Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4200 and Pf1N1B4_4201 overlap by 26 nucleotides Pf1N1B4_4201 and Pf1N1B4_4202 are separated by 155 nucleotides Pf1N1B4_4202 and Pf1N1B4_4203 overlap by 4 nucleotides
Pf1N1B4_4200 - PhaK-like protein, at 4,651,788 to 4,653,035
_4200
Pf1N1B4_4201 - Transcriptional regulator, LysR family, at 4,653,010 to 4,654,245
_4201
Pf1N1B4_4202 - 4-oxalmesaconate hydratase (EC 4.2.1.83), at 4,654,401 to 4,655,135
_4202
Pf1N1B4_4203 - Putative siderophore biosynthesis protein, related to 2-demethylmenaquinone methyltransferase, at 4,655,132 to 4,655,848
_4203
Position (kb)
4653
4654
4655 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4652.215 kb on + strand, within Pf1N1B4_4200 at 4652.215 kb on + strand, within Pf1N1B4_4200 at 4652.216 kb on - strand at 4652.298 kb on + strand, within Pf1N1B4_4200 at 4652.484 kb on + strand, within Pf1N1B4_4200 at 4652.485 kb on - strand, within Pf1N1B4_4200 at 4652.485 kb on - strand, within Pf1N1B4_4200 at 4652.636 kb on + strand, within Pf1N1B4_4200 at 4652.643 kb on + strand at 4652.727 kb on + strand, within Pf1N1B4_4200 at 4652.728 kb on - strand, within Pf1N1B4_4200 at 4652.728 kb on - strand at 4652.740 kb on - strand, within Pf1N1B4_4200 at 4652.829 kb on + strand, within Pf1N1B4_4200 at 4653.166 kb on + strand at 4653.485 kb on + strand, within Pf1N1B4_4201 at 4653.486 kb on - strand, within Pf1N1B4_4201 at 4653.486 kb on - strand, within Pf1N1B4_4201 at 4653.585 kb on + strand, within Pf1N1B4_4201 at 4653.586 kb on - strand at 4654.281 kb on + strand at 4654.501 kb on - strand, within Pf1N1B4_4202 at 4654.768 kb on - strand, within Pf1N1B4_4202 at 4654.836 kb on + strand, within Pf1N1B4_4202 at 4654.837 kb on - strand, within Pf1N1B4_4202 at 4654.839 kb on - strand, within Pf1N1B4_4202 at 4654.855 kb on + strand at 4654.959 kb on + strand, within Pf1N1B4_4202 at 4655.004 kb on + strand, within Pf1N1B4_4202 at 4655.004 kb on + strand, within Pf1N1B4_4202 at 4655.007 kb on + strand, within Pf1N1B4_4202 at 4655.007 kb on + strand at 4655.048 kb on + strand, within Pf1N1B4_4202 at 4655.049 kb on - strand, within Pf1N1B4_4202 at 4655.082 kb on + strand at 4655.082 kb on + strand at 4655.095 kb on - strand at 4655.173 kb on + strand at 4655.174 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 4,652,215 + Pf1N1B4_4200 0.34 -0.7 4,652,215 + Pf1N1B4_4200 0.34 -1.1 4,652,216 - -0.9 4,652,298 + Pf1N1B4_4200 0.41 -2.3 4,652,484 + Pf1N1B4_4200 0.56 -0.2 4,652,485 - Pf1N1B4_4200 0.56 +0.3 4,652,485 - Pf1N1B4_4200 0.56 -0.7 4,652,636 + Pf1N1B4_4200 0.68 -0.3 4,652,643 + +1.2 4,652,727 + Pf1N1B4_4200 0.75 +0.5 4,652,728 - Pf1N1B4_4200 0.75 +0.8 4,652,728 - +1.9 4,652,740 - Pf1N1B4_4200 0.76 +0.3 4,652,829 + Pf1N1B4_4200 0.83 +0.6 4,653,166 + +1.6 4,653,485 + Pf1N1B4_4201 0.38 -0.5 4,653,486 - Pf1N1B4_4201 0.39 -1.4 4,653,486 - Pf1N1B4_4201 0.39 -0.5 4,653,585 + Pf1N1B4_4201 0.47 -0.8 4,653,586 - +0.6 4,654,281 + +0.1 4,654,501 - Pf1N1B4_4202 0.14 -0.4 4,654,768 - Pf1N1B4_4202 0.50 +1.1 4,654,836 + Pf1N1B4_4202 0.59 -1.4 4,654,837 - Pf1N1B4_4202 0.59 -1.2 4,654,839 - Pf1N1B4_4202 0.60 -0.5 4,654,855 + -1.2 4,654,959 + Pf1N1B4_4202 0.76 +0.0 4,655,004 + Pf1N1B4_4202 0.82 +0.7 4,655,004 + Pf1N1B4_4202 0.82 -0.6 4,655,007 + Pf1N1B4_4202 0.82 +0.2 4,655,007 + +1.0 4,655,048 + Pf1N1B4_4202 0.88 -0.3 4,655,049 - Pf1N1B4_4202 0.88 -0.1 4,655,082 + +0.5 4,655,082 + +0.0 4,655,095 - -1.5 4,655,173 + -1.0 4,655,174 - +0.9
Or see this region's nucleotide sequence