0.73 +0.8
547,874 + Pf1N1B4_457 0.73 -1.9
547,875 - Pf1N1B4_457 0.73 +0.8
547,927 - Pf1N1B4_457 0.79 -0.3
547,927 - Pf1N1B4_457 0.79 -0.6
547,964 - Pf1N1B4_457 0.82 -0.3
548,050 - +0.6
548,122 + -0.5
548,122 + -1.0
548,123 - +0.4
548,123 - +0.7
548,123 - -2.3
548,125 - +0.1
548,125 - -0.2
548,125 - +0.0
548,125 - +0.1
548,146 - -2.1
548,241 + -1.2
548,241 + Pf1N1B4_458 0.15 -0.2
548,416 - Pf1N1B4_458 0.41 -1.3
548,416 - Pf1N1B4_458 0.41 -0.9
548,416 - Pf1N1B4_458 0.41 -0.4
548,416 - Pf1N1B4_458 0.41 -0.9
548,568 + Pf1N1B4_458 0.64 -0.6
548,568 + Pf1N1B4_458 0.64 -1.6
548,569 - Pf1N1B4_458 0.64 -2.2
548,569 - Pf1N1B4_458 0.64 -0.5
548,569 - Pf1N1B4_458 0.64 -1.3
548,598 - Pf1N1B4_458 0.68 +0.0
548,791 - +0.4
548,791 - +0.7
548,791 - -2.1
548,791 - -0.6
548,791 - -0.3
548,897 - Pf1N1B4_459 0.11 -1.1
549,002 + Pf1N1B4_459 0.22 +0.2
549,223 + Pf1N1B4_459 0.45 +0.7
549,460 + Pf1N1B4_459 0.69 -0.9
549,683 + -0.4
549,697 + -0.7
549,795 + -0.1

Or see this region's nucleotide sequence