Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2169 and Pf1N1B4_2170 are separated by 162 nucleotides Pf1N1B4_2170 and Pf1N1B4_2171 overlap by 4 nucleotides Pf1N1B4_2171 and Pf1N1B4_2172 are separated by 2 nucleotides Pf1N1B4_2172 and Pf1N1B4_2173 are separated by 13 nucleotides
Pf1N1B4_2169 - FIG00953808: hypothetical protein, at 2,483,135 to 2,483,458
_2169
Pf1N1B4_2170 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 2,483,621 to 2,484,322
_2170
Pf1N1B4_2171 - Tyrosine recombinase XerC, at 2,484,319 to 2,485,218
_2171
Pf1N1B4_2172 - Protein of unknown function DUF484, at 2,485,221 to 2,485,946
_2172
Pf1N1B4_2173 - Diaminopimelate epimerase (EC 5.1.1.7), at 2,485,960 to 2,486,790
_2173
Position (kb)
2484
2485
2486 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2483.432 kb on - strand at 2483.438 kb on + strand at 2483.439 kb on - strand at 2483.439 kb on - strand at 2483.439 kb on - strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.558 kb on + strand at 2483.558 kb on + strand at 2483.558 kb on + strand at 2483.559 kb on - strand at 2483.559 kb on - strand at 2483.569 kb on + strand at 2483.569 kb on + strand at 2483.569 kb on + strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.577 kb on + strand at 2483.578 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.629 kb on + strand at 2483.629 kb on + strand at 2483.630 kb on - strand at 2483.630 kb on - strand at 2483.668 kb on + strand at 2483.705 kb on + strand, within Pf1N1B4_2170 at 2483.706 kb on - strand, within Pf1N1B4_2170 at 2483.788 kb on + strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.899 kb on - strand, within Pf1N1B4_2170 at 2483.951 kb on + strand, within Pf1N1B4_2170 at 2483.952 kb on - strand, within Pf1N1B4_2170 at 2483.952 kb on - strand, within Pf1N1B4_2170 at 2483.958 kb on + strand, within Pf1N1B4_2170 at 2483.958 kb on + strand, within Pf1N1B4_2170 at 2484.070 kb on + strand, within Pf1N1B4_2170 at 2484.070 kb on + strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.336 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.797 kb on + strand, within Pf1N1B4_2171 at 2484.798 kb on - strand, within Pf1N1B4_2171 at 2484.798 kb on - strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.977 kb on + strand, within Pf1N1B4_2171 at 2484.977 kb on + strand, within Pf1N1B4_2171 at 2484.978 kb on - strand, within Pf1N1B4_2171 at 2485.148 kb on + strand at 2485.227 kb on + strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.319 kb on - strand at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.626 kb on + strand, within Pf1N1B4_2172 at 2485.626 kb on + strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.918 kb on - strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.983 kb on - strand at 2485.983 kb on - strand at 2485.983 kb on - strand at 2486.071 kb on + strand, within Pf1N1B4_2173
