Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2922 and Pf1N1B4_2923 are separated by 120 nucleotides Pf1N1B4_2923 and Pf1N1B4_2924 are separated by 136 nucleotides Pf1N1B4_2924 and Pf1N1B4_2925 overlap by 86 nucleotides Pf1N1B4_2925 and Pf1N1B4_2926 are separated by 23 nucleotides Pf1N1B4_2926 and Pf1N1B4_2927 are separated by 86 nucleotides
Pf1N1B4_2922 - Glutamate racemase (EC 5.1.1.3), at 3,280,206 to 3,280,997
_2922
Pf1N1B4_2923 - Lipid A 3-O-deacylase, at 3,281,118 to 3,281,636
_2923
Pf1N1B4_2924 - FIG00955589: hypothetical protein, at 3,281,773 to 3,282,078
_2924
Pf1N1B4_2925 - hypothetical protein, at 3,281,993 to 3,282,115
_2925
Pf1N1B4_2926 - Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-), at 3,282,139 to 3,282,885
_2926
Pf1N1B4_2927 - Deoxyribodipyrimidine photolyase (EC 4.1.99.3), at 3,282,972 to 3,284,453
_2927
Position (kb)
3281
3282
3283 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3280.995 kb on + strand at 3280.996 kb on - strand at 3280.999 kb on + strand at 3281.020 kb on + strand at 3281.055 kb on + strand at 3281.055 kb on + strand at 3281.055 kb on + strand at 3281.056 kb on - strand at 3281.056 kb on - strand at 3281.057 kb on + strand at 3281.057 kb on + strand at 3281.058 kb on - strand at 3281.079 kb on + strand at 3281.079 kb on + strand at 3281.088 kb on + strand at 3281.088 kb on + strand at 3281.089 kb on - strand at 3281.117 kb on - strand at 3281.128 kb on + strand at 3281.235 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.320 kb on - strand, within Pf1N1B4_2923 at 3281.325 kb on + strand, within Pf1N1B4_2923 at 3281.325 kb on + strand, within Pf1N1B4_2923 at 3281.385 kb on + strand, within Pf1N1B4_2923 at 3281.385 kb on + strand, within Pf1N1B4_2923 at 3281.385 kb on + strand, within Pf1N1B4_2923 at 3281.415 kb on + strand, within Pf1N1B4_2923 at 3281.415 kb on + strand, within Pf1N1B4_2923 at 3281.415 kb on + strand, within Pf1N1B4_2923 at 3281.563 kb on - strand, within Pf1N1B4_2923 at 3281.563 kb on - strand, within Pf1N1B4_2923 at 3281.563 kb on - strand, within Pf1N1B4_2923 at 3281.599 kb on + strand at 3281.599 kb on + strand at 3281.600 kb on - strand at 3281.600 kb on - strand at 3281.607 kb on + strand at 3281.607 kb on + strand at 3281.607 kb on + strand at 3281.607 kb on + strand at 3281.608 kb on - strand at 3281.608 kb on - strand at 3281.619 kb on + strand at 3281.619 kb on + strand at 3281.620 kb on - strand at 3281.634 kb on + strand at 3281.634 kb on + strand at 3281.634 kb on + strand at 3281.642 kb on - strand at 3281.652 kb on + strand at 3281.652 kb on + strand at 3281.652 kb on + strand at 3281.653 kb on - strand at 3281.653 kb on - strand at 3281.766 kb on + strand at 3281.766 kb on + strand at 3281.767 kb on - strand at 3281.864 kb on + strand, within Pf1N1B4_2924 at 3281.864 kb on + strand, within