Experiment: Minimal media pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1644 and Pf1N1B4_1645 are separated by 202 nucleotides Pf1N1B4_1645 and Pf1N1B4_1646 are separated by 30 nucleotides Pf1N1B4_1646 and Pf1N1B4_5185 are separated by 78 nucleotides
Pf1N1B4_1644 - SAM-dependent methyltransferases, at 1,886,800 to 1,888,002
_1644
Pf1N1B4_1645 - hypothetical protein, at 1,888,205 to 1,888,318
_1645
Pf1N1B4_1646 - SAM-dependent methyltransferases, at 1,888,349 to 1,889,170
_1646
Pf1N1B4_5185 - tRNA-Thr-TGT, at 1,889,249 to 1,889,321
_5185
Position (kb)
1888
1889 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1887.362 kb on + strand at 1887.362 kb on + strand, within Pf1N1B4_1644 at 1887.363 kb on - strand, within Pf1N1B4_1644 at 1887.478 kb on + strand, within Pf1N1B4_1644 at 1887.478 kb on + strand, within Pf1N1B4_1644 at 1887.502 kb on + strand, within Pf1N1B4_1644 at 1887.502 kb on + strand, within Pf1N1B4_1644 at 1887.502 kb on + strand, within Pf1N1B4_1644 at 1887.502 kb on + strand, within Pf1N1B4_1644 at 1887.502 kb on + strand, within Pf1N1B4_1644 at 1887.503 kb on - strand, within Pf1N1B4_1644 at 1887.503 kb on - strand, within Pf1N1B4_1644 at 1887.503 kb on - strand, within Pf1N1B4_1644 at 1887.503 kb on - strand, within Pf1N1B4_1644 at 1887.503 kb on - strand, within Pf1N1B4_1644 at 1887.503 kb on - strand, within Pf1N1B4_1644 at 1887.539 kb on + strand, within Pf1N1B4_1644 at 1887.539 kb on + strand, within Pf1N1B4_1644 at 1887.539 kb on + strand, within Pf1N1B4_1644 at 1887.540 kb on - strand, within Pf1N1B4_1644 at 1887.540 kb on - strand, within Pf1N1B4_1644 at 1887.540 kb on - strand, within Pf1N1B4_1644 at 1887.540 kb on - strand, within Pf1N1B4_1644 at 1887.682 kb on + strand, within Pf1N1B4_1644 at 1887.682 kb on + strand, within Pf1N1B4_1644 at 1887.682 kb on + strand, within Pf1N1B4_1644 at 1887.767 kb on + strand, within Pf1N1B4_1644 at 1887.767 kb on + strand at 1887.795 kb on + strand, within Pf1N1B4_1644 at 1887.795 kb on + strand, within Pf1N1B4_1644 at 1887.852 kb on - strand, within Pf1N1B4_1644 at 1887.852 kb on - strand, within Pf1N1B4_1644 at 1887.891 kb on - strand at 1887.891 kb on - strand at 1887.891 kb on - strand at 1887.925 kb on + strand at 1887.981 kb on - strand at 1887.981 kb on - strand at 1887.981 kb on - strand at 1888.000 kb on + strand at 1888.000 kb on + strand at 1888.000 kb on + strand at 1888.000 kb on + strand at 1888.001 kb on - strand at 1888.001 kb on - strand at 1888.001 kb on - strand at 1888.022 kb on - strand at 1888.068 kb on - strand at 1888.068 kb on - strand at 1888.198 kb