Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3097 and Pf1N1B4_3098 overlap by 1 nucleotides Pf1N1B4_3098 and Pf1N1B4_3099 are separated by 82 nucleotides
Pf1N1B4_3097 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 3,480,609 to 3,481,736
_3097
Pf1N1B4_3098 - Ribonuclease HII (EC 3.1.26.4), at 3,481,736 to 3,482,377
_3098
Pf1N1B4_3099 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,482,460 to 3,485,981
_3099
Position (kb)
3481
3482
3483 Strain fitness (log2 ratio)
-2
-1
0
1 at 3481.734 kb on + strand at 3481.734 kb on + strand at 3481.735 kb on - strand at 3481.735 kb on - strand at 3481.771 kb on + strand at 3481.791 kb on + strand at 3481.791 kb on + strand at 3481.791 kb on + strand at 3481.792 kb on - strand at 3481.792 kb on - strand at 3481.792 kb on - strand at 3481.970 kb on + strand, within Pf1N1B4_3098 at 3481.970 kb on + strand, within Pf1N1B4_3098 at 3481.970 kb on + strand, within Pf1N1B4_3098 at 3481.970 kb on + strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.990 kb on - strand, within Pf1N1B4_3098 at 3482.063 kb on - strand, within Pf1N1B4_3098 at 3482.345 kb on + strand at 3482.375 kb on + strand at 3482.394 kb on + strand at 3482.394 kb on + strand at 3482.394 kb on + strand at 3482.439 kb on + strand at 3482.446 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 3,481,734 + -0.5 3,481,734 + +0.6 3,481,735 - -1.1 3,481,735 - +0.3 3,481,771 + +0.1 3,481,791 + -0.3 3,481,791 + -1.2 3,481,791 + +0.5 3,481,792 - -0.1 3,481,792 - -0.6 3,481,792 - +0.2 3,481,970 + Pf1N1B4_3098 0.36 -1.4 3,481,970 + Pf1N1B4_3098 0.36 -0.5 3,481,970 + Pf1N1B4_3098 0.36 -0.5 3,481,970 + Pf1N1B4_3098 0.36 -1.4 3,481,971 - Pf1N1B4_3098 0.37 -0.8 3,481,971 - Pf1N1B4_3098 0.37 +0.5 3,481,971 - Pf1N1B4_3098 0.37 -2.6 3,481,971 - Pf1N1B4_3098 0.37 +0.0 3,481,971 - Pf1N1B4_3098 0.37 -0.1 3,481,990 - Pf1N1B4_3098 0.40 -1.9 3,482,063 - Pf1N1B4_3098 0.51 -0.1 3,482,345 + +0.2 3,482,375 + +0.8 3,482,394 + -0.1 3,482,394 + -0.9 3,482,394 + +0.8 3,482,439 + +0.5 3,482,446 + -1.2
Or see this region's nucleotide sequence