Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_19340 and AO353_19345 are separated by 110 nucleotides AO353_19345 and AO353_19350 are separated by 287 nucleotides AO353_19350 and AO353_19355 are separated by 75 nucleotides AO353_19355 and AO353_19360 overlap by 25 nucleotides
AO353_19340: AO353_19340 - amino acid ABC transporter substrate-binding protein, at 4,239,291 to 4,240,088
_19340
AO353_19345: AO353_19345 - RNA polymerase subunit sigma-70, at 4,240,199 to 4,240,747
_19345
AO353_19350: AO353_19350 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 4,241,035 to 4,241,847
_19350
AO353_19355: AO353_19355 - dihydroneopterin aldolase, at 4,241,923 to 4,242,690
_19355
AO353_19360: AO353_19360 - peroxiredoxin, at 4,242,666 to 4,243,532
_19360
Position (kb) 4241
4242 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4240.053 kb on + strand at 4240.053 kb on + strand at 4240.053 kb on + strand at 4240.054 kb on - strand at 4240.054 kb on - strand at 4240.054 kb on - strand at 4240.054 kb on - strand at 4240.054 kb on - strand at 4240.054 kb on - strand at 4240.079 kb on + strand at 4240.079 kb on + strand at 4240.079 kb on + strand at 4240.079 kb on + strand at 4240.079 kb on + strand at 4240.079 kb on + strand at 4240.146 kb on - strand at 4240.216 kb on + strand at 4240.217 kb on - strand at 4240.217 kb on - strand at 4240.341 kb on + strand, within AO353_19345 at 4240.341 kb on + strand, within AO353_19345 at 4240.341 kb on + strand, within AO353_19345 at 4240.342 kb on - strand, within AO353_19345 at 4240.342 kb on - strand, within AO353_19345 at 4240.342 kb on - strand, within AO353_19345 at 4240.373 kb on + strand, within AO353_19345 at 4240.485 kb on + strand, within AO353_19345 at 4240.486 kb on - strand, within AO353_19345 at 4240.551 kb on + strand, within AO353_19345 at 4240.551 kb on + strand, within AO353_19345 at 4240.551 kb on + strand, within AO353_19345 at 4240.551 kb on + strand, within AO353_19345 at 4240.551 kb on + strand, within AO353_19345 at 4240.551 kb on + strand, within AO353_19345 at 4240.552 kb on - strand, within AO353_19345 at 4240.552 kb on - strand, within AO353_19345 at 4240.552 kb on - strand, within AO353_19345 at 4240.687 kb on + strand, within AO353_19345 at 4240.688 kb on - strand, within AO353_19345 at 4240.688 kb on - strand, within AO353_19345 at 4240.688 kb on - strand, within AO353_19345 at 4240.706 kb on + strand at 4240.706 kb on + strand at 4240.707 kb on - strand at 4240.771 kb on + strand at 4240.772 kb on - strand at 4240.775 kb on + strand at 4240.775 kb on + strand at 4240.775 kb on + strand at 4240.775 kb on + strand at 4240.775 kb on + strand at 4240.775 kb on + strand at 4240.776 kb on - strand at 4240.776 kb on - strand at 4240.776 kb on - strand at 4240.776 kb on - strand at 4240.776 kb on - strand at 4240.805 kb on + strand at 4240.805 kb on + strand at 4240.806 kb on - strand at 4240.806 kb on - strand at 4240.921 kb on - strand at 4240.921 kb on - strand at 4240.941 kb on + strand at 4240.941 kb on + strand at 4240.950 kb on + strand at 4240.950 kb on + strand at 4240.950 kb on + strand at 4241.071 kb on + strand at 4241.071 kb on + strand at 4241.071 kb on + strand at 4241.071 kb on + strand at 4241.071 kb on + strand at 4241.071 kb on + strand at 4241.071 kb on + strand at 4241.072 kb on - strand at 4241.072 kb on - strand at 4241.072 kb on - strand at 