Experiment: ALP with nitrate 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1599 and Dsui_1600 overlap by 4 nucleotides Dsui_1600 and Dsui_1601 are separated by 27 nucleotides Dsui_1601 and Dsui_1602 are separated by 142 nucleotides
Dsui_1599: Dsui_1599 - putative enzyme of heme biosynthesis, at 1,679,632 to 1,680,834
_1599
Dsui_1600: Dsui_1600 - putative enzyme of heme biosynthesis, at 1,680,831 to 1,681,916
_1600
Dsui_1601: Dsui_1601 - uroporphyrinogen-III synthase, at 1,681,944 to 1,682,711
_1601
Dsui_1602: Dsui_1602 - porphobilinogen deaminase, at 1,682,854 to 1,683,813
_1602
Position (kb)
1680
1681
1682 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1680.002 kb on + strand, within Dsui_1599 at 1680.010 kb on - strand, within Dsui_1599 at 1680.102 kb on + strand, within Dsui_1599 at 1680.251 kb on + strand, within Dsui_1599 at 1680.366 kb on + strand, within Dsui_1599 at 1680.389 kb on - strand, within Dsui_1599 at 1680.394 kb on + strand, within Dsui_1599 at 1680.584 kb on + strand, within Dsui_1599 at 1680.592 kb on - strand, within Dsui_1599 at 1680.769 kb on - strand at 1680.849 kb on - strand at 1680.851 kb on + strand at 1680.859 kb on - strand at 1680.861 kb on + strand at 1680.868 kb on + strand at 1681.078 kb on - strand, within Dsui_1600 at 1681.090 kb on + strand, within Dsui_1600 at 1681.113 kb on - strand, within Dsui_1600 at 1681.131 kb on + strand at 1681.189 kb on - strand, within Dsui_1600 at 1681.386 kb on - strand, within Dsui_1600 at 1681.471 kb on + strand, within Dsui_1600 at 1681.504 kb on - strand, within Dsui_1600 at 1681.504 kb on - strand, within Dsui_1600 at 1681.513 kb on + strand at 1681.518 kb on + strand at 1681.656 kb on - strand, within Dsui_1600 at 1681.783 kb on - strand, within Dsui_1600 at 1681.890 kb on - strand at 1681.919 kb on - strand at 1681.930 kb on - strand at 1682.738 kb on + strand at 1682.762 kb on + strand at 1682.774 kb on - strand at 1682.779 kb on + strand at 1682.846 kb on - strand at 1682.859 kb on + strand at 1682.865 kb on - strand at 1682.869 kb on + strand at 1682.872 kb on - strand at 1682.872 kb on - strand at 1682.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrate 75 mM remove 1,680,002 + Dsui_1599 0.31 -0.6 1,680,010 - Dsui_1599 0.31 -3.2 1,680,102 + Dsui_1599 0.39 -1.1 1,680,251 + Dsui_1599 0.51 -0.6 1,680,366 + Dsui_1599 0.61 -1.6 1,680,389 - Dsui_1599 0.63 -3.0 1,680,394 + Dsui_1599 0.63 -1.1 1,680,584 + Dsui_1599 0.79 -1.6 1,680,592 - Dsui_1599 0.80 -3.8 1,680,769 - -3.3 1,680,849 - -2.4 1,680,851 + +0.4 1,680,859 - -1.7 1,680,861 + -1.9 1,680,868 + -0.6 1,681,078 - Dsui_1600 0.23 -2.3 1,681,090 + Dsui_1600 0.24 -0.6 1,681,113 - Dsui_1600 0.26 -2.4 1,681,131 + +0.4 1,681,189 - Dsui_1600 0.33 -2.7 1,681,386 - Dsui_1600 0.51 -2.3 1,681,471 + Dsui_1600 0.59 +0.4 1,681,504 - Dsui_1600 0.62 -2.7 1,681,504 - Dsui_1600 0.62 -2.7 1,681,513 + +0.4 1,681,518 + -0.6 1,681,656 - Dsui_1600 0.76 -3.1 1,681,783 - Dsui_1600 0.88 -2.8 1,681,890 - -2.6 1,681,919 - -1.3 1,681,930 - -2.4 1,682,738 + -0.6 1,682,762 + +0.4 1,682,774 - -0.6 1,682,779 + -1.1 1,682,846 - -0.4 1,682,859 + -2.1 1,682,865 - -3.5 1,682,869 + +0.4 1,682,872 - -0.6 1,682,872 - -0.3 1,682,872 - -0.4
Or see this region's nucleotide sequence