Experiment: ALP with nitrate 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0011 and Dsui_0012 are separated by 221 nucleotides Dsui_0012 and Dsui_0013 are separated by 1 nucleotides Dsui_0013 and Dsui_0014 are separated by 496 nucleotides
Dsui_0011: Dsui_0011 - isocitrate lyase, at 9,345 to 10,655
_0011
Dsui_0012: Dsui_0012 - putative permease, DMT superfamily, at 10,877 to 11,764
_0012
Dsui_0013: Dsui_0013 - Thioredoxin, at 11,766 to 12,266
_0013
Dsui_0014: Dsui_0014 - putative membrane protein, at 12,763 to 13,377
_0014
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 9.928 kb on + strand, within Dsui_0011 at 9.965 kb on - strand, within Dsui_0011 at 10.018 kb on - strand, within Dsui_0011 at 10.018 kb on - strand, within Dsui_0011 at 10.035 kb on - strand, within Dsui_0011 at 10.040 kb on - strand, within Dsui_0011 at 10.057 kb on + strand, within Dsui_0011 at 10.065 kb on - strand, within Dsui_0011 at 10.150 kb on + strand, within Dsui_0011 at 10.158 kb on - strand, within Dsui_0011 at 10.258 kb on - strand, within Dsui_0011 at 10.258 kb on - strand, within Dsui_0011 at 10.297 kb on - strand, within Dsui_0011 at 10.371 kb on + strand, within Dsui_0011 at 10.432 kb on + strand, within Dsui_0011 at 10.456 kb on - strand, within Dsui_0011 at 10.456 kb on - strand, within Dsui_0011 at 10.467 kb on + strand, within Dsui_0011 at 10.531 kb on - strand at 10.614 kb on - strand at 10.625 kb on - strand at 10.625 kb on - strand at 10.690 kb on - strand at 10.748 kb on - strand at 10.773 kb on + strand at 10.773 kb on - strand at 10.781 kb on - strand at 10.781 kb on - strand at 10.781 kb on - strand at 10.781 kb on - strand at 10.781 kb on - strand at 10.781 kb on - strand at 10.833 kb on - strand at 10.854 kb on + strand at 10.867 kb on + strand at 10.867 kb on + strand at 10.875 kb on - strand at 11.015 kb on - strand, within Dsui_0012 at 11.021 kb on - strand at 11.045 kb on + strand, within Dsui_0012 at 11.105 kb on - strand, within Dsui_0012 at 11.110 kb on - strand, within Dsui_0012 at 11.163 kb on - strand, within Dsui_0012 at 11.255 kb on - strand, within Dsui_0012 at 11.267 kb on - strand, within Dsui_0012 at 11.274 kb on - strand, within Dsui_0012 at 11.343 kb on + strand, within Dsui_0012 at 11.408 kb on + strand, within Dsui_0012 at 11.428 kb on - strand, within Dsui_0012 at 11.434 kb on + strand, within Dsui_0012 at 11.471 kb on + strand, within Dsui_0012 at 11.524 kb on - strand at 11.536 kb on + strand, within Dsui_0012 at 11.536 kb on + strand, within Dsui_0012 at 11.547 kb on + strand, within Dsui_0012 at 11.550 kb on - strand, within Dsui_0012 at 11.598 kb on + strand, within Dsui_0012 at 11.610 kb on - strand at 11.610 kb on - strand, within Dsui_0012 at 11.666 kb on - strand, within Dsui_0012 at 11.676 kb on + strand at 11.714 kb on + strand at 11.727 kb on - strand at 11.759 kb on + strand at 11.767 kb on - strand at 11.772 kb on - strand at 11.951 kb on + strand at 11.983 kb on - strand, within Dsui_0013 at 12.306 kb on + strand at 12.374 kb on + strand at 12.604 kb on - strand at 12.609 kb on - strand at 12.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrate 75 mM remove 9,928 + Dsui_0011 0.44 -0.4 9,965 - Dsui_0011 0.47 +0.6 10,018 - Dsui_0011 0.51 -1.4 10,018 - Dsui_0011 0.51 -1.0 10,035 - Dsui_0011 0.53 +0.6 10,040 - Dsui_0011 0.53 -0.4 10,057 + Dsui_0011 0.54 -0.4 10,065 - Dsui_0011 0.55 +0.6 10,150 + Dsui_0011 0.61 -0.4 10,158 - Dsui_0011 0.62 -1.7 10,258 - Dsui_0011 0.70 -1.0 10,258 - Dsui_0011 0.70 +0.6 10,297 - Dsui_0011 0.73 -1.0 10,371 + Dsui_0011 0.78 -3.4 10,432 + Dsui_0011 0.83 -2.5 10,456 - Dsui_0011 0.85 +0.6 10,456 - Dsui_0011 0.85 +0.6 10,467 + Dsui_0011 0.86 -2.7 10,531 - -2.7 10,614 - +0.6 10,625 - -1.4 10,625 - +0.6 10,690 - -0.4 10,748 - +0.6 10,773 + +1.4 10,773 - +0.6 10,781 - -0.4 10,781 - +0.6 10,781 - +0.6 10,781 - +0.6 10,781 - +0.6 10,781 - +0.6 10,833 - -1.0 10,854 + +0.0 10,867 + -0.4 10,867 + -1.5 10,875 - +0.6 11,015 - Dsui_0012 0.16 -1.0 11,021 - +0.6 11,045 + Dsui_0012 0.19 -1.2 11,105 - Dsui_0012 0.26 -1.7 11,110 - Dsui_0012 0.26 -1.4 11,163 - Dsui_0012 0.32 -2.5 11,255 - Dsui_0012 0.43 -1.0 11,267 - Dsui_0012 0.44 +0.6 11,274 - Dsui_0012 0.45 +0.6 11,343 + Dsui_0012 0.52 +0.3 11,408 + Dsui_0012 0.60 -0.2 11,428 - Dsui_0012 0.62 -2.2 11,434 + Dsui_0012 0.63 -0.9 11,471 + Dsui_0012 0.67 -0.8 11,524 - -0.4 11,536 + Dsui_0012 0.74 -3.0 11,536 + Dsui_0012 0.74 -1.8 11,547 + Dsui_0012 0.75 +0.2 11,550 - Dsui_0012 0.76 +0.6 11,598 + Dsui_0012 0.81 -1.2 11,610 - -0.4 11,610 - Dsui_0012 0.83 -0.4 11,666 - Dsui_0012 0.89 -1.0 11,676 + -0.0 11,714 + -0.7 11,727 - -2.7 11,759 + -0.4 11,767 - +1.0 11,772 - -0.1 11,951 + +0.6 11,983 - Dsui_0013 0.43 +0.2 12,306 + +0.6 12,374 + +0.6 12,604 - +0.6 12,609 - -2.2 12,711 - -0.4
Or see this region's nucleotide sequence