Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3713 and Pf1N1B4_3714 overlap by 4 nucleotides Pf1N1B4_3714 and Pf1N1B4_3715 are separated by 2 nucleotides Pf1N1B4_3715 and Pf1N1B4_3716 are separated by 170 nucleotides Pf1N1B4_3716 and Pf1N1B4_3717 are separated by 52 nucleotides
Pf1N1B4_3713 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), at 4,121,750 to 4,122,805
_3713
Pf1N1B4_3714 - Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73), at 4,122,802 to 4,123,377
_3714
Pf1N1B4_3715 - Cobalamin synthase, at 4,123,380 to 4,124,111
_3715
Pf1N1B4_3716 - Transcriptional regulator, MarR family, at 4,124,282 to 4,124,668
_3716
Pf1N1B4_3717 - Permeases of the major facilitator superfamily, at 4,124,721 to 4,125,929
_3717
Position (kb) 4123
4124
4125 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4122.433 kb on + strand, within Pf1N1B4_3713 at 4122.544 kb on + strand, within Pf1N1B4_3713 at 4122.545 kb on - strand at 4123.126 kb on + strand, within Pf1N1B4_3714 at 4123.126 kb on + strand, within Pf1N1B4_3714 at 4123.211 kb on - strand, within Pf1N1B4_3714 at 4123.736 kb on - strand at 4123.736 kb on - strand at 4123.848 kb on + strand, within Pf1N1B4_3715 at 4123.848 kb on + strand, within Pf1N1B4_3715 at 4123.848 kb on + strand, within Pf1N1B4_3715 at 4123.848 kb on + strand, within Pf1N1B4_3715 at 4123.848 kb on + strand, within Pf1N1B4_3715 at 4123.848 kb on + strand at 4123.849 kb on - strand, within Pf1N1B4_3715 at 4123.849 kb on - strand, within Pf1N1B4_3715 at 4123.849 kb on - strand, within Pf1N1B4_3715 at 4123.849 kb on - strand, within Pf1N1B4_3715 at 4123.967 kb on - strand, within Pf1N1B4_3715 at 4124.049 kb on - strand at 4124.214 kb on + strand at 4124.214 kb on + strand at 4124.214 kb on + strand at 4124.214 kb on + strand at 4124.214 kb on + strand at 4124.214 kb on + strand at 4124.214 kb on + strand at 4124.214 kb on + strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.215 kb on - strand at 4124.219 kb on - strand at 4124.219 kb on - strand at 4124.219 kb on - strand at 4124.251 kb on - strand at 4124.251 kb on - strand at 4124.252 kb on + strand at 4124.254 kb on + strand at 4124.254 kb on + strand at 4124.254 kb on + strand at 4124.254 kb on + strand at 4124.255 kb on - strand at 4124.255 kb on - strand at 4124.255 kb on - strand at 4124.263 kb on + strand at 4124.290 kb on + strand at 4124.328 kb on - strand, within Pf1N1B4_3716 at 4124.697 kb on + strand at 4124.812 kb on + strand at 4124.813 kb on - strand at 4124.973 kb on + strand, within Pf1N1B4_3717
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 4,122,433 + Pf1N1B4_3713 0.65 +1.0 4,122,544 + Pf1N1B4_3713 0.75 -2.0 4,122,545 - -1.3 4,123,126 + Pf1N1B4_3714 0.56 -1.7 4,123,126 + Pf1N1B4_3714 0.56 -1.3 4,123,211 - Pf1N1B4_3714 0.71 -0.5 4,123,736 - +0.4 4,123,736 - -1.1 4,123,848 + Pf1N1B4_3715 0.64 -1.3 4,123,848 + Pf1N1B4_3715 0.64 -1.5 4,123,848 + Pf1N1B4_3715 0.64 -0.9 4,123,848 + Pf1N1B4_3715 0.64 -2.9 4,123,848 + Pf1N1B4_3715 0.64 -0.6 4,123,848 + -0.1 4,123,849 - Pf1N1B4_3715 0.64 -0.1 4,123,849 - Pf1N1B4_3715 0.64 +0.6 4,123,849 - Pf1N1B4_3715 0.64 -0.8 4,123,849 - Pf1N1B4_3715 0.64 -0.6 4,123,967 - Pf1N1B4_3715 0.80 -1.7 4,124,049 - +1.0 4,124,214 + +0.3 4,124,214 + -0.3 4,124,214 + -0.1 4,124,214 + -0.3 4,124,214 + +0.2 4,124,214 + +0.5 4,124,214 + +1.5 4,124,214 + -0.3 4,124,215 - -2.3 4,124,215 - -1.3 4,124,215 - -1.0 4,124,215 - -0.1 4,124,215 - +1.2 4,124,215 - -1.5 4,124,215 - +0.0 4,124,215 - +0.3 4,124,215 - +0.2 4,124,215 - -2.1 4,124,215 - +0.2 4,124,219 - +0.4 4,124,219 - +0.1 4,124,219 - +0.9 4,124,251 - +0.9 4,124,251 - -0.7 4,124,252 + -0.8 4,124,254 + -0.5 4,124,254 + -1.1 4,124,254 + +1.5 4,124,254 + +0.1 4,124,255 - -1.5 4,124,255 - -2.1 4,124,255 - -1.1 4,124,263 + -1.1 4,124,290 + -0.5 4,124,328 - Pf1N1B4_3716 0.12 -1.0 4,124,697 + -0.2 4,124,812 + +0.1 4,124,813 - -0.0 4,124,973 + Pf1N1B4_3717 0.21 -0.0
Or see this region's nucleotide sequence