Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2957 and Pf1N1B4_2958 overlap by 10 nucleotides Pf1N1B4_2958 and Pf1N1B4_2959 are separated by 334 nucleotides Pf1N1B4_2959 and Pf1N1B4_2960 are separated by 28 nucleotides
Pf1N1B4_2957 - Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-), at 3,310,529 to 3,311,830
_2957
Pf1N1B4_2958 - Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-), at 3,311,821 to 3,312,210
_2958
Pf1N1B4_2959 - FIG067310: hypothetical protein, at 3,312,545 to 3,314,434
_2959
Pf1N1B4_2960 - FIG002188: hypothetical protein, at 3,314,463 to 3,315,827
_2960
Position (kb)
3312
3313
3314
3315 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3311.609 kb on + strand, within Pf1N1B4_2957 at 3311.741 kb on + strand at 3311.742 kb on - strand at 3311.742 kb on - strand at 3311.742 kb on - strand at 3311.936 kb on + strand, within Pf1N1B4_2958 at 3311.936 kb on + strand, within Pf1N1B4_2958 at 3311.936 kb on + strand, within Pf1N1B4_2958 at 3311.937 kb on - strand at 3311.937 kb on - strand, within Pf1N1B4_2958 at 3312.105 kb on + strand, within Pf1N1B4_2958 at 3312.189 kb on + strand at 3312.189 kb on + strand at 3312.189 kb on + strand at 3312.189 kb on + strand at 3312.189 kb on + strand at 3312.189 kb on + strand at 3312.189 kb on + strand at 3312.189 kb on + strand at 3312.190 kb on - strand at 3312.190 kb on - strand at 3312.190 kb on - strand at 3312.190 kb on - strand at 3312.209 kb on - strand at 3312.383 kb on + strand at 3312.384 kb on - strand at 3312.501 kb on + strand at 3312.507 kb on + strand at 3312.507 kb on + strand at 3312.523 kb on + strand at 3312.523 kb on + strand at 3312.524 kb on - strand at 3312.524 kb on - strand at 3312.524 kb on - strand at 3312.700 kb on + strand at 3313.002 kb on - strand, within Pf1N1B4_2959 at 3313.066 kb on + strand, within Pf1N1B4_2959 at 3313.067 kb on - strand, within Pf1N1B4_2959 at 3313.172 kb on + strand, within Pf1N1B4_2959 at 3313.172 kb on + strand, within Pf1N1B4_2959 at 3313.172 kb on + strand, within Pf1N1B4_2959 at 3313.172 kb on + strand, within Pf1N1B4_2959 at 3313.172 kb on + strand, within Pf1N1B4_2959 at 3313.173 kb on - strand, within Pf1N1B4_2959 at 3313.218 kb on - strand, within Pf1N1B4_2959 at 3313.218 kb on - strand, within Pf1N1B4_2959 at 3313.218 kb on - strand, within Pf1N1B4_2959 at 3313.282 kb on + strand, within Pf1N1B4_2959 at 3313.461 kb on - strand, within Pf1N1B4_2959 at 3313.461 kb on - strand, within Pf1N1B4_2959 at 3313.509 kb on - strand, within Pf1N1B4_2959 at 3313.883 kb on - strand, within Pf1N1B4_2959 at 3314.199 kb on + strand, within Pf1N1B4_2959 at 3314.378 kb on + strand at 3314.432 kb on + strand at 3314.432 kb on + strand at 3314.433 kb on - strand at 3314.433 kb on - strand at 3314.433 kb on - strand at 3314.433 kb on - strand at 3314.433 kb on - strand at 3314.441 kb on + strand at 3314.441 kb on + strand at 3314.441 kb on + strand at 3314.442 kb on - strand at 3314.442 kb on - strand at 3314.462 kb on + strand at 3314.462 kb on + strand at 3314.463 kb on - strand at 3314.463 kb on - strand at 3314.470 kb on + strand at 3314.739 kb on + strand, within Pf1N1B4_2960 at 3314.740 kb on - strand, within Pf1N1B4_2960 at 3314.812 kb on - strand, within Pf1N1B4_2960 at 3314.882 