Experiment: ALP with nitrate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0026 and Dsui_0027 are separated by 214 nucleotides Dsui_0027 and Dsui_0028 overlap by 4 nucleotides Dsui_0028 and Dsui_0029 are separated by 83 nucleotides Dsui_0029 and Dsui_0030 are separated by 141 nucleotides
Dsui_0026: Dsui_0026 - CBS domain-containing protein, at 23,840 to 25,126
_0026
Dsui_0027: Dsui_0027 - putative effector of murein hydrolase LrgA, at 25,341 to 25,703
_0027
Dsui_0028: Dsui_0028 - putative effector of murein hydrolase, at 25,700 to 26,455
_0028
Dsui_0029: Dsui_0029 - putative pyrophosphatase, at 26,539 to 26,886
_0029
Dsui_0030: Dsui_0030 - FtsH-interacting integral membrane protein, at 27,028 to 27,720
_0030
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 24.706 kb on + strand, within Dsui_0026 at 24.741 kb on + strand at 24.749 kb on - strand, within Dsui_0026 at 24.817 kb on + strand at 24.823 kb on + strand, within Dsui_0026 at 24.831 kb on - strand, within Dsui_0026 at 24.853 kb on + strand at 24.861 kb on - strand, within Dsui_0026 at 24.918 kb on - strand, within Dsui_0026 at 24.976 kb on - strand, within Dsui_0026 at 25.099 kb on - strand at 25.190 kb on - strand at 25.233 kb on - strand at 25.287 kb on - strand at 25.355 kb on + strand at 25.396 kb on + strand at 25.480 kb on + strand at 25.498 kb on - strand at 25.596 kb on + strand, within Dsui_0027 at 25.660 kb on - strand, within Dsui_0027 at 25.667 kb on - strand at 25.667 kb on - strand at 25.739 kb on + strand at 25.741 kb on - strand at 25.777 kb on - strand, within Dsui_0028 at 25.785 kb on + strand, within Dsui_0028 at 25.793 kb on - strand at 25.828 kb on - strand at 25.979 kb on + strand, within Dsui_0028 at 26.007 kb on - strand, within Dsui_0028 at 26.008 kb on + strand, within Dsui_0028 at 26.008 kb on + strand, within Dsui_0028 at 26.016 kb on - strand, within Dsui_0028 at 26.028 kb on - strand, within Dsui_0028 at 26.028 kb on - strand, within Dsui_0028 at 26.044 kb on + strand at 26.235 kb on - strand, within Dsui_0028 at 26.262 kb on + strand, within Dsui_0028 at 26.275 kb on + strand at 26.345 kb on - strand, within Dsui_0028 at 26.377 kb on + strand, within Dsui_0028 at 26.386 kb on + strand at 26.434 kb on + strand at 26.538 kb on + strand at 26.545 kb on - strand at 26.567 kb on - strand at 26.574 kb on - strand, within Dsui_0029 at 26.665 kb on - strand, within Dsui_0029 at 26.671 kb on + strand, within Dsui_0029 at 26.724 kb on + strand, within Dsui_0029 at 26.732 kb on - strand, within Dsui_0029 at 26.785 kb on - strand at 26.810 kb on + strand, within Dsui_0029 at 26.825 kb on + strand, within Dsui_0029 at 26.825 kb on + strand, within Dsui_0029 at 27.046 kb on + strand at 27.054 kb on - strand at 27.079 kb on + strand at 27.085 kb on + strand at 27.087 kb on - strand at 27.087 kb on - strand at 27.110 kb on + strand, within Dsui_0030 at 27.150 kb on + strand, within Dsui_0030 at 27.306 kb on + strand at 27.343 kb on + strand at 27.385 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrate 50 mM remove 24,706 + Dsui_0026 0.67 -0.5 24,741 + +0.5 24,749 - Dsui_0026 0.71 -1.1 24,817 + +0.5 24,823 + Dsui_0026 0.76 -1.3 24,831 - Dsui_0026 0.77 -0.5 24,853 + +0.5 24,861 - Dsui_0026 0.79 +0.5 24,918 - Dsui_0026 0.84 -2.3 24,976 - Dsui_0026 0.88 +0.5 25,099 - +0.5 25,190 - +0.5 25,233 - -0.5 25,287 - -0.5 25,355 + -0.5 25,396 + +0.5 25,480 + +0.5 25,498 - -1.1 25,596 + Dsui_0027 0.70 +0.5 25,660 - Dsui_0027 0.88 +0.5 25,667 - +0.5 25,667 - -0.5 25,739 + +0.5 25,741 - -1.1 25,777 - Dsui_0028 0.10 -1.8 25,785 + Dsui_0028 0.11 -0.5 25,793 - +0.5 25,828 - +0.5 25,979 + Dsui_0028 0.37 +0.5 26,007 - Dsui_0028 0.41 +0.5 26,008 + Dsui_0028 0.41 -0.5 26,008 + Dsui_0028 0.41 -2.1 26,016 - Dsui_0028 0.42 -1.1 26,028 - Dsui_0028 0.43 +0.5 26,028 - Dsui_0028 0.43 -1.1 26,044 + -0.5 26,235 - Dsui_0028 0.71 -1.1 26,262 + Dsui_0028 0.74 -1.1 26,275 + +0.5 26,345 - Dsui_0028 0.85 +0.5 26,377 + Dsui_0028 0.90 -2.1 26,386 + +0.5 26,434 + -1.1 26,538 + +0.5 26,545 - -0.5 26,567 - -0.5 26,574 - Dsui_0029 0.10 -0.5 26,665 - Dsui_0029 0.36 +0.5 26,671 + Dsui_0029 0.38 -0.5 26,724 + Dsui_0029 0.53 -1.5 26,732 - Dsui_0029 0.55 -0.5 26,785 - +0.5 26,810 + Dsui_0029 0.78 +0.5 26,825 + Dsui_0029 0.82 -1.1 26,825 + Dsui_0029 0.82 -0.5 27,046 + +0.5 27,054 - +0.5 27,079 + +0.5 27,085 + +0.5 27,087 - +0.5 27,087 - -1.8 27,110 + Dsui_0030 0.12 -1.5 27,150 + Dsui_0030 0.18 -2.5 27,306 + +0.5 27,343 + -1.1 27,385 + +0.5
Or see this region's nucleotide sequence