Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_464 and Pf1N1B4_465 overlap by 4 nucleotides Pf1N1B4_465 and Pf1N1B4_466 are separated by 232 nucleotides
Pf1N1B4_464 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 555,443 to 557,764
_464
Pf1N1B4_465 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 557,761 to 558,234
_465
Pf1N1B4_466 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 558,467 to 559,486
_466
Position (kb) 557
558
559 Strain fitness (log2 ratio) -2
-1
0
1
2 at 556.818 kb on + strand, within Pf1N1B4_464 at 556.818 kb on + strand, within Pf1N1B4_464 at 556.819 kb on - strand, within Pf1N1B4_464 at 556.885 kb on + strand, within Pf1N1B4_464 at 556.885 kb on + strand, within Pf1N1B4_464 at 556.885 kb on + strand, within Pf1N1B4_464 at 557.083 kb on - strand, within Pf1N1B4_464 at 557.266 kb on + strand, within Pf1N1B4_464 at 557.266 kb on + strand, within Pf1N1B4_464 at 557.370 kb on + strand, within Pf1N1B4_464 at 557.574 kb on + strand at 557.574 kb on + strand at 557.575 kb on - strand at 557.575 kb on - strand at 557.575 kb on - strand at 557.582 kb on - strand at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand, within Pf1N1B4_465 at 557.823 kb on - strand, within Pf1N1B4_465 at 557.823 kb on - strand, within Pf1N1B4_465 at 558.013 kb on + strand at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.019 kb on - strand, within Pf1N1B4_465 at 558.093 kb on + strand, within Pf1N1B4_465 at 558.093 kb on + strand, within Pf1N1B4_465 at 558.190 kb on + strand at 558.191 kb on - strand at 558.203 kb on + strand at 558.203 kb on + strand at 558.204 kb on - strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.229 kb on - strand at 558.229 kb on - strand at 558.229 kb on - strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.249 kb on - strand at 558.249 kb on - strand at 558.249 kb on - strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.255 kb on - strand at 558.255 kb on - strand at 558.255 kb on - strand at 558.326 kb on - strand at 558.388 kb on + strand at 558.393 kb on + strand at 558.393 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 556,818 + Pf1N1B4_464 0.59 -1.7 556,818 + Pf1N1B4_464 0.59 +1.5 556,819 - Pf1N1B4_464 0.59 -0.5 556,885 + Pf1N1B4_464 0.62 -0.1 556,885 + Pf1N1B4_464 0.62 +0.2 556,885 + Pf1N1B4_464 0.62 -0.5 557,083 - Pf1N1B4_464 0.71 +0.2 557,266 + Pf1N1B4_464 0.79 +0.1 557,266 + Pf1N1B4_464 0.79 -0.9 557,370 + Pf1N1B4_464 0.83 +0.2 557,574 + +0.7 557,574 + -0.5 557,575 - +0.5 557,575 - -0.6 557,575 - -0.7 557,582 - -0.1 557,822 + Pf1N1B4_465 0.13 -0.2 557,822 + Pf1N1B4_465 0.13 -2.4 557,822 + +0.9 557,822 + Pf1N1B4_465 0.13 -0.6 557,822 + Pf1N1B4_465 0.13 +0.5 557,823 - Pf1N1B4_465 0.13 -0.9 557,823 - Pf1N1B4_465 0.13 +1.2 558,013 + +0.3 558,013 + Pf1N1B4_465 0.53 +1.1 558,013 + Pf1N1B4_465 0.53 +0.7 558,013 + Pf1N1B4_465 0.53 +0.1 558,013 + Pf1N1B4_465 0.53 +0.2 558,013 + Pf1N1B4_465 0.53 -1.9 558,013 + Pf1N1B4_465 0.53 -0.1 558,014 - Pf1N1B4_465 0.53 +0.3 558,014 - Pf1N1B4_465 0.53 +1.0 558,014 - Pf1N1B4_465 0.53 +2.5 558,014 - -0.7 558,014 - Pf1N1B4_465 0.53 +0.5 558,014 - Pf1N1B4_465 0.53 +0.4 558,014 - Pf1N1B4_465 0.53 +1.1 558,014 - Pf1N1B4_465 0.53 +0.5 558,014 - Pf1N1B4_465 0.53 -0.4 558,019 - Pf1N1B4_465 0.54 +0.5 558,093 + Pf1N1B4_465 0.70 -1.1 558,093 + Pf1N1B4_465 0.70 +0.1 558,190 + -0.5 558,191 - -0.2 558,203 + -0.6 558,203 + +0.5 558,204 - +0.7 558,228 + +1.7 558,228 + +0.8 558,228 + -0.7 558,228 + +0.3 558,228 + -1.8 558,229 - +0.1 558,229 - +1.1 558,229 - -0.2 558,248 + +0.2 558,248 + +0.6 558,248 + -0.7 558,248 + -0.1 558,248 + +0.6 558,248 + +0.1 558,249 - +0.0 558,249 - -0.8 558,249 - +0.2 558,252 + -0.0 558,252 + +0.9 558,252 + +0.3 558,252 + -0.2 558,252 + -0.8 558,252 + -2.7 558,252 + -0.2 558,252 + +0.0 558,253 - +1.0 558,253 - -0.3 558,253 - -0.2 558,253 - +0.1 558,253 - -1.2 558,254 + -0.5 558,254 + +0.1 558,254 + +0.7 558,254 + -1.4 558,254 + +0.1 558,254 + +0.1 558,254 + +0.5 558,255 - -0.1 558,255 - +0.6 558,255 - -1.2 558,326 - +1.2 558,388 + -0.7 558,393 + +0.3 558,393 + -1.1
Or see this region's nucleotide sequence