Experiment: ALP with nitrate 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1159 and Dsui_1160 are separated by 79 nucleotides Dsui_1160 and Dsui_1161 are separated by 2 nucleotides Dsui_1161 and Dsui_1162 are separated by 9 nucleotides
Dsui_1159: Dsui_1159 - hydrogenase expression/formation protein HypD, at 1,215,031 to 1,216,161
_1159
Dsui_1160: Dsui_1160 - hydrogenase assembly chaperone HypC/HupF, at 1,216,241 to 1,216,486
_1160
Dsui_1161: Dsui_1161 - transglutaminase-like enzyme, predicted cysteine protease, at 1,216,489 to 1,217,133
_1161
Dsui_1162: Dsui_1162 - (NiFe) hydrogenase maturation protein HypF, at 1,217,143 to 1,219,482
_1162
Position (kb)
1216
1217
1218 Strain fitness (log2 ratio)
-2
-1
0
1 at 1215.497 kb on - strand, within Dsui_1159 at 1215.550 kb on + strand, within Dsui_1159 at 1215.653 kb on - strand, within Dsui_1159 at 1215.680 kb on + strand at 1215.694 kb on - strand, within Dsui_1159 at 1215.802 kb on + strand at 1215.810 kb on - strand, within Dsui_1159 at 1215.881 kb on - strand, within Dsui_1159 at 1215.900 kb on + strand, within Dsui_1159 at 1215.901 kb on - strand, within Dsui_1159 at 1216.049 kb on - strand at 1216.093 kb on - strand at 1216.240 kb on - strand at 1216.240 kb on - strand at 1216.247 kb on - strand at 1216.268 kb on + strand, within Dsui_1160 at 1216.326 kb on + strand, within Dsui_1160 at 1216.334 kb on - strand, within Dsui_1160 at 1216.337 kb on + strand, within Dsui_1160 at 1216.386 kb on + strand at 1216.390 kb on + strand, within Dsui_1160 at 1216.487 kb on + strand at 1216.495 kb on - strand at 1216.552 kb on + strand at 1216.600 kb on - strand, within Dsui_1161 at 1216.659 kb on - strand, within Dsui_1161 at 1216.659 kb on - strand, within Dsui_1161 at 1216.703 kb on - strand, within Dsui_1161 at 1216.740 kb on - strand, within Dsui_1161 at 1216.786 kb on - strand, within Dsui_1161 at 1216.909 kb on - strand, within Dsui_1161 at 1216.924 kb on + strand at 1217.032 kb on + strand, within Dsui_1161 at 1217.320 kb on - strand at 1217.442 kb on + strand at 1217.459 kb on + strand at 1217.506 kb on - strand, within Dsui_1162 at 1217.516 kb on - strand, within Dsui_1162 at 1217.520 kb on + strand at 1217.529 kb on - strand, within Dsui_1162 at 1217.616 kb on + strand at 1217.624 kb on - strand, within Dsui_1162 at 1217.624 kb on - strand, within Dsui_1162 at 1217.624 kb on - strand, within Dsui_1162 at 1217.633 kb on + strand, within Dsui_1162 at 1217.677 kb on + strand at 1217.677 kb on + strand, within Dsui_1162 at 1217.797 kb on + strand at 1217.968 kb on - strand, within Dsui_1162 at 1218.021 kb on + strand at 1218.048 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrate 75 mM remove 1,215,497 - Dsui_1159 0.41 -0.6 1,215,550 + Dsui_1159 0.46 -0.6 1,215,653 - Dsui_1159 0.55 +0.7 1,215,680 + +0.4 1,215,694 - Dsui_1159 0.59 -2.2 1,215,802 + +0.4 1,215,810 - Dsui_1159 0.69 -1.6 1,215,881 - Dsui_1159 0.75 -0.7 1,215,900 + Dsui_1159 0.77 +0.4 1,215,901 - Dsui_1159 0.77 -0.5 1,216,049 - -1.2 1,216,093 - -0.6 1,216,240 - -0.2 1,216,240 - -0.6 1,216,247 - -0.2 1,216,268 + Dsui_1160 0.11 -1.2 1,216,326 + Dsui_1160 0.35 +0.4 1,216,334 - Dsui_1160 0.38 +0.4 1,216,337 + Dsui_1160 0.39 -1.9 1,216,386 + +0.4 1,216,390 + Dsui_1160 0.61 -0.6 1,216,487 + -0.6 1,216,495 - -1.2 1,216,552 + -0.6 1,216,600 - Dsui_1161 0.17 -1.2 1,216,659 - Dsui_1161 0.26 -0.0 1,216,659 - Dsui_1161 0.26 -1.6 1,216,703 - Dsui_1161 0.33 -0.2 1,216,740 - Dsui_1161 0.39 -0.7 1,216,786 - Dsui_1161 0.46 -1.2 1,216,909 - Dsui_1161 0.65 -1.0 1,216,924 + +0.4 1,217,032 + Dsui_1161 0.84 -0.6 1,217,320 - -0.8 1,217,442 + +0.4 1,217,459 + +0.4 1,217,506 - Dsui_1162 0.16 -1.2 1,217,516 - Dsui_1162 0.16 +0.0 1,217,520 + +0.4 1,217,529 - Dsui_1162 0.16 -1.8 1,217,616 + +0.4 1,217,624 - Dsui_1162 0.21 -2.3 1,217,624 - Dsui_1162 0.21 -0.4 1,217,624 - Dsui_1162 0.21 -0.3 1,217,633 + Dsui_1162 0.21 -0.6 1,217,677 + +0.4 1,217,677 + Dsui_1162 0.23 -1.2 1,217,797 + +0.4 1,217,968 - Dsui_1162 0.35 -0.5 1,218,021 + -1.2 1,218,048 + +0.4
Or see this region's nucleotide sequence