Experiment: ALP with nitrate 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0103 and Dsui_0104 are separated by 107 nucleotides Dsui_0104 and Dsui_0105 are separated by 64 nucleotides Dsui_0105 and Dsui_0106 are separated by 252 nucleotides
Dsui_0103: Dsui_0103 - transcriptional regulator, at 101,774 to 102,364
_0103
Dsui_0104: Dsui_0104 - CBS-domain-containing membrane protein, at 102,472 to 102,966
_0104
Dsui_0105: Dsui_0105 - NAD-dependent aldehyde dehydrogenase, at 103,031 to 104,461
_0105
Dsui_0106: Dsui_0106 - hypothetical protein, at 104,714 to 105,490
_0106
Position (kb) 103
104
105 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 102.071 kb on - strand, within Dsui_0103 at 102.076 kb on - strand, within Dsui_0103 at 102.157 kb on + strand at 102.168 kb on + strand at 102.176 kb on - strand, within Dsui_0103 at 102.186 kb on - strand, within Dsui_0103 at 102.223 kb on + strand at 102.352 kb on - strand at 102.368 kb on - strand at 102.495 kb on + strand at 102.511 kb on + strand at 102.511 kb on + strand at 102.511 kb on + strand at 102.519 kb on - strand at 102.519 kb on - strand at 102.542 kb on + strand at 102.585 kb on - strand at 102.696 kb on + strand, within Dsui_0104 at 102.728 kb on + strand, within Dsui_0104 at 102.748 kb on + strand at 102.842 kb on - strand, within Dsui_0104 at 102.965 kb on - strand at 102.967 kb on - strand at 102.975 kb on - strand at 103.104 kb on - strand at 103.125 kb on - strand at 103.156 kb on - strand at 103.158 kb on + strand at 103.246 kb on - strand, within Dsui_0105 at 103.324 kb on - strand at 103.544 kb on - strand, within Dsui_0105 at 103.548 kb on - strand, within Dsui_0105 at 103.586 kb on - strand at 103.592 kb on - strand at 103.613 kb on + strand at 103.726 kb on + strand at 103.726 kb on + strand, within Dsui_0105 at 103.734 kb on - strand at 103.734 kb on - strand, within Dsui_0105 at 103.734 kb on - strand, within Dsui_0105 at 103.785 kb on + strand, within Dsui_0105 at 103.829 kb on + strand at 103.830 kb on + strand at 103.926 kb on - strand, within Dsui_0105 at 104.029 kb on - strand at 104.042 kb on - strand, within Dsui_0105 at 104.072 kb on + strand, within Dsui_0105 at 104.073 kb on - strand, within Dsui_0105 at 104.149 kb on - strand, within Dsui_0105 at 104.211 kb on + strand at 104.227 kb on + strand at 104.463 kb on + strand at 104.494 kb on + strand at 104.502 kb on - strand at 104.513 kb on + strand at 104.551 kb on - strand at 104.553 kb on + strand at 104.696 kb on + strand at 104.715 kb on - strand at 104.715 kb on - strand at 104.744 kb on - strand at 104.955 kb on - strand, within Dsui_0106 at 104.965 kb on + strand, within Dsui_0106 at 104.984 kb on + strand at 104.997 kb on - strand, within Dsui_0106 at 105.068 kb on + strand, within Dsui_0106 at 105.076 kb on + strand at 105.113 kb on - strand, within Dsui_0106 at 105.202 kb on + strand, within Dsui_0106 at 105.210 kb on - strand at 105.210 kb on - strand, within Dsui_0106 at 105.275 kb on - strand, within Dsui_0106 at 105.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrate 75 mM remove 102,071 - Dsui_0103 0.50 -0.2 102,076 - Dsui_0103 0.51 +0.0 102,157 + -1.0 102,168 + -0.4 102,176 - Dsui_0103 0.68 -0.6 102,186 - Dsui_0103 0.70 -0.4 102,223 + -0.4 102,352 - +0.2 102,368 - +1.2 102,495 + -2.2 102,511 + -0.4 102,511 + +0.6 102,511 + -1.0 102,519 - +0.6 102,519 - -1.4 102,542 + +0.6 102,585 - +0.6 102,696 + Dsui_0104 0.45 -1.0 102,728 + Dsui_0104 0.52 +0.6 102,748 + +0.6 102,842 - Dsui_0104 0.75 +0.6 102,965 - +0.6 102,967 - -1.0 102,975 - -1.0 103,104 - +0.6 103,125 - +0.6 103,156 - -0.4 103,158 + -1.4 103,246 - Dsui_0105 0.15 +0.6 103,324 - -0.4 103,544 - Dsui_0105 0.36 -0.7 103,548 - Dsui_0105 0.36 +0.6 103,586 - +0.6 103,592 - +0.6 103,613 + -1.4 103,726 + +0.6 103,726 + Dsui_0105 0.49 -0.4 103,734 - -0.4 103,734 - Dsui_0105 0.49 -3.1 103,734 - Dsui_0105 0.49 -1.0 103,785 + Dsui_0105 0.53 -3.0 103,829 + +0.6 103,830 + -1.0 103,926 - Dsui_0105 0.63 -3.5 104,029 - +1.2 104,042 - Dsui_0105 0.71 +0.6 104,072 + Dsui_0105 0.73 -1.7 104,073 - Dsui_0105 0.73 -0.4 104,149 - Dsui_0105 0.78 -3.1 104,211 + +0.6 104,227 + -1.7 104,463 + -0.4 104,494 + +0.6 104,502 - -0.4 104,513 + +0.6 104,551 - +0.6 104,553 + -0.4 104,696 + +0.6 104,715 - +0.3 104,715 - -2.4 104,744 - -0.2 104,955 - Dsui_0106 0.31 +0.6 104,965 + Dsui_0106 0.32 -1.0 104,984 + -0.4 104,997 - Dsui_0106 0.36 -2.5 105,068 + Dsui_0106 0.46 -0.4 105,076 + +0.6 105,113 - Dsui_0106 0.51 +1.6 105,202 + Dsui_0106 0.63 -1.0 105,210 - +0.6 105,210 - Dsui_0106 0.64 -0.4 105,275 - Dsui_0106 0.72 -0.4 105,458 - -0.1
Or see this region's nucleotide sequence