Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3394 and Pf1N1B4_3395 are separated by 270 nucleotides Pf1N1B4_3395 and Pf1N1B4_3396 are separated by 18 nucleotides Pf1N1B4_3396 and Pf1N1B4_3397 are separated by 141 nucleotides Pf1N1B4_3397 and Pf1N1B4_3398 are separated by 100 nucleotides
Pf1N1B4_3394 - Aquaporin Z, at 3,787,964 to 3,788,662
_3394
Pf1N1B4_3395 - Phosphohydrolase (MutT/nudix family protein), at 3,788,933 to 3,789,538
_3395
Pf1N1B4_3396 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,789,557 to 3,790,042
_3396
Pf1N1B4_3397 - Ketosteroid isomerase homolog, at 3,790,184 to 3,790,609
_3397
Pf1N1B4_3398 - DGPFAETKE, at 3,790,710 to 3,791,054
_3398
Position (kb)
3789
3790
3791 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3788.662 kb on + strand at 3788.663 kb on - strand at 3788.663 kb on - strand at 3788.663 kb on - strand at 3788.683 kb on + strand at 3788.683 kb on + strand at 3788.683 kb on + strand at 3788.684 kb on - strand at 3788.715 kb on + strand at 3788.715 kb on + strand at 3788.715 kb on + strand at 3788.716 kb on - strand at 3788.736 kb on + strand at 3788.769 kb on + strand at 3788.769 kb on + strand at 3788.769 kb on + strand at 3788.793 kb on - strand at 3788.793 kb on - strand at 3788.820 kb on + strand at 3788.821 kb on - strand at 3788.821 kb on - strand at 3788.821 kb on - strand at 3788.938 kb on - strand at 3788.945 kb on - strand at 3788.976 kb on + strand at 3788.976 kb on + strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3789.447 kb on + strand, within Pf1N1B4_3395 at 3789.504 kb on + strand at 3789.504 kb on + strand at 3789.505 kb on - strand at 3789.603 kb on + strand at 3789.603 kb on + strand at 3789.603 kb on + strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.630 kb on + strand, within Pf1N1B4_3396 at 3789.631 kb on - strand, within Pf1N1B4_3396 at 3789.639 kb on + strand, within Pf1N1B4_3396 at 3789.639 kb on + strand, within Pf1N1B4_3396 at 3789.640 kb on - strand, within Pf1N1B4_3396 at 3790.021 kb on - strand at 3790.110 kb on + strand at 3790.146 kb on + strand at 3790.223 kb on + strand at 3790.224 kb on - strand at 3790.334 kb on + strand, within Pf1N1B4_3397 at 3790.660 kb on + strand at 3790.660 kb on + strand at 3790.660 kb on + strand at 3790.661 kb on - strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.720 kb on + strand at 3790.720 kb on + strand at 3790.720 kb on + strand at 3790.720 kb on + strand at 3790.720 kb on + strand at 3790.721 kb on - strand at 3790.721 kb on - strand at 3790.721 kb on - strand at 3790.731 kb on + strand at 3790.731 kb on + strand at 3790.731 kb on + strand at 3790.731 kb on + strand at 3790.732 kb on - strand at 3790.732 kb on - strand at 3790.734 kb on - strand at 3790.734 kb on - strand at 3790.734 kb on - strand at 3790.944 kb on + strand, within Pf1N1B4_3398
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 3,788,662 + -0.3 3,788,663 - -1.1 3,788,663 - -0.3 3,788,663 - +0.1 3,788,683 + +1.1 3,788,683 + +0.9 3,788,683 + +0.5 3,788,684 - -0.7 3,788,715 + +1.4 3,788,715 + +0.7 3,788,715 + -0.7 3,788,716 - -0.7 3,788,736 + +1.2 3,788,769 + +1.1 3,788,769 + -0.6 3,788,769 + -0.3 3,788,793 - +0.6 3,788,793 - +2.0 3,788,820 + +1.3 3,788,821 - +1.8 3,788,821 - -1.3 3,788,821 - -0.6 3,788,938 - +0.4 3,788,945 - +1.7 3,788,976 + +0.3 3,788,976 + -1.4 3,788,977 - +0.4 3,788,977 - -0.3 3,788,977 - +1.3 3,788,977 - +0.1 3,788,977 - -0.1 3,788,977 - -0.2 3,788,977 - +0.9 3,788,977 - -1.4 3,789,447 + Pf1N1B4_3395 0.85 -0.1 3,789,504 + -1.1 3,789,504 + -0.5 3,789,505 - +0.4 3,789,603 + -0.5 3,789,603 + -0.5 3,789,603 + +0.5 3,789,604 - +0.1 3,789,604 - -0.1 3,789,604 - -0.8 3,789,604 - +0.4 3,789,630 + Pf1N1B4_3396 0.15 -0.7 3,789,631 - Pf1N1B4_3396 0.15 -0.8 3,789,639 + Pf1N1B4_3396 0.17 -1.3 3,789,639 + Pf1N1B4_3396 0.17 -1.7 3,789,640 - Pf1N1B4_3396 0.17 -0.0 3,790,021 - -0.1 3,790,110 + +1.2 3,790,146 + +1.0 3,790,223 + -0.3 3,790,224 - -1.2 3,790,334 + Pf1N1B4_3397 0.35 -1.4 3,790,660 + -0.6 3,790,660 + -1.0 3,790,660 + -0.0 3,790,661 - +0.1 3,790,716 + -0.2 3,790,716 + +1.0 3,790,716 + +0.7 3,790,716 + -1.6 3,790,716 + -0.1 3,790,716 + +1.4 3,790,716 + +0.0 3,790,716 + +0.0 3,790,717 - +0.4 3,790,717 - +1.0 3,790,717 - +0.4 3,790,717 - +1.0 3,790,717 - +0.3 3,790,717 - -1.0 3,790,717 - -0.1 3,790,717 - -0.2 3,790,717 - -2.8 3,790,720 + -1.6 3,790,720 + +1.1 3,790,720 + -1.4 3,790,720 + +1.0 3,790,720 + -0.8 3,790,721 - +0.8 3,790,721 - +0.2 3,790,721 - +0.1 3,790,731 + -1.9 3,790,731 + -0.8 3,790,731 + -0.6 3,790,731 + -0.0 3,790,732 - -1.0 3,790,732 - -0.2 3,790,734 - -0.4 3,790,734 - -0.5 3,790,734 - -0.1 3,790,944 + Pf1N1B4_3398 0.68 -0.2
Or see this region's nucleotide sequence