Pf1N1B4_2924 at 3281.865 kb on - strand, within Pf1N1B4_2924 at 3281.865 kb on - strand, within Pf1N1B4_2924 at 3281.865 kb on - strand, within Pf1N1B4_2924 at 3282.070 kb on - strand at 3282.070 kb on - strand at 3282.070 kb on - strand at 3282.070 kb on - strand at 3282.090 kb on + strand, within Pf1N1B4_2925 at 3282.090 kb on + strand, within Pf1N1B4_2925 at 3282.090 kb on + strand, within Pf1N1B4_2925 at 3282.091 kb on - strand at 3282.091 kb on - strand, within Pf1N1B4_2925 at 3282.091 kb on - strand, within Pf1N1B4_2925 at 3282.091 kb on - strand, within Pf1N1B4_2925 at 3282.297 kb on - strand, within Pf1N1B4_2926 at 3282.364 kb on - strand, within Pf1N1B4_2926 at 3282.379 kb on + strand, within Pf1N1B4_2926 at 3282.379 kb on + strand at 3282.461 kb on + strand, within Pf1N1B4_2926 at 3282.461 kb on + strand, within Pf1N1B4_2926 at 3282.488 kb on + strand, within Pf1N1B4_2926 at 3282.488 kb on + strand at 3282.488 kb on + strand, within Pf1N1B4_2926 at 3282.488 kb on + strand, within Pf1N1B4_2926 at 3282.489 kb on - strand, within Pf1N1B4_2926 at 3282.489 kb on - strand, within Pf1N1B4_2926 at 3282.489 kb on - strand, within Pf1N1B4_2926 at 3282.489 kb on - strand, within Pf1N1B4_2926 at 3282.566 kb on + strand, within Pf1N1B4_2926 at 3282.566 kb on + strand at 3282.566 kb on + strand, within Pf1N1B4_2926 at 3282.566 kb on + strand, within Pf1N1B4_2926 at 3282.566 kb on + strand, within Pf1N1B4_2926 at 3282.566 kb on + strand, within Pf1N1B4_2926 at 3282.567 kb on - strand, within Pf1N1B4_2926 at 3282.638 kb on + strand, within Pf1N1B4_2926 at 3282.638 kb on + strand, within Pf1N1B4_2926 at 3282.639 kb on - strand, within Pf1N1B4_2926 at 3282.906 kb on + strand at 3282.906 kb on + strand at 3282.907 kb on - strand at 3282.907 kb on - strand at 3282.907 kb on - strand at 3282.907 kb on - strand at 3282.907 kb on - strand at 3282.907 kb on - strand at 3282.907 kb on - strand at 3282.928 kb on + strand at 3282.928 kb on + strand at 3282.928 kb on + strand at 3282.928 kb on + strand at 3282.929 kb on - strand at 3282.929 kb on - strand at 3282.929 kb on - strand at 3282.929 kb on - strand at 3282.955 kb on + strand at 3282.955 kb on + strand at 3282.955 kb on + strand at 3282.955 kb on + strand at 3282.956 kb on - strand at 3282.956 kb on - strand at 3282.956 kb on - strand at 3282.959 kb on + strand at 3282.959 kb on + strand at 3282.960 kb on - strand at 3282.