Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_336 and Pf1N1B4_337 overlap by 11 nucleotides Pf1N1B4_337 and Pf1N1B4_338 are separated by 117 nucleotides Pf1N1B4_338 and Pf1N1B4_339 are separated by 359 nucleotides
Pf1N1B4_336 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 399,494 to 400,861
_336
Pf1N1B4_337 - O-antigen export system permease protein RfbD, at 400,851 to 401,669
_337
Pf1N1B4_338 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 401,787 to 402,794
_338
Pf1N1B4_339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 403,154 to 404,116
_339
Position (kb) 401
402
403 Strain fitness (log2 ratio) -2
-1
0
1
2 at 400.806 kb on + strand at 400.807 kb on - strand at 400.807 kb on - strand at 400.810 kb on + strand at 400.810 kb on + strand at 400.811 kb on - strand at 400.849 kb on + strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.850 kb on - strand at 400.852 kb on + strand at 400.874 kb on + strand at 400.928 kb on + strand at 400.929 kb on - strand at 400.930 kb on + strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.931 kb on - strand at 400.933 kb on + strand, within Pf1N1B4_337 at 400.933 kb on + strand, within Pf1N1B4_337 at 400.934 kb on - strand, within Pf1N1B4_337 at 400.934 kb on - strand at 400.934 kb on - strand, within Pf1N1B4_337 at 400.934 kb on - strand, within Pf1N1B4_337 at 400.960 kb on - strand, within Pf1N1B4_337 at 400.960 kb on - strand, within Pf1N1B4_337 at 401.216 kb on + strand, within Pf1N1B4_337 at 401.217 kb on - strand, within Pf1N1B4_337 at 401.217 kb on - strand, within Pf1N1B4_337 at 401.264 kb on + strand, within Pf1N1B4_337 at 401.316 kb on + strand, within Pf1N1B4_337 at 401.344 kb on - strand, within Pf1N1B4_337 at 401.386 kb on - strand, within Pf1N1B4_337 at 401.405 kb on + strand, within Pf1N1B4_337 at 401.406 kb on - strand, within Pf1N1B4_337 at 401.406 kb on - strand, within Pf1N1B4_337 at 401.432 kb on + strand, within Pf1N1B4_337 at 401.432 kb on + strand, within Pf1N1B4_337 at 401.655 kb on - strand at 401.675 kb on - strand at 401.735 kb on + strand at 402.084 kb on - strand, within Pf1N1B4_338 at 402.086 kb on - strand, within Pf1N1B4_338 at 402.086 kb on - strand, within Pf1N1B4_338 at 402.145 kb on + strand, within Pf1N1B4_338 at 402.198 kb on + strand, within Pf1N1B4_338 at 402.443 kb on - strand, within Pf1N1B4_338 at 402.474 kb on - strand at 402.554 kb on - strand, within Pf1N1B4_338 at 402.881 kb on - strand at 402.930 kb on - strand at 403.004 kb on - strand at 403.016 kb on + strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.148 kb on + strand at 403.148 kb on + strand at 403.149 kb on - strand at 403.159 kb on + strand at 403.493 kb on + strand, within Pf1N1B4_339 at 403.765 kb on + strand, within Pf1N1B4_339
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 400,806 + +0.0 400,807 - +0.8 400,807 - -1.2 400,810 + +0.9 400,810 + -2.0 400,811 - +0.4 400,849 + -1.0 400,850 - +0.2 400,850 - +0.4 400,850 - +0.3 400,850 - +0.1 400,850 - +0.1 400,852 + +0.9 400,874 + -0.5 400,928 + +0.7 400,929 - -0.4 400,930 + -1.6 400,931 - +0.1 400,931 - +1.0 400,931 - -0.1 400,931 - -2.3 400,931 - +0.2 400,931 - -0.7 400,931 - -0.4 400,931 - -0.4 400,931 - -0.2 400,933 + Pf1N1B4_337 0.10 +0.4 400,933 + Pf1N1B4_337 0.10 +0.1 400,934 - Pf1N1B4_337 0.10 -0.2 400,934 - -0.3 400,934 - Pf1N1B4_337 0.10 +0.5 400,934 - Pf1N1B4_337 0.10 -0.2 400,960 - Pf1N1B4_337 0.13 -1.1 400,960 - Pf1N1B4_337 0.13 +0.0 401,216 + Pf1N1B4_337 0.45 +0.9 401,217 - Pf1N1B4_337 0.45 +0.3 401,217 - Pf1N1B4_337 0.45 -1.4 401,264 + Pf1N1B4_337 0.50 +1.0 401,316 + Pf1N1B4_337 0.57 -1.8 401,344 - Pf1N1B4_337 0.60 -0.4 401,386 - Pf1N1B4_337 0.65 +0.7 401,405 + Pf1N1B4_337 0.68 +0.7 401,406 - Pf1N1B4_337 0.68 -1.2 401,406 - Pf1N1B4_337 0.68 +0.0 401,432 + Pf1N1B4_337 0.71 -0.7 401,432 + Pf1N1B4_337 0.71 -0.7 401,655 - -0.1 401,675 - +0.4 401,735 + -0.3 402,084 - Pf1N1B4_338 0.29 -1.3 402,086 - Pf1N1B4_338 0.30 -1.4 402,086 - Pf1N1B4_338 0.30 -2.2 402,145 + Pf1N1B4_338 0.36 +0.4 402,198 + Pf1N1B4_338 0.41 +1.4 402,443 - Pf1N1B4_338 0.65 -1.4 402,474 - +0.9 402,554 - Pf1N1B4_338 0.76 -1.7 402,881 - -1.3 402,930 - -0.3 403,004 - -0.8 403,016 + -1.7 403,017 - +0.2 403,017 - -0.6 403,017 - +1.9 403,017 - +0.1 403,148 + +0.1 403,148 + -0.1 403,149 - +0.3 403,159 + -0.6 403,493 + Pf1N1B4_339 0.35 -0.3 403,765 + Pf1N1B4_339 0.63 -1.0
Or see this region's nucleotide sequence