4241.072 kb on - strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.116 kb on + strand at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.117 kb on - strand, within AO353_19350 at 4241.261 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.392 kb on + strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.393 kb on - strand, within AO353_19350 at 4241.426 kb on - strand, within AO353_19350 at 4241.426 kb on - strand, within AO353_19350 at 4241.426 kb on - strand, within AO353_19350 at 4241.426 kb on - strand, within AO353_19350 at 4241.428 kb on + strand, within AO353_19350 at 4241.428 kb on + strand, within AO353_19350 at 4241.428 kb on + strand, within AO353_19350 at 4241.429 kb on - strand, within AO353_19350 at 4241.429 kb on - strand, within AO353_19350 at 4241.429 kb on - strand, within AO353_19350 at 4241.429 kb on - strand, within AO353_19350 at 4241.429 kb on - strand, within AO353_19350 at 4241.429 kb on - strand, within AO353_19350 at 4241.429 kb on - strand, within AO353_19350 at 4241.480 kb on + strand, within AO353_19350 at 4241.480 kb on + strand, within AO353_19350 at 4241.480 kb on + strand, within AO353_19350 at 4241.539 kb on + strand, within AO353_19350 at 4241.540 kb on - strand, within AO353_19350 at 4241.616 kb on + strand, within AO353_19350 at 4241.616 kb on + strand, within AO353_19350 at 4241.616 kb on + strand, within AO353_19350 at 4241.616 kb on + strand, within AO353_19350 at 4241.616 kb on + strand, within AO353_19350 at 4241.617 kb on - strand, within AO353_19350 at 4241.617 kb on - strand, within AO353_19350 at 4241.617 kb on - strand, within AO353_19350 at 4241.617 kb on - strand, within AO353_19350 at 4241.752 kb on + strand, within AO353_19350 at 4241.845 kb on + strand at 4241.845 kb on + strand at 4241.846 kb on - strand at 4241.846 kb on - strand at 4241.846 kb on - strand at 4241.902 kb on + strand at 4241.902 kb on + strand at 4241.903 kb on - strand at 4241.925 kb on - strand at 4241.948 kb on - strand at 4241.948 kb on - strand at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.054 kb on + strand, within AO353_19355 at 4242.054 kb on + strand, within AO353_19355 at 4242.055 kb on - strand, within AO353_19355 at 4242.055 kb on - strand, within AO353_19355 at 4242.055 kb on - strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.314 kb on + strand, within AO353_19355 at 4242.314 kb on + strand, within AO353_19355 at 4242.315 kb on - strand, within AO353_19355 at 4242.315 kb on - strand, within AO353_19355 at 4242.315 kb on - strand, within AO353_19355 at 4242.401 kb on + strand, within AO353_19355 at 4242.401 kb on + strand, within AO353_19355 at 4242.453 kb on - strand, within AO353_19355 at 4242.494 kb on + strand, within AO353_19355 at 4242.495 kb on - strand, within AO353_19355 at 4242.495 kb on - strand, within AO353_19355 at 4242.495 kb on - strand, within AO353_19355 at 4242.518 kb on + strand, within AO353_19355 at 4242.519 kb on - strand, within AO353_19355 at 4242.817 kb on + strand, within AO353_19360 at 4242.827 kb on + strand, within AO353_19360