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 3,280,995 + -0.9 3,280,996 - +2.0 3,280,999 + -0.8 3,281,020 + +0.7 3,281,055 + +0.5 3,281,055 + -1.7 3,281,055 + +0.5 3,281,056 - +0.4 3,281,056 - +0.1 3,281,057 + +0.3 3,281,057 + +0.6 3,281,058 - +0.1 3,281,079 + +0.4 3,281,079 + -1.3 3,281,088 + -0.4 3,281,088 + -1.5 3,281,089 - -0.9 3,281,117 - -0.4 3,281,128 + +1.0 3,281,235 + Pf1N1B4_2923 0.23 +0.2 3,281,319 + Pf1N1B4_2923 0.39 -0.9 3,281,319 + Pf1N1B4_2923 0.39 +1.1 3,281,319 + Pf1N1B4_2923 0.39 +0.3 3,281,319 + Pf1N1B4_2923 0.39 +0.2 3,281,319 + Pf1N1B4_2923 0.39 -1.0 3,281,319 + Pf1N1B4_2923 0.39 +0.8 3,281,320 - Pf1N1B4_2923 0.39 +0.9 3,281,325 + Pf1N1B4_2923 0.40 +0.7 3,281,325 + Pf1N1B4_2923 0.40 +0.2 3,281,385 + Pf1N1B4_2923 0.51 +0.8 3,281,385 + Pf1N1B4_2923 0.51 -0.6 3,281,385 + Pf1N1B4_2923 0.51 -0.6 3,281,415 + Pf1N1B4_2923 0.57 +0.2 3,281,415 + Pf1N1B4_2923 0.57 +0.1 3,281,415 + Pf1N1B4_2923 0.57 -0.5 3,281,563 - Pf1N1B4_2923 0.86 -0.1 3,281,563 - Pf1N1B4_2923 0.86 +0.2 3,281,563 - Pf1N1B4_2923 0.86 +1.6 3,281,599 + -2.3 3,281,599 + +0.3 3,281,600 - -1.0 3,281,600 - +0.4 3,281,607 + +0.6 3,281,607 + -0.2 3,281,607 + -0.1 3,281,607 + -0.2 3,281,608 - -0.1 3,281,608 - +0.8 3,281,619 + +0.7 3,281,619 + +0.0 3,281,620 - -0.4 3,281,634 + +1.1 3,281,634 + -0.1 3,281,634 + -0.3 3,281,642 - +0.0 3,281,652 + +0.2 3,281,652 + +0.7 3,281,652 + +1.1 3,281,653 - +0.5 3,281,653 - -2.0 3,281,766 + +0.6 3,281,766 + -0.9 3,281,767 - -1.8 3,281,864 + Pf1N1B4_2924 0.30 -1.1 3,281,864 + Pf1N1B4_2924 0.30 +0.2 3,281,865 - Pf1N1B4_2924 0.30 -2.2 3,281,865 - Pf1N1B4_2924 0.30 -0.4 3,281,865 - Pf1N1B4_2924 0.30 -0.6 3,282,070 - -2.3 3,282,070 - +0.0 3,282,070 - +0.3 3,282,070 - +0.2 3,282,090 + Pf1N1B4_2925 0.79 -1.2 3,282,090 + Pf1N1B4_2925 0.79 -0.0 3,282,090 + Pf1N1B4_2925 0.79 -0.8 3,282,091 - +1.9 3,282,091 - Pf1N1B4_2925 0.80 -0.0 3,282,091 - Pf1N1B4_2925 0.80 -0.6 3,282,091 - Pf1N1B4_2925 0.80 -0.3 3,282,297 - Pf1N1B4_2926 0.21 -0.1 3,282,364 - Pf1N1B4_2926 0.30 +0.5 3,282,379 + Pf1N1B4_2926 0.32 -1.3 3,282,379 + -0.2 3,282,461 + Pf1N1B4_2926 0.43 +1.1 3,282,461 + Pf1N1B4_2926 0.43 +0.5 3,282,488 + Pf1N1B4_2926 0.47 -0.9 3,282,488 + -1.6 3,282,488 + Pf1N1B4_2926 0.47 +0.3 3,282,488 + Pf1N1B4_2926 0.47 -0.9 3,282,489 - Pf1N1B4_2926 0.47 -0.5 3,282,489 - Pf1N1B4_2926 0.47 -0.6 3,282,489 - Pf1N1B4_2926 0.47 -0.5 3,282,489 - Pf1N1B4_2926 0.47 -1.1 3,282,566 + Pf1N1B4_2926 0.57 +0.8 3,282,566 + -0.9 3,282,566 + Pf1N1B4_2926 0.57 +0.4 3,282,566 + Pf1N1B4_2926 0.57 +0.3 3,282,566 + Pf1N1B4_2926 0.57 -0.1 3,282,566 + Pf1N1B4_2926 0.57 +0.2 3,282,567 - Pf1N1B4_2926 0.57 +0.3 3,282,638 + Pf1N1B4_2926 0.67 -0.4 3,282,638 + Pf1N1B4_2926 0.67 -1.3 3,282,639 - Pf1N1B4_2926 0.67 +0.7 3,282,906 + +1.6 3,282,906 + -0.1 3,282,907 - +0.1 3,282,907 - +0.4 3,282,907 - +0.4 3,282,907 - +0.4 3,282,907 - -0.0 3,282,907 - -0.1 3,282,907 - +0.9 3,282,928 + -0.2 3,282,928 + -0.5 3,282,928 + -2.0 3,282,928 + -1.3 3,282,929 - -1.8 3,282,929 - +0.5 3,282,929 - -1.3 3,282,929 - -1.3 3,282,955 + -1.1 3,282,955 + -0.2 3,282,955 + -0.5 3,282,955 + +1.0 3,282,956 - +0.1 3,282,956 - +0.4 3,282,956 - -0.3 3,282,959 + -0.7 3,282,959 + -0.6 3,282,960 - -0.7 3,282,960 - -0.5
Or see this region's nucleotide sequence