kb on + strand, within Pf1N1B4_2960 at 3315.057 kb on + strand, within Pf1N1B4_2960 at 3315.057 kb on + strand, within Pf1N1B4_2960 at 3315.057 kb on + strand, within Pf1N1B4_2960 at 3315.099 kb on + strand, within Pf1N1B4_2960 at 3315.100 kb on - strand at 3315.132 kb on + strand, within Pf1N1B4_2960 at 3315.132 kb on + strand, within Pf1N1B4_2960 at 3315.133 kb on - strand, within Pf1N1B4_2960 at 3315.133 kb on - strand, within Pf1N1B4_2960 at 3315.133 kb on - strand, within Pf1N1B4_2960 at 3315.234 kb on + strand, within Pf1N1B4_2960 at 3315.235 kb on - strand, within Pf1N1B4_2960 at 3315.235 kb on - strand, within Pf1N1B4_2960 at 3315.280 kb on - strand, within Pf1N1B4_2960 at 3315.280 kb on - strand, within Pf1N1B4_2960 at 3315.280 kb on - strand, within Pf1N1B4_2960 at 3315.280 kb on - strand, within Pf1N1B4_2960 at 3315.280 kb on - strand, within Pf1N1B4_2960 at 3315.280 kb on - strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.336 kb on + strand, within Pf1N1B4_2960 at 3315.337 kb on - strand, within Pf1N1B4_2960 at 3315.337 kb on - strand at 3315.337 kb on - strand, within Pf1N1B4_2960 at 3315.337 kb on - strand, within Pf1N1B4_2960 at 3315.337 kb on - strand, within Pf1N1B4_2960 at 3315.337 kb on - strand, within Pf1N1B4_2960 at 3315.337 kb on - strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.390 kb on + strand, within Pf1N1B4_2960 at 3315.391 kb on - strand, within Pf1N1B4_2960 at 3315.391 kb on - strand, within Pf1N1B4_2960 at 3315.391 kb on - strand, within Pf1N1B4_2960 at 3315.391 kb on - strand, within Pf1N1B4_2960 at 3315.391 kb on - strand, within Pf1N1B4_2960 at 3315.391 kb on - strand at 3315.391 kb on - strand, within Pf1N1B4_2960 at 3315.391 kb on - strand, within Pf1N1B4_2960 at 3315.408 kb on + strand, within Pf1N1B4_2960 at 3315.408 kb on + strand, within Pf1N1B4_2960 at 3315.408 kb on + strand, within Pf1N1B4_2960 at 3315.408 kb on + strand, within Pf1N1B4_2960 at 3315.408 kb on + strand, within Pf1N1B4_2960 at 3315.408 kb on + strand, within Pf1N1B4_2960 at 3315.408 kb on + strand, within Pf1N1B4_2960 at 3315.408 kb on + strand, within Pf1N1B4_2960 at 3315.409 kb on - strand, within Pf1N1B4_2960 at 3315.409 kb on - strand, within Pf1N1B4_2960 at 3315.409 kb on - strand, within Pf1N1B4_2960
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 3,311,609 + Pf1N1B4_2957 0.83 -0.7 3,311,741 + +0.3 3,311,742 - +1.0 3,311,742 - -0.2 3,311,742 - -0.6 3,311,936 + Pf1N1B4_2958 0.29 -0.7 3,311,936 + Pf1N1B4_2958 0.29 -2.0 3,311,936 + Pf1N1B4_2958 0.29 -0.1 3,311,937 - +2.1 3,311,937 - Pf1N1B4_2958 0.30 +1.2 3,312,105 + Pf1N1B4_2958 0.73 -0.2 3,312,189 + +0.3 3,312,189 + +1.2 3,312,189 + +0.4 3,312,189 + +0.1 3,312,189 + -0.1 3,312,189 + +0.0 3,312,189 + -0.4 3,312,189 + -0.3 3,312,190 - +0.5 3,312,190 - +0.1 3,312,190 - +0.8 3,312,190 - +0.6 3,312,209 - +1.5 3,312,383 + -0.8 3,312,384 - -0.7 3,312,501 + -0.2 3,312,507 + +0.3 3,312,507 + +0.7 3,312,523 + -1.6 3,312,523 + +0.2 3,312,524 - -0.1 3,312,524 - +1.1 3,312,524 - +0.4 3,312,700 + +0.5 3,313,002 - Pf1N1B4_2959 0.24 -0.9 3,313,066 + Pf1N1B4_2959 0.28 +0.1 3,313,067 - Pf1N1B4_2959 0.28 -0.0 3,313,172 + Pf1N1B4_2959 0.33 -1.4 3,313,172 + Pf1N1B4_2959 0.33 -2.2 3,313,172 + Pf1N1B4_2959 0.33 -1.0 3,313,172 + Pf1N1B4_2959 0.33 +0.9 3,313,172 + Pf1N1B4_2959 0.33 -0.7 3,313,173 - Pf1N1B4_2959 0.33 +0.5 3,313,218 - Pf1N1B4_2959 0.36 +0.9 3,313,218 - Pf1N1B4_2959 0.36 -1.1 3,313,218 - Pf1N1B4_2959 0.36 -1.2 3,313,282 + Pf1N1B4_2959 0.39 -1.0 3,313,461 - Pf1N1B4_2959 0.48 +0.2 3,313,461 - Pf1N1B4_2959 0.48 +1.1 3,313,509 - Pf1N1B4_2959 0.51 -1.1 3,313,883 - Pf1N1B4_2959 0.71 -0.6 3,314,199 + Pf1N1B4_2959 0.88 +0.2 3,314,378 + -2.8 3,314,432 + +1.4 3,314,432 + -0.1 3,314,433 - +0.1 3,314,433 - -0.3 3,314,433 - +0.1 3,314,433 - -0.8 3,314,433 - -0.7 3,314,441 + -0.2 3,314,441 + -0.4 3,314,441 + -0.1 3,314,442 - -1.9 3,314,442 - +1.5 3,314,462 + +1.2 3,314,462 + +0.2 3,314,463 - -1.1 3,314,463 - +0.3 3,314,470 + -1.2 3,314,739 + Pf1N1B4_2960 0.20 -0.9 3,314,740 - Pf1N1B4_2960 0.20 -1.7 3,314,812 - Pf1N1B4_2960 0.26 +1.0 3,314,882 + Pf1N1B4_2960 0.31 -0.8 3,315,057 + Pf1N1B4_2960 0.44 +0.3 3,315,057 + Pf1N1B4_2960 0.44 -0.9 3,315,057 + Pf1N1B4_2960 0.44 +1.5 3,315,099 + Pf1N1B4_2960 0.47 +1.3 3,315,100 - -1.1 3,315,132 + Pf1N1B4_2960 0.49 -0.8 3,315,132 + Pf1N1B4_2960 0.49 +0.2 3,315,133 - Pf1N1B4_2960 0.49 -1.0 3,315,133 - Pf1N1B4_2960 0.49 +0.4 3,315,133 - Pf1N1B4_2960 0.49 -1.7 3,315,234 + Pf1N1B4_2960 0.56 -0.5 3,315,235 - Pf1N1B4_2960 0.57 +0.7 3,315,235 - Pf1N1B4_2960 0.57 +1.4 3,315,280 - Pf1N1B4_2960 0.60 +0.3 3,315,280 - Pf1N1B4_2960 0.60 +0.8 3,315,280 - Pf1N1B4_2960 0.60 +0.6 3,315,280 - Pf1N1B4_2960 0.60 -0.8 3,315,280 - Pf1N1B4_2960 0.60 +1.1 3,315,280 - Pf1N1B4_2960 0.60 +0.6 3,315,336 + Pf1N1B4_2960 0.64 +0.1 3,315,336 + Pf1N1B4_2960 0.64 -0.9 3,315,336 + Pf1N1B4_2960 0.64 +0.2 3,315,336 + +0.5 3,315,336 + Pf1N1B4_2960 0.64 -1.0 3,315,336 + Pf1N1B4_2960 0.64 -0.8 3,315,336 + Pf1N1B4_2960 0.64 -0.7 3,315,336 + Pf1N1B4_2960 0.64 +1.0 3,315,336 + Pf1N1B4_2960 0.64 +0.2 3,315,336 + +1.5 3,315,336 + Pf1N1B4_2960 0.64 -2.1 3,315,336 + Pf1N1B4_2960 0.64 +0.1 3,315,336 + Pf1N1B4_2960 0.64 +0.5 3,315,337 - Pf1N1B4_2960 0.64 -1.5 3,315,337 - +0.1 3,315,337 - Pf1N1B4_2960 0.64 +0.0 3,315,337 - Pf1N1B4_2960 0.64 +0.3 3,315,337 - Pf1N1B4_2960 0.64 -0.7 3,315,337 - Pf1N1B4_2960 0.64 +1.2 3,315,337 - Pf1N1B4_2960 0.64 -0.9 3,315,390 + Pf1N1B4_2960 0.68 -0.2 3,315,390 + Pf1N1B4_2960 0.68 -2.0 3,315,390 + Pf1N1B4_2960 0.68 +0.7 3,315,390 + Pf1N1B4_2960 0.68 -1.4 3,315,390 + Pf1N1B4_2960 0.68 +0.3 3,315,390 + Pf1N1B4_2960 0.68 -0.0 3,315,390 + Pf1N1B4_2960 0.68 -1.1 3,315,390 + Pf1N1B4_2960 0.68 +0.2 3,315,390 + Pf1N1B4_2960 0.68 -0.2 3,315,390 + Pf1N1B4_2960 0.68 +0.8 3,315,390 + Pf1N1B4_2960 0.68 +0.2 3,315,390 + Pf1N1B4_2960 0.68 +0.4 3,315,391 - Pf1N1B4_2960 0.68 -0.1 3,315,391 - Pf1N1B4_2960 0.68 +0.7 3,315,391 - Pf1N1B4_2960 0.68 -0.4 3,315,391 - Pf1N1B4_2960 0.68 -1.0 3,315,391 - Pf1N1B4_2960 0.68 -1.6 3,315,391 - -1.1 3,315,391 - Pf1N1B4_2960 0.68 +0.9 3,315,391 - Pf1N1B4_2960 0.68 +0.2 3,315,408 + Pf1N1B4_2960 0.69 +1.0 3,315,408 + Pf1N1B4_2960 0.69 -1.5 3,315,408 + Pf1N1B4_2960 0.69 +0.3 3,315,408 + Pf1N1B4_2960 0.69 -0.1 3,315,408 + Pf1N1B4_2960 0.69 +0.8 3,315,408 + Pf1N1B4_2960 0.69 +0.4 3,315,408 + Pf1N1B4_2960 0.69 -0.0 3,315,408 + Pf1N1B4_2960 0.69 +0.2 3,315,409 - Pf1N1B4_2960 0.69 +0.3 3,315,409 - Pf1N1B4_2960 0.69 -0.4 3,315,409 - Pf1N1B4_2960 0.69 +0.8
Or see this region's nucleotide sequence