Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3707

Experiment: Minimal media pH7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3706 and Pf1N1B4_3707 are separated by 10 nucleotidesPf1N1B4_3707 and Pf1N1B4_3708 overlap by 4 nucleotidesPf1N1B4_3708 and Pf1N1B4_3709 overlap by 4 nucleotides Pf1N1B4_3706 - Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 4,115,042 to 4,115,653 _3706 Pf1N1B4_3707 - Cobyrinic acid A,C-diamide synthase, at 4,115,664 to 4,117,046 _3707 Pf1N1B4_3708 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 4,117,043 to 4,117,693 _3708 Pf1N1B4_3709 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 4,117,690 to 4,118,598 _3709 Position (kb) 4115 4116 4117 4118Strain fitness (log2 ratio) -2 -1 0 1at 4114.698 kb on + strandat 4114.846 kb on + strandat 4114.847 kb on - strandat 4114.993 kb on + strandat 4114.994 kb on - strandat 4114.994 kb on - strandat 4115.664 kb on - strandat 4115.664 kb on - strandat 4115.664 kb on - strandat 4115.850 kb on + strand, within Pf1N1B4_3707at 4115.896 kb on + strand, within Pf1N1B4_3707at 4115.897 kb on - strand, within Pf1N1B4_3707at 4116.417 kb on - strand, within Pf1N1B4_3707at 4116.420 kb on + strand, within Pf1N1B4_3707at 4116.517 kb on - strandat 4116.517 kb on - strand, within Pf1N1B4_3707at 4116.550 kb on + strandat 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.551 kb on - strand, within Pf1N1B4_3707at 4116.551 kb on - strand, within Pf1N1B4_3707at 4116.642 kb on + strand, within Pf1N1B4_3707at 4116.642 kb on + strand, within Pf1N1B4_3707at 4116.642 kb on + strand, within Pf1N1B4_3707at 4116.643 kb on - strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strandat 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.900 kb on + strand, within Pf1N1B4_3707at 4116.900 kb on + strand, within Pf1N1B4_3707at 4116.900 kb on + strandat 4116.901 kb on - strand, within Pf1N1B4_3707at 4116.901 kb on - strand, within Pf1N1B4_3707at 4116.912 kb on + strandat 4116.912 kb on + strandat 4117.149 kb on + strand, within Pf1N1B4_3708at 4117.149 kb on + strand, within Pf1N1B4_3708at 4117.359 kb on + strand, within Pf1N1B4_3708at 4117.360 kb on - strand, within Pf1N1B4_3708at 4117.620 kb on + strand, within Pf1N1B4_3708at 4117.664 kb on + strandat 4117.664 kb on + strandat 4117.665 kb on - strandat 4117.673 kb on + strandat 4117.674 kb on - strandat 4117.909 kb on + strand, within Pf1N1B4_3709at 4117.909 kb on + strand, within Pf1N1B4_3709at 4117.942 kb on + strandat 4117.942 kb on + strandat 4117.942 kb on + strand, within Pf1N1B4_3709

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH7
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4,114,698 + +0.3
4,114,846 + +0.4
4,114,847 - +0.3
4,114,993 + +0.1
4,114,994 - -0.8
4,114,994 - -0.7
4,115,664 - -0.6
4,115,664 - +1.1
4,115,664 - +0.3
4,115,850 + Pf1N1B4_3707 0.13 -0.9
4,115,896 + Pf1N1B4_3707 0.17 -0.7
4,115,897 - Pf1N1B4_3707 0.17 +0.7
4,116,417 - Pf1N1B4_3707 0.54 -0.3
4,116,420 + Pf1N1B4_3707 0.55 -0.2
4,116,517 - -0.5
4,116,517 - Pf1N1B4_3707 0.62 -0.1
4,116,550 + +0.3
4,116,550 + Pf1N1B4_3707 0.64 -0.1
4,116,550 + Pf1N1B4_3707 0.64 -0.2
4,116,550 + Pf1N1B4_3707 0.64 -1.0
4,116,550 + Pf1N1B4_3707 0.64 -1.9
4,116,550 + Pf1N1B4_3707 0.64 -1.3
4,116,551 - Pf1N1B4_3707 0.64 -1.6
4,116,551 - Pf1N1B4_3707 0.64 -2.0
4,116,642 + Pf1N1B4_3707 0.71 -0.4
4,116,642 + Pf1N1B4_3707 0.71 +1.2
4,116,642 + Pf1N1B4_3707 0.71 -0.8
4,116,643 - Pf1N1B4_3707 0.71 -0.3
4,116,789 + Pf1N1B4_3707 0.81 -0.1
4,116,789 + Pf1N1B4_3707 0.81 -0.5
4,116,789 + +0.4
4,116,789 + Pf1N1B4_3707 0.81 -0.0
4,116,789 + Pf1N1B4_3707 0.81 -1.3
4,116,789 + Pf1N1B4_3707 0.81 -0.3
4,116,789 + Pf1N1B4_3707 0.81 -2.6
4,116,900 + Pf1N1B4_3707 0.89 -0.6
4,116,900 + Pf1N1B4_3707 0.89 -0.9
4,116,900 + +0.5
4,116,901 - Pf1N1B4_3707 0.89 -2.7
4,116,901 - Pf1N1B4_3707 0.89 -0.4
4,116,912 + -0.2
4,116,912 + +0.3
4,117,149 + Pf1N1B4_3708 0.16 -0.4
4,117,149 + Pf1N1B4_3708 0.16 -0.7
4,117,359 + Pf1N1B4_3708 0.49 -2.1
4,117,360 - Pf1N1B4_3708 0.49 +0.1
4,117,620 + Pf1N1B4_3708 0.89 +0.6
4,117,664 + +0.1
4,117,664 + -1.3
4,117,665 - -0.2
4,117,673 + +0.7
4,117,674 - -0.3
4,117,909 + Pf1N1B4_3709 0.24 -0.6
4,117,909 + Pf1N1B4_3709 0.24 +0.2
4,117,942 + -0.5
4,117,942 + -0.7
4,117,942 + Pf1N1B4_3709 0.28 -1.0

Or see this region's nucleotide sequence