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 2,483,432 - -0.7 2,483,438 + -0.3 2,483,439 - -0.2 2,483,439 - +0.8 2,483,439 - -1.7 2,483,452 + +0.2 2,483,452 + +0.8 2,483,452 + -1.3 2,483,452 + -0.4 2,483,453 - +2.5 2,483,453 - -0.2 2,483,453 - -0.4 2,483,453 - +0.3 2,483,456 + +0.4 2,483,456 + +0.3 2,483,456 + -1.0 2,483,456 + -0.3 2,483,456 + +2.3 2,483,456 + -1.3 2,483,456 + +0.1 2,483,456 + -1.7 2,483,456 + +0.3 2,483,456 + -0.5 2,483,456 + -0.8 2,483,456 + +0.8 2,483,457 - +0.1 2,483,457 - -0.0 2,483,457 - +0.3 2,483,457 - -0.6 2,483,457 - -1.8 2,483,457 - +0.6 2,483,457 - +0.4 2,483,457 - -0.0 2,483,457 - +0.2 2,483,457 - +0.9 2,483,457 - +0.7 2,483,553 + +0.2 2,483,553 + +0.0 2,483,553 + +0.2 2,483,553 + +0.8 2,483,554 - +0.5 2,483,554 - -0.2 2,483,554 - -2.3 2,483,554 - -2.0 2,483,554 - +0.2 2,483,554 - +0.3 2,483,554 - +1.0 2,483,558 + +0.2 2,483,558 + +1.5 2,483,558 + -0.0 2,483,559 - -1.9 2,483,559 - +1.7 2,483,569 + -1.0 2,483,569 + +1.4 2,483,569 + +0.3 2,483,570 - +0.4 2,483,570 - -0.1 2,483,570 - -0.0 2,483,570 - -0.3 2,483,570 - -0.3 2,483,570 - -0.1 2,483,570 - +1.0 2,483,577 + -1.9 2,483,578 - +0.2 2,483,623 - -0.7 2,483,623 - -1.4 2,483,623 - -0.4 2,483,623 - -0.0 2,483,629 + +0.4 2,483,629 + +1.6 2,483,630 - -0.9 2,483,630 - -1.2 2,483,668 + -1.5 2,483,705 + Pf1N1B4_2170 0.12 -0.2 2,483,706 - Pf1N1B4_2170 0.12 +1.2 2,483,788 + Pf1N1B4_2170 0.24 -0.8 2,483,789 - Pf1N1B4_2170 0.24 +0.3 2,483,789 - Pf1N1B4_2170 0.24 -0.3 2,483,789 - Pf1N1B4_2170 0.24 -1.4 2,483,789 - Pf1N1B4_2170 0.24 -1.0 2,483,899 - Pf1N1B4_2170 0.40 +0.3 2,483,951 + Pf1N1B4_2170 0.47 +0.6 2,483,952 - Pf1N1B4_2170 0.47 +0.1 2,483,952 - Pf1N1B4_2170 0.47 +1.2 2,483,958 + Pf1N1B4_2170 0.48 -2.2 2,483,958 + Pf1N1B4_2170 0.48 +0.6 2,484,070 + Pf1N1B4_2170 0.64 -0.1 2,484,070 + Pf1N1B4_2170 0.64 +0.5 2,484,071 - Pf1N1B4_2170 0.64 -2.3 2,484,071 - Pf1N1B4_2170 0.64 -0.1 2,484,071 - Pf1N1B4_2170 0.64 +0.8 2,484,071 - Pf1N1B4_2170 0.64 -0.2 2,484,071 - Pf1N1B4_2170 0.64 -0.6 2,484,336 + -0.4 2,484,368 + -0.5 2,484,368 + -0.2 2,484,368 + -0.8 2,484,404 + +0.2 2,484,404 + -0.8 2,484,404 + -1.1 2,484,404 + +0.8 2,484,404 + +0.5 2,484,405 - -1.8 2,484,405 - -0.1 2,484,405 - +0.6 2,484,797 + Pf1N1B4_2171 0.53 -0.4 2,484,798 - Pf1N1B4_2171 0.53 -0.7 2,484,798 - Pf1N1B4_2171 0.53 -0.8 2,484,967 + Pf1N1B4_2171 0.72 -0.6 2,484,967 + Pf1N1B4_2171 0.72 +0.3 2,484,967 + Pf1N1B4_2171 0.72 -0.7 2,484,967 + Pf1N1B4_2171 0.72 +0.5 2,484,977 + Pf1N1B4_2171 0.73 -1.5 2,484,977 + Pf1N1B4_2171 0.73 -1.0 2,484,978 - Pf1N1B4_2171 0.73 -0.9 2,485,148 + -0.3 2,485,227 + +0.3 2,485,286 - +0.6 2,485,286 - -1.9 2,485,286 - -0.6 2,485,286 - -0.8 2,485,286 - -0.8 2,485,318 + Pf1N1B4_2172 0.13 +0.2 2,485,318 + Pf1N1B4_2172 0.13 -0.4 2,485,318 + Pf1N1B4_2172 0.13 +1.2 2,485,318 + Pf1N1B4_2172 0.13 -2.0 2,485,319 - +0.2 2,485,319 - Pf1N1B4_2172 0.13 -0.3 2,485,319 - Pf1N1B4_2172 0.13 +0.5 2,485,319 - Pf1N1B4_2172 0.13 -0.0 2,485,626 + Pf1N1B4_2172 0.56 +0.4 2,485,626 + Pf1N1B4_2172 0.56 +0.3 2,485,627 - Pf1N1B4_2172 0.56 +0.7 2,485,627 - Pf1N1B4_2172 0.56 -0.3 2,485,627 - Pf1N1B4_2172 0.56 +0.8 2,485,821 + Pf1N1B4_2172 0.83 -2.5 2,485,821 + Pf1N1B4_2172 0.83 +0.1 2,485,821 + Pf1N1B4_2172 0.83 -0.2 2,485,822 - Pf1N1B4_2172 0.83 -1.4 2,485,822 - Pf1N1B4_2172 0.83 -1.6 2,485,822 - Pf1N1B4_2172 0.83 -1.4 2,485,918 - -0.5 2,485,982 + +0.3 2,485,982 + +0.8 2,485,982 + -1.3 2,485,982 + +0.0 2,485,983 - -0.4 2,485,983 - -0.9 2,485,983 - +1.1 2,486,071 + Pf1N1B4_2173 0.13 +0.5
Or see this region's nucleotide sequence