Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3725 and Pf1N1B4_3726 are separated by 32 nucleotides Pf1N1B4_3726 and Pf1N1B4_3727 are separated by 412 nucleotides
Pf1N1B4_3725 - Sensory box histidine kinase/response regulator, at 4,132,122 to 4,134,224
_3725
Pf1N1B4_3726 - Sensory box histidine kinase/response regulator, at 4,134,257 to 4,135,591
_3726
Pf1N1B4_3727 - C4-dicarboxylate transporter/malic acid transport protein, at 4,136,004 to 4,137,155
_3727
Position (kb)
4134
4135
4136 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4133.441 kb on + strand, within Pf1N1B4_3725 at 4133.581 kb on + strand, within Pf1N1B4_3725 at 4133.806 kb on - strand, within Pf1N1B4_3725 at 4133.888 kb on - strand, within Pf1N1B4_3725 at 4133.967 kb on + strand, within Pf1N1B4_3725 at 4133.967 kb on + strand, within Pf1N1B4_3725 at 4133.967 kb on + strand, within Pf1N1B4_3725 at 4133.967 kb on + strand, within Pf1N1B4_3725 at 4133.968 kb on - strand, within Pf1N1B4_3725 at 4134.232 kb on + strand at 4134.232 kb on + strand at 4134.232 kb on + strand at 4134.233 kb on - strand at 4134.233 kb on - strand at 4134.233 kb on - strand at 4134.233 kb on - strand at 4134.298 kb on - strand at 4134.491 kb on - strand, within Pf1N1B4_3726 at 4134.566 kb on - strand, within Pf1N1B4_3726 at 4134.732 kb on + strand, within Pf1N1B4_3726 at 4134.794 kb on + strand, within Pf1N1B4_3726 at 4134.794 kb on + strand, within Pf1N1B4_3726 at 4134.794 kb on + strand, within Pf1N1B4_3726 at 4134.795 kb on - strand, within Pf1N1B4_3726 at 4134.943 kb on - strand, within Pf1N1B4_3726 at 4134.943 kb on - strand, within Pf1N1B4_3726 at 4135.200 kb on + strand, within Pf1N1B4_3726 at 4135.200 kb on + strand, within Pf1N1B4_3726 at 4135.200 kb on + strand, within Pf1N1B4_3726 at 4135.201 kb on - strand, within Pf1N1B4_3726 at 4135.374 kb on + strand, within Pf1N1B4_3726 at 4135.374 kb on + strand, within Pf1N1B4_3726 at 4135.468 kb on - strand at 4135.468 kb on - strand at 4135.468 kb on - strand at 4135.519 kb on - strand at 4135.546 kb on + strand at 4135.547 kb on - strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.548 kb on + strand at 4135.549 kb on - strand at 4135.549 kb on - strand at 4135.549 kb on - strand at 4135.549 kb on - strand at 4135.549 kb on - strand at 4135.549 kb on - strand at 4135.549 kb on - strand at 4135.549 kb on - strand at 4135.659 kb on + strand at 4135.659 kb on + strand at 4135.660 kb on - strand at 4135.660 kb on - strand at 4135.660 kb on - strand at 4135.789 kb on + strand at 4135.789 kb on + strand at 4135.790 kb on - strand at 4135.844 kb on - strand at 4135.860 kb on - strand at 4135.965 kb on + strand at 4136.122 kb on + strand, within Pf1N1B4_3727 at 4136.122 kb on + strand at 4136.122 kb on + strand, within Pf1N1B4_3727 at 4136.122 kb on + strand, within Pf1N1B4_3727 at 4136.122 kb on + strand, within Pf1N1B4_3727 at 4136.123 kb on - strand, within Pf1N1B4_3727 at 4136.123 kb on - strand, within Pf1N1B4_3727 at 4136.216 kb on + strand, within Pf1N1B4_3727 at 4136.216 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.232 kb on + strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.233 kb on - strand, within Pf1N1B4_3727 at 4136.443 kb on + strand, within Pf1N1B4_3727 at 4136.454 kb on + strand, within Pf1N1B4_3727 at 4136.455 kb on - strand, within Pf1N1B4_3727 at 4136.455 kb on - strand, within Pf1N1B4_3727
