Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3098

Experiment: Minimal media pH7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3097 and Pf1N1B4_3098 overlap by 1 nucleotidesPf1N1B4_3098 and Pf1N1B4_3099 are separated by 82 nucleotides Pf1N1B4_3097 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 3,480,609 to 3,481,736 _3097 Pf1N1B4_3098 - Ribonuclease HII (EC 3.1.26.4), at 3,481,736 to 3,482,377 _3098 Pf1N1B4_3099 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,482,460 to 3,485,981 _3099 Position (kb) 3481 3482 3483Strain fitness (log2 ratio) -2 -1 0 1at 3481.734 kb on + strandat 3481.734 kb on + strandat 3481.735 kb on - strandat 3481.735 kb on - strandat 3481.771 kb on + strandat 3481.791 kb on + strandat 3481.791 kb on + strandat 3481.791 kb on + strandat 3481.792 kb on - strandat 3481.792 kb on - strandat 3481.792 kb on - strandat 3481.970 kb on + strand, within Pf1N1B4_3098at 3481.970 kb on + strand, within Pf1N1B4_3098at 3481.970 kb on + strand, within Pf1N1B4_3098at 3481.970 kb on + strand, within Pf1N1B4_3098at 3481.971 kb on - strand, within Pf1N1B4_3098at 3481.971 kb on - strand, within Pf1N1B4_3098at 3481.971 kb on - strand, within Pf1N1B4_3098at 3481.971 kb on - strand, within Pf1N1B4_3098at 3481.971 kb on - strand, within Pf1N1B4_3098at 3481.990 kb on - strand, within Pf1N1B4_3098at 3482.063 kb on - strand, within Pf1N1B4_3098at 3482.345 kb on + strandat 3482.375 kb on + strandat 3482.394 kb on + strandat 3482.394 kb on + strandat 3482.394 kb on + strandat 3482.439 kb on + strandat 3482.446 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH7
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3,481,734 + -0.5
3,481,734 + +0.6
3,481,735 - -1.1
3,481,735 - +0.3
3,481,771 + +0.1
3,481,791 + -0.3
3,481,791 + -1.2
3,481,791 + +0.5
3,481,792 - -0.1
3,481,792 - -0.6
3,481,792 - +0.2
3,481,970 + Pf1N1B4_3098 0.36 -1.4
3,481,970 + Pf1N1B4_3098 0.36 -0.5
3,481,970 + Pf1N1B4_3098 0.36 -0.5
3,481,970 + Pf1N1B4_3098 0.36 -1.4
3,481,971 - Pf1N1B4_3098 0.37 -0.8
3,481,971 - Pf1N1B4_3098 0.37 +0.5
3,481,971 - Pf1N1B4_3098 0.37 -2.6
3,481,971 - Pf1N1B4_3098 0.37 +0.0
3,481,971 - Pf1N1B4_3098 0.37 -0.1
3,481,990 - Pf1N1B4_3098 0.40 -1.9
3,482,063 - Pf1N1B4_3098 0.51 -0.1
3,482,345 + +0.2
3,482,375 + +0.8
3,482,394 + -0.1
3,482,394 + -0.9
3,482,394 + +0.8
3,482,439 + +0.5
3,482,446 + -1.2

Or see this region's nucleotide sequence