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 4,240,053 + +2.1 4,240,053 + +0.7 4,240,053 + +1.2 4,240,054 - +1.2 4,240,054 - +0.4 4,240,054 - +1.5 4,240,054 - +1.4 4,240,054 - +0.0 4,240,054 - +2.3 4,240,079 + +0.6 4,240,079 + +1.9 4,240,079 + +0.6 4,240,079 + +1.1 4,240,079 + +1.2 4,240,079 + +0.6 4,240,146 - +0.9 4,240,216 + -0.3 4,240,217 - +0.2 4,240,217 - +0.1 4,240,341 + AO353_19345 0.26 -0.8 4,240,341 + AO353_19345 0.26 -0.1 4,240,341 + AO353_19345 0.26 -0.8 4,240,342 - AO353_19345 0.26 -0.0 4,240,342 - AO353_19345 0.26 +2.2 4,240,342 - AO353_19345 0.26 +0.8 4,240,373 + AO353_19345 0.32 +0.8 4,240,485 + AO353_19345 0.52 -2.3 4,240,486 - AO353_19345 0.52 +2.3 4,240,551 + AO353_19345 0.64 -0.8 4,240,551 + AO353_19345 0.64 -0.3 4,240,551 + AO353_19345 0.64 +1.9 4,240,551 + AO353_19345 0.64 -2.1 4,240,551 + AO353_19345 0.64 -0.6 4,240,551 + AO353_19345 0.64 -0.3 4,240,552 - AO353_19345 0.64 +0.4 4,240,552 - AO353_19345 0.64 +1.4 4,240,552 - AO353_19345 0.64 +0.6 4,240,687 + AO353_19345 0.89 -0.4 4,240,688 - AO353_19345 0.89 +0.4 4,240,688 - AO353_19345 0.89 +0.1 4,240,688 - AO353_19345 0.89 +0.9 4,240,706 + +2.4 4,240,706 + -0.4 4,240,707 - +1.4 4,240,771 + -0.7 4,240,772 - -0.7 4,240,775 + +0.2 4,240,775 + -0.7 4,240,775 + +0.1 4,240,775 + +0.0 4,240,775 + +0.3 4,240,775 + -0.4 4,240,776 - +0.1 4,240,776 - +0.5 4,240,776 - -2.1 4,240,776 - -0.1 4,240,776 - -2.8 4,240,805 + +0.4 4,240,805 + +1.9 4,240,806 - -1.6 4,240,806 - -0.8 4,240,921 - -1.4 4,240,921 - -0.6 4,240,941 + -0.1 4,240,941 + -0.9 4,240,950 + +0.6 4,240,950 + -1.4 4,240,950 + +0.7 4,241,071 + +0.4 4,241,071 + -0.8 4,241,071 + -0.4 4,241,071 + -1.4 4,241,071 + +1.3 4,241,071 + -0.4 4,241,071 + -1.1 4,241,072 - -2.7 4,241,072 - -0.1 4,241,072 - -0.9 4,241,072 - -1.7 4,241,116 + +1.5 4,241,116 + +0.3 4,241,116 + +1.1 4,241,116 + +1.0 4,241,116 + -0.4 4,241,116 + +0.5 4,241,116 + -0.9 4,241,116 + +0.6 4,241,116 + -0.1 4,241,116 + -0.2 4,241,116 + -1.2 4,241,116 + -0.0 4,241,117 - AO353_19350 0.10 +0.1 4,241,117 - AO353_19350 0.10 +0.3 4,241,117 - AO353_19350 0.10 -1.5 4,241,117 - AO353_19350 0.10 -0.7 4,241,117 - AO353_19350 0.10 +1.1 4,241,117 - AO353_19350 0.10 -0.1 4,241,117 - AO353_19350 0.10 -1.2 4,241,117 - AO353_19350 0.10 +1.0 4,241,117 - AO353_19350 0.10 -1.2 4,241,117 - AO353_19350 0.10 -0.7 4,241,117 - AO353_19350 0.10 -0.1 4,241,117 - AO353_19350 0.10 +0.3 4,241,117 - AO353_19350 0.10 -0.5 4,241,261 + AO353_19350 0.28 -0.4 4,241,392 + AO353_19350 0.44 -1.4 4,241,392 + AO353_19350 0.44 +0.0 4,241,392 + AO353_19350 0.44 -0.6 4,241,392 + AO353_19350 0.44 -0.5 4,241,392 + AO353_19350 0.44 -0.4 4,241,392 + AO353_19350 0.44 -0.4 4,241,392 + AO353_19350 0.44 -0.2 4,241,392 + AO353_19350 0.44 +0.0 4,241,392 + AO353_19350 0.44 +1.2 4,241,392 + AO353_19350 0.44 -0.9 4,241,392 + AO353_19350 0.44 -0.0 4,241,392 + AO353_19350 0.44 -0.3 4,241,392 + AO353_19350 0.44 +0.1 4,241,392 + AO353_19350 0.44 +0.2 4,241,392 + AO353_19350 0.44 +0.5 4,241,392 + AO353_19350 0.44 -0.5 4,241,392 + AO353_19350 0.44 -1.1 4,241,392 + AO353_19350 0.44 -1.4 4,241,392 + -1.2 4,241,392 + AO353_19350 0.44 -0.5 4,241,392 + AO353_19350 0.44 +0.5 4,241,392 + AO353_19350 0.44 +1.0 4,241,393 - AO353_19350 0.44 +0.2 4,241,393 - AO353_19350 0.44 -1.1 4,241,393 - AO353_19350 0.44 +0.3 4,241,393 - AO353_19350 0.44 +0.3 4,241,393 - AO353_19350 0.44 +0.6 4,241,393 - AO353_19350 0.44 -1.0 4,241,393 - AO353_19350 0.44 -1.0 4,241,393 - AO353_19350 0.44 -0.1 4,241,393 - AO353_19350 0.44 +0.6 4,241,393 - AO353_19350 0.44 -0.5 4,241,393 - AO353_19350 0.44 +0.4 4,241,393 - AO353_19350 0.44 -0.6 4,241,393 - AO353_19350 0.44 -2.1 4,241,393 - AO353_19350 0.44 +0.2 4,241,393 - AO353_19350 0.44 -0.9 