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 4,133,441 + Pf1N1B4_3725 0.63 -1.2 4,133,581 + Pf1N1B4_3725 0.69 -0.1 4,133,806 - Pf1N1B4_3725 0.80 -2.1 4,133,888 - Pf1N1B4_3725 0.84 -0.7 4,133,967 + Pf1N1B4_3725 0.88 -0.2 4,133,967 + Pf1N1B4_3725 0.88 +2.0 4,133,967 + Pf1N1B4_3725 0.88 -0.2 4,133,967 + Pf1N1B4_3725 0.88 -0.6 4,133,968 - Pf1N1B4_3725 0.88 -0.5 4,134,232 + -1.3 4,134,232 + -0.5 4,134,232 + +0.5 4,134,233 - +1.3 4,134,233 - -0.3 4,134,233 - +0.1 4,134,233 - -2.1 4,134,298 - -1.2 4,134,491 - Pf1N1B4_3726 0.18 -0.1 4,134,566 - Pf1N1B4_3726 0.23 -0.9 4,134,732 + Pf1N1B4_3726 0.36 +0.0 4,134,794 + Pf1N1B4_3726 0.40 -0.6 4,134,794 + Pf1N1B4_3726 0.40 -0.4 4,134,794 + Pf1N1B4_3726 0.40 -2.3 4,134,795 - Pf1N1B4_3726 0.40 -2.2 4,134,943 - Pf1N1B4_3726 0.51 -0.2 4,134,943 - Pf1N1B4_3726 0.51 +0.3 4,135,200 + Pf1N1B4_3726 0.71 -0.2 4,135,200 + Pf1N1B4_3726 0.71 +0.8 4,135,200 + Pf1N1B4_3726 0.71 +1.8 4,135,201 - Pf1N1B4_3726 0.71 +1.7 4,135,374 + Pf1N1B4_3726 0.84 -0.4 4,135,374 + Pf1N1B4_3726 0.84 -0.2 4,135,468 - +0.6 4,135,468 - -0.9 4,135,468 - +0.8 4,135,519 - +0.0 4,135,546 + -0.3 4,135,547 - +0.0 4,135,548 + +0.0 4,135,548 + +0.8 4,135,548 + +0.4 4,135,548 + +0.3 4,135,548 + -0.7 4,135,548 + -0.7 4,135,548 + +1.2 4,135,548 + +0.7 4,135,548 + -0.2 4,135,548 + -0.1 4,135,549 - -0.5 4,135,549 - -1.9 4,135,549 - -0.2 4,135,549 - -0.6 4,135,549 - -0.7 4,135,549 - +0.5 4,135,549 - -1.1 4,135,549 - -0.9 4,135,659 + -1.0 4,135,659 + +0.3 4,135,660 - -1.7 4,135,660 - -0.3 4,135,660 - -0.4 4,135,789 + +0.8 4,135,789 + +0.0 4,135,790 - -0.3 4,135,844 - -1.3 4,135,860 - +1.5 4,135,965 + +0.5 4,136,122 + Pf1N1B4_3727 0.10 +0.0 4,136,122 + +1.3 4,136,122 + Pf1N1B4_3727 0.10 +1.0 4,136,122 + Pf1N1B4_3727 0.10 -0.7 4,136,122 + Pf1N1B4_3727 0.10 +0.2 4,136,123 - Pf1N1B4_3727 0.10 +1.0 4,136,123 - Pf1N1B4_3727 0.10 +1.2 4,136,216 + Pf1N1B4_3727 0.18 -0.6 4,136,216 + Pf1N1B4_3727 0.18 -2.0 4,136,232 + Pf1N1B4_3727 0.20 -0.3 4,136,232 + Pf1N1B4_3727 0.20 -1.0 4,136,232 + Pf1N1B4_3727 0.20 -0.7 4,136,232 + Pf1N1B4_3727 0.20 -0.9 4,136,232 + Pf1N1B4_3727 0.20 +0.2 4,136,232 + Pf1N1B4_3727 0.20 +0.0 4,136,232 + Pf1N1B4_3727 0.20 +1.1 4,136,232 + -1.0 4,136,232 + Pf1N1B4_3727 0.20 +1.0 4,136,232 + Pf1N1B4_3727 0.20 +0.4 4,136,233 - Pf1N1B4_3727 0.20 -0.0 4,136,233 - Pf1N1B4_3727 0.20 +0.6 4,136,233 - Pf1N1B4_3727 0.20 -0.7 4,136,233 - Pf1N1B4_3727 0.20 -0.3 4,136,233 - Pf1N1B4_3727 0.20 -0.5 4,136,233 - Pf1N1B4_3727 0.20 +0.8 4,136,233 - Pf1N1B4_3727 0.20 -0.1 4,136,233 - Pf1N1B4_3727 0.20 -2.2 4,136,233 - Pf1N1B4_3727 0.20 -0.2 4,136,443 + Pf1N1B4_3727 0.38 +1.1 4,136,454 + Pf1N1B4_3727 0.39 -1.4 4,136,455 - Pf1N1B4_3727 0.39 -1.9 4,136,455 - Pf1N1B4_3727 0.39 -0.3
Or see this region's nucleotide sequence