on + strand at 1888.198 kb on + strand at 1888.199 kb on - strand at 1888.199 kb on - strand at 1888.199 kb on - strand at 1888.207 kb on - strand at 1888.207 kb on - strand at 1888.277 kb on + strand, within Pf1N1B4_1645 at 1888.278 kb on - strand, within Pf1N1B4_1645 at 1888.278 kb on - strand, within Pf1N1B4_1645 at 1888.278 kb on - strand, within Pf1N1B4_1645 at 1888.278 kb on - strand, within Pf1N1B4_1645 at 1888.279 kb on + strand, within Pf1N1B4_1645 at 1888.279 kb on + strand, within Pf1N1B4_1645 at 1888.279 kb on + strand, within Pf1N1B4_1645 at 1888.279 kb on + strand, within Pf1N1B4_1645 at 1888.279 kb on + strand, within Pf1N1B4_1645 at 1888.279 kb on + strand, within Pf1N1B4_1645 at 1888.280 kb on - strand, within Pf1N1B4_1645 at 1888.280 kb on - strand, within Pf1N1B4_1645 at 1888.280 kb on - strand, within Pf1N1B4_1645 at 1888.280 kb on - strand, within Pf1N1B4_1645 at 1888.280 kb on - strand, within Pf1N1B4_1645 at 1888.280 kb on - strand, within Pf1N1B4_1645 at 1888.280 kb on - strand, within Pf1N1B4_1645 at 1888.280 kb on - strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.287 kb on + strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.288 kb on - strand, within Pf1N1B4_1645 at 1888.406 kb on + strand at 1888.406 kb on + strand at 1888.407 kb on - strand at 1888.407 kb on - strand at 1888.424 kb on + strand at 1888.424 kb on + strand at 1888.424 kb on + strand at 1888.424 kb on + strand at 1888.424 kb on + strand at 1888.424 kb on + strand at 1888.424 kb on + strand at 1888.424 kb on + strand at 1888.425 kb on - strand at 1888.425 kb on - strand at 1888.425 kb on - strand at 1888.425 kb on - strand at 1888.478 kb on + strand, within Pf1N1B4_1646 at 1888.478 kb on + strand, within Pf1N1B4_1646 at 1888.479 kb on - strand, within Pf1N1B4_1646 at 1888.479 kb on - strand, within Pf1N1B4_1646 at 1888.479 kb on - strand, within Pf1N1B4_1646 at 1888.479 kb on - strand, within Pf1N1B4_1646 at 1888.479 kb on - strand, within Pf1N1B4_1646 at 1888.523 kb on + strand at 1888.524 kb on - strand, within Pf1N1B4_1646 at 1888.527 kb on - strand, within Pf1N1B4_1646 at 1888.607 kb on + strand, within Pf1N1B4_1646 at 1888.607 kb on + strand, within Pf1N1B4_1646 at 1888.607 kb on + strand, within Pf1N1B4_1646 at 1888.608 kb on - strand, within Pf1N1B4_1646 at 1888.608 kb on - strand, within Pf1N1B4_1646 at 1888.608 kb on - strand, within Pf1N1B4_1646 at 1888.608 kb on - strand, within Pf1N1B4_1646 at 1888.931 kb on + strand, within Pf1N1B4_1646 at 1888.931 kb on + strand, within Pf1N1B4_1646 at 1888.931 kb on + strand, within Pf1N1B4_1646 