4,241,426 - AO353_19350 0.48 -1.2 4,241,426 - AO353_19350 0.48 -2.7 4,241,426 - AO353_19350 0.48 -1.2 4,241,426 - AO353_19350 0.48 -0.4 4,241,428 + AO353_19350 0.48 +0.8 4,241,428 + AO353_19350 0.48 -1.0 4,241,428 + AO353_19350 0.48 -0.1 4,241,429 - AO353_19350 0.48 +0.8 4,241,429 - AO353_19350 0.48 +0.3 4,241,429 - AO353_19350 0.48 -0.3 4,241,429 - AO353_19350 0.48 +0.5 4,241,429 - AO353_19350 0.48 +0.2 4,241,429 - AO353_19350 0.48 +0.1 4,241,429 - AO353_19350 0.48 -2.1 4,241,480 + AO353_19350 0.55 -0.6 4,241,480 + AO353_19350 0.55 +0.1 4,241,480 + AO353_19350 0.55 -1.7 4,241,539 + AO353_19350 0.62 -0.3 4,241,540 - AO353_19350 0.62 +0.4 4,241,616 + AO353_19350 0.71 -0.5 4,241,616 + AO353_19350 0.71 -0.4 4,241,616 + AO353_19350 0.71 -1.2 4,241,616 + AO353_19350 0.71 +0.2 4,241,616 + AO353_19350 0.71 +1.3 4,241,617 - AO353_19350 0.72 +0.1 4,241,617 - AO353_19350 0.72 -1.6 4,241,617 - AO353_19350 0.72 +0.8 4,241,617 - AO353_19350 0.72 -0.7 4,241,752 + AO353_19350 0.88 +0.1 4,241,845 + +0.5 4,241,845 + -0.9 4,241,846 - +0.3 4,241,846 - -0.3 4,241,846 - -0.1 4,241,902 + +1.1 4,241,902 + -1.0 4,241,903 - -0.0 4,241,925 - +0.7 4,241,948 - +1.0 4,241,948 - -1.0 4,242,005 + AO353_19355 0.11 +0.4 4,242,005 + AO353_19355 0.11 -0.1 4,242,005 + AO353_19355 0.11 +1.4 4,242,005 + AO353_19355 0.11 -0.7 4,242,005 + AO353_19355 0.11 +0.1 4,242,005 + AO353_19355 0.11 +1.0 4,242,005 + AO353_19355 0.11 +0.0 4,242,005 + AO353_19355 0.11 +1.1 4,242,005 + AO353_19355 0.11 +0.4 4,242,005 + AO353_19355 0.11 +1.3 4,242,005 + AO353_19355 0.11 +0.8 4,242,006 - AO353_19355 0.11 +0.5 4,242,006 - AO353_19355 0.11 +0.2 4,242,006 - AO353_19355 0.11 +0.5 4,242,006 - AO353_19355 0.11 -1.0 4,242,006 - AO353_19355 0.11 +1.9 4,242,006 - AO353_19355 0.11 +1.0 4,242,006 - AO353_19355 0.11 -0.0 4,242,006 - AO353_19355 0.11 +0.4 4,242,006 - AO353_19355 0.11 -1.3 4,242,054 + AO353_19355 0.17 -0.4 4,242,054 + AO353_19355 0.17 -0.2 4,242,055 - AO353_19355 0.17 -0.5 4,242,055 - AO353_19355 0.17 +2.2 4,242,055 - AO353_19355 0.17 -0.7 4,242,279 + AO353_19355 0.46 -0.9 4,242,279 + AO353_19355 0.46 +1.9 4,242,279 + AO353_19355 0.46 +1.3 4,242,279 + AO353_19355 0.46 -0.5 4,242,279 + AO353_19355 0.46 -0.9 4,242,279 + AO353_19355 0.46 -0.2 4,242,279 + AO353_19355 0.46 +0.1 4,242,280 - AO353_19355 0.46 -0.1 4,242,280 - AO353_19355 0.46 -0.5 4,242,280 - AO353_19355 0.46 -0.6 4,242,280 - AO353_19355 0.46 +0.1 4,242,280 - AO353_19355 0.46 -0.8 4,242,280 - AO353_19355 0.46 +1.0 4,242,280 - AO353_19355 0.46 -1.0 4,242,280 - AO353_19355 0.46 -1.5 4,242,280 - AO353_19355 0.46 +0.6 4,242,280 - AO353_19355 0.46 -1.2 4,242,280 - AO353_19355 0.46 +0.3 4,242,280 - AO353_19355 0.46 +0.5 4,242,280 - AO353_19355 0.46 -0.4 4,242,280 - AO353_19355 0.46 -0.3 4,242,280 - AO353_19355 0.46 -0.1 4,242,280 - AO353_19355 0.46 -1.4 4,242,314 + AO353_19355 0.51 -0.7 4,242,314 + AO353_19355 0.51 +0.4 4,242,315 - AO353_19355 0.51 -1.7 4,242,315 - AO353_19355 0.51 -0.1 4,242,315 - AO353_19355 0.51 -0.1 4,242,401 + AO353_19355 0.62 +0.6 4,242,401 + AO353_19355 0.62 +0.6 4,242,453 - AO353_19355 0.69 -1.1 4,242,494 + AO353_19355 0.74 +0.2 4,242,495 - AO353_19355 0.74 +0.6 4,242,495 - AO353_19355 0.74 +0.1 4,242,495 - AO353_19355 0.74 -0.6 4,242,518 + AO353_19355 0.77 -0.4 4,242,519 - AO353_19355 0.78 -0.1 4,242,817 + AO353_19360 0.17 -0.4 4,242,827 + AO353_19360 0.19 -1.1
Or see this region's nucleotide sequence