Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4201

Experiment: Minimal media pH7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4200 and Pf1N1B4_4201 overlap by 26 nucleotidesPf1N1B4_4201 and Pf1N1B4_4202 are separated by 155 nucleotidesPf1N1B4_4202 and Pf1N1B4_4203 overlap by 4 nucleotides Pf1N1B4_4200 - PhaK-like protein, at 4,651,788 to 4,653,035 _4200 Pf1N1B4_4201 - Transcriptional regulator, LysR family, at 4,653,010 to 4,654,245 _4201 Pf1N1B4_4202 - 4-oxalmesaconate hydratase (EC 4.2.1.83), at 4,654,401 to 4,655,135 _4202 Pf1N1B4_4203 - Putative siderophore biosynthesis protein, related to 2-demethylmenaquinone methyltransferase, at 4,655,132 to 4,655,848 _4203 Position (kb) 4653 4654 4655Strain fitness (log2 ratio) -2 -1 0 1 2at 4652.215 kb on + strand, within Pf1N1B4_4200at 4652.215 kb on + strand, within Pf1N1B4_4200at 4652.216 kb on - strandat 4652.298 kb on + strand, within Pf1N1B4_4200at 4652.484 kb on + strand, within Pf1N1B4_4200at 4652.485 kb on - strand, within Pf1N1B4_4200at 4652.485 kb on - strand, within Pf1N1B4_4200at 4652.636 kb on + strand, within Pf1N1B4_4200at 4652.643 kb on + strandat 4652.727 kb on + strand, within Pf1N1B4_4200at 4652.728 kb on - strand, within Pf1N1B4_4200at 4652.728 kb on - strandat 4652.740 kb on - strand, within Pf1N1B4_4200at 4652.829 kb on + strand, within Pf1N1B4_4200at 4653.166 kb on + strandat 4653.485 kb on + strand, within Pf1N1B4_4201at 4653.486 kb on - strand, within Pf1N1B4_4201at 4653.486 kb on - strand, within Pf1N1B4_4201at 4653.585 kb on + strand, within Pf1N1B4_4201at 4653.586 kb on - strandat 4654.281 kb on + strandat 4654.501 kb on - strand, within Pf1N1B4_4202at 4654.768 kb on - strand, within Pf1N1B4_4202at 4654.836 kb on + strand, within Pf1N1B4_4202at 4654.837 kb on - strand, within Pf1N1B4_4202at 4654.839 kb on - strand, within Pf1N1B4_4202at 4654.855 kb on + strandat 4654.959 kb on + strand, within Pf1N1B4_4202at 4655.004 kb on + strand, within Pf1N1B4_4202at 4655.004 kb on + strand, within Pf1N1B4_4202at 4655.007 kb on + strand, within Pf1N1B4_4202at 4655.007 kb on + strandat 4655.048 kb on + strand, within Pf1N1B4_4202at 4655.049 kb on - strand, within Pf1N1B4_4202at 4655.082 kb on + strandat 4655.082 kb on + strandat 4655.095 kb on - strandat 4655.173 kb on + strandat 4655.174 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH7
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4,652,215 + Pf1N1B4_4200 0.34 -0.7
4,652,215 + Pf1N1B4_4200 0.34 -1.1
4,652,216 - -0.9
4,652,298 + Pf1N1B4_4200 0.41 -2.3
4,652,484 + Pf1N1B4_4200 0.56 -0.2
4,652,485 - Pf1N1B4_4200 0.56 +0.3
4,652,485 - Pf1N1B4_4200 0.56 -0.7
4,652,636 + Pf1N1B4_4200 0.68 -0.3
4,652,643 + +1.2
4,652,727 + Pf1N1B4_4200 0.75 +0.5
4,652,728 - Pf1N1B4_4200 0.75 +0.8
4,652,728 - +1.9
4,652,740 - Pf1N1B4_4200 0.76 +0.3
4,652,829 + Pf1N1B4_4200 0.83 +0.6
4,653,166 + +1.6
4,653,485 + Pf1N1B4_4201 0.38 -0.5
4,653,486 - Pf1N1B4_4201 0.39 -1.4
4,653,486 - Pf1N1B4_4201 0.39 -0.5
4,653,585 + Pf1N1B4_4201 0.47 -0.8
4,653,586 - +0.6
4,654,281 + +0.1
4,654,501 - Pf1N1B4_4202 0.14 -0.4
4,654,768 - Pf1N1B4_4202 0.50 +1.1
4,654,836 + Pf1N1B4_4202 0.59 -1.4
4,654,837 - Pf1N1B4_4202 0.59 -1.2
4,654,839 - Pf1N1B4_4202 0.60 -0.5
4,654,855 + -1.2
4,654,959 + Pf1N1B4_4202 0.76 +0.0
4,655,004 + Pf1N1B4_4202 0.82 +0.7
4,655,004 + Pf1N1B4_4202 0.82 -0.6
4,655,007 + Pf1N1B4_4202 0.82 +0.2
4,655,007 + +1.0
4,655,048 + Pf1N1B4_4202 0.88 -0.3
4,655,049 - Pf1N1B4_4202 0.88 -0.1
4,655,082 + +0.5
4,655,082 + +0.0
4,655,095 - -1.5
4,655,173 + -1.0
4,655,174 - +0.9

Or see this region's nucleotide sequence