at 1888.931 kb on + strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.932 kb on - strand, within Pf1N1B4_1646 at 1888.934 kb on + strand, within Pf1N1B4_1646 at 1888.934 kb on + strand at 1888.934 kb on + strand, within Pf1N1B4_1646 at 1888.935 kb on - strand, within Pf1N1B4_1646 at 1888.935 kb on - strand, within Pf1N1B4_1646 at 1888.935 kb on - strand, within Pf1N1B4_1646 at 1889.185 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH6 remove 1,887,362 + +1.1 1,887,362 + Pf1N1B4_1644 0.47 -1.3 1,887,363 - Pf1N1B4_1644 0.47 -0.8 1,887,478 + Pf1N1B4_1644 0.56 -0.1 1,887,478 + Pf1N1B4_1644 0.56 -0.1 1,887,502 + Pf1N1B4_1644 0.58 -0.2 1,887,502 + Pf1N1B4_1644 0.58 +0.3 1,887,502 + Pf1N1B4_1644 0.58 -0.1 1,887,502 + Pf1N1B4_1644 0.58 -0.1 1,887,502 + Pf1N1B4_1644 0.58 +0.0 1,887,503 - Pf1N1B4_1644 0.58 +0.6 1,887,503 - Pf1N1B4_1644 0.58 +0.6 1,887,503 - Pf1N1B4_1644 0.58 -0.5 1,887,503 - Pf1N1B4_1644 0.58 -1.4 1,887,503 - Pf1N1B4_1644 0.58 -1.5 1,887,503 - Pf1N1B4_1644 0.58 +1.5 1,887,539 + Pf1N1B4_1644 0.61 +0.8 1,887,539 + Pf1N1B4_1644 0.61 +0.9 1,887,539 + Pf1N1B4_1644 0.61 +0.1 1,887,540 - Pf1N1B4_1644 0.62 -0.3 1,887,540 - Pf1N1B4_1644 0.62 -0.0 1,887,540 - Pf1N1B4_1644 0.62 +0.9 1,887,540 - Pf1N1B4_1644 0.62 -0.2 1,887,682 + Pf1N1B4_1644 0.73 -0.9 1,887,682 + Pf1N1B4_1644 0.73 +0.1 1,887,682 + Pf1N1B4_1644 0.73 -0.0 1,887,767 + Pf1N1B4_1644 0.80 +1.0 1,887,767 + -1.3 1,887,795 + Pf1N1B4_1644 0.83 +0.3 1,887,795 + Pf1N1B4_1644 0.83 +0.1 1,887,852 - Pf1N1B4_1644 0.87 -0.0 1,887,852 - Pf1N1B4_1644 0.87 -0.1 1,887,891 - -2.5 1,887,891 - -1.0 1,887,891 - -2.2 1,887,925 + +0.7 1,887,981 - +0.9 1,887,981 - +0.6 1,887,981 - +1.8 1,888,000 + -1.3 1,888,000 + +2.7 1,888,000 + +0.2 1,888,000 + +0.2 1,888,001 - -1.1 1,888,001 - -0.0 1,888,001 - +1.2 1,888,022 - +0.2 1,888,068 - -0.9 1,888,068 - +1.3 1,888,198 + +0.3 1,888,198 + +1.2 1,888,199 - -0.2 1,888,199 - +0.6 1,888,199 - +0.1 1,888,207 - +0.3 1,888,207 - -1.8 1,888,277 + Pf1N1B4_1645 0.63 +0.4 1,888,278 - Pf1N1B4_1645 0.64 -0.4 1,888,278 - Pf1N1B4_1645 0.64 +0.6 1,888,278 - Pf1N1B4_1645 0.64 +0.0 1,888,278 - Pf1N1B4_1645 0.64 -0.3 1,888,279 + Pf1N1B4_1645 0.65 -0.9 1,888,279 + Pf1N1B4_1645 0.65 +0.0 1,888,279 + Pf1N1B4_1645 0.65 -1.1 1,888,279 + Pf1N1B4_1645 0.65 +0.5 1,888,279 + Pf1N1B4_1645 0.65 +0.6 1,888,279 + Pf1N1B4_1645 0.65 -0.9 1,888,280 - Pf1N1B4_1645 0.66 +0.4 1,888,280 - Pf1N1B4_1645 0.66 -0.1 1,888,280 - Pf1N1B4_1645 0.66 +0.3 1,888,280 - Pf1N1B4_1645 0.66 -1.5 1,888,280 - Pf1N1B4_1645 0.66 +1.0 1,888,280 - Pf1N1B4_1645 0.66 -0.1 1,888,280 - Pf1N1B4_1645 0.66 -0.1 1,888,280 - Pf1N1B4_1645 0.66 -0.6 1,888,287 + Pf1N1B4_1645 0.72 -0.5 1,888,287 + Pf1N1B4_1645 0.72 +0.5 1,888,287 + Pf1N1B4_1645 0.72 -0.1 1,888,287 + Pf1N1B4_1645 0.72 +0.6 1,888,287 + Pf1N1B4_1645 0.72 -0.0 1,888,287 + Pf1N1B4_1645 0.72 -0.4 1,888,287 + Pf1N1B4_1645 0.72 +0.2 1,888,287 + Pf1N1B4_1645 0.72 +0.5 1,888,287 + Pf1N1B4_1645 0.72 -1.5 1,888,287 + Pf1N1B4_1645 0.72 -0.4 1,888,287 + Pf1N1B4_1645 0.72 -0.2 1,888,287 + Pf1N1B4_1645 0.72 +0.9 1,888,288 - Pf1N1B4_1645 0.73 -0.5 1,888,288 - Pf1N1B4_1645 0.73 -0.1 1,888,288 - Pf1N1B4_1645 0.73 -2.2 1,888,288 - Pf1N1B4_1645 0.73 +0.5 1,888,288 - Pf1N1B4_1645 0.73 +1.5 1,888,288 - Pf1N1B4_1645 0.73 +0.5 1,888,288 - Pf1N1B4_1645 0.73 -0.6 1,888,288 - Pf1N1B4_1645 0.73 -0.6 1,888,288 - Pf1N1B4_1645 0.73 -0.0 1,888,288 - Pf1N1B4_1645 0.73 +1.2 1,888,288 - Pf1N1B4_1645 0.73 +0.4 1,888,406 + -1.2 1,888,406 + +1.2 1,888,407 - -0.7 1,888,407 - +1.1 1,888,424 + -0.5 1,888,424 + +0.1 1,888,424 + -0.0 1,888,424 + -1.0 1,888,424 + -1.7 1,888,424 + +0.4 1,888,424 + -0.9 1,888,424 + +2.1 1,888,425 - +0.3 1,888,425 - -0.8 1,888,425 - -0.0 1,888,425 - -0.3 1,888,478 + Pf1N1B4_1646 0.16 +1.0 1,888,478 + Pf1N1B4_1646 0.16 +1.2 1,888,479 - Pf1N1B4_1646 0.16 -0.3 1,888,479 - Pf1N1B4_1646 0.16 -0.0 1,888,479 - Pf1N1B4_1646 0.16 +0.1 1,888,479 - Pf1N1B4_1646 0.16 +0.5 1,888,479 - Pf1N1B4_1646 0.16 -0.0 1,888,523 + +1.5 1,888,524 - Pf1N1B4_1646 0.21 -0.1 1,888,527 - Pf1N1B4_1646 0.22 +0.9 1,888,607 + Pf1N1B4_1646 0.31 -1.9 1,888,607 + Pf1N1B4_1646 0.31 +0.0 1,888,607 + Pf1N1B4_1646 0.31 +1.4 1,888,608 - Pf1N1B4_1646 0.32 -1.4 1,888,608 - Pf1N1B4_1646 0.32 -0.7 1,888,608 - Pf1N1B4_1646 0.32 -1.6 1,888,608 - Pf1N1B4_1646 0.32 +1.0 1,888,931 + Pf1N1B4_1646 0.71 -0.9 1,888,931 + Pf1N1B4_1646 0.71 +0.4 1,888,931 + Pf1N1B4_1646 0.71 -0.8 1,888,931 + Pf1N1B4_1646 0.71 -2.9 1,888,932 - Pf1N1B4_1646 0.71 +1.8 1,888,932 - Pf1N1B4_1646 0.71 -0.8 1,888,932 - Pf1N1B4_1646 0.71 +1.2 1,888,932 - Pf1N1B4_1646 0.71 -0.4 1,888,932 - Pf1N1B4_1646 0.71 +1.4 1,888,932 - Pf1N1B4_1646 0.71 +1.3 1,888,932 - Pf1N1B4_1646 0.71 -0.8 1,888,932 - Pf1N1B4_1646 0.71 -0.3 1,888,932 - Pf1N1B4_1646 0.71 -0.3 1,888,932 - Pf1N1B4_1646 0.71 -1.6 1,888,932 - Pf1N1B4_1646 0.71 -0.2 1,888,932 - Pf1N1B4_1646 0.71 +1.7 1,888,932 - Pf1N1B4_1646 0.71 +0.8 1,888,932 - Pf1N1B4_1646 0.71 -1.0 1,888,932 - Pf1N1B4_1646 0.71 +0.9 1,888,934 + Pf1N1B4_1646 0.71 -0.1 1,888,934 + -0.0 1,888,934 + Pf1N1B4_1646 0.71 -0.3 1,888,935 - Pf1N1B4_1646 0.71 +1.3 1,888,935 - Pf1N1B4_1646 0.71 +0.8 1,888,935 - Pf1N1B4_1646 0.71 -0.2 1,889,185 - -0.1
Or see this region's nucleotide sequence