Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4689 and Pf1N1B4_4690 are separated by 221 nucleotides Pf1N1B4_4690 and Pf1N1B4_4691 overlap by 1 nucleotides Pf1N1B4_4691 and Pf1N1B4_4692 are separated by 454 nucleotides
Pf1N1B4_4689 - hydrolase, alpha/beta fold family, at 5,181,501 to 5,182,544
_4689
Pf1N1B4_4690 - probable membrane protein YPO3302, at 5,182,766 to 5,183,200
_4690
Pf1N1B4_4691 - CopG protein, at 5,183,200 to 5,183,646
_4691
Pf1N1B4_4692 - hypothetical protein, at 5,184,101 to 5,184,631
_4692
Position (kb) 5182
5183
5184 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5181.766 kb on + strand, within Pf1N1B4_4689 at 5181.767 kb on - strand, within Pf1N1B4_4689 at 5181.854 kb on - strand at 5181.898 kb on + strand, within Pf1N1B4_4689 at 5181.899 kb on - strand, within Pf1N1B4_4689 at 5181.899 kb on - strand, within Pf1N1B4_4689 at 5181.899 kb on - strand, within Pf1N1B4_4689 at 5181.901 kb on + strand, within Pf1N1B4_4689 at 5181.901 kb on + strand at 5181.901 kb on + strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.958 kb on + strand, within Pf1N1B4_4689 at 5181.980 kb on - strand, within Pf1N1B4_4689 at 5182.055 kb on - strand, within Pf1N1B4_4689 at 5182.174 kb on + strand, within Pf1N1B4_4689 at 5182.354 kb on + strand, within Pf1N1B4_4689 at 5182.396 kb on + strand, within Pf1N1B4_4689 at 5182.397 kb on - strand, within Pf1N1B4_4689 at 5182.397 kb on - strand at 5182.397 kb on - strand, within Pf1N1B4_4689 at 5182.408 kb on + strand, within Pf1N1B4_4689 at 5182.601 kb on + strand at 5182.607 kb on + strand at 5182.607 kb on + strand at 5182.607 kb on + strand at 5182.608 kb on - strand at 5182.786 kb on + strand at 5182.917 kb on - strand, within Pf1N1B4_4690 at 5183.188 kb on - strand at 5183.264 kb on + strand, within Pf1N1B4_4691 at 5183.362 kb on + strand, within Pf1N1B4_4691 at 5183.362 kb on + strand, within Pf1N1B4_4691 at 5183.363 kb on - strand, within Pf1N1B4_4691 at 5183.406 kb on + strand, within Pf1N1B4_4691 at 5183.483 kb on + strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.696 kb on - strand at 5183.728 kb on + strand at 5183.728 kb on + strand at 5183.837 kb on + strand at 5183.921 kb on + strand at 5183.961 kb on - strand at 5184.005 kb on + strand at 5184.005 kb on + strand at 5184.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 5,181,766 + Pf1N1B4_4689 0.25 +1.5 5,181,767 - Pf1N1B4_4689 0.25 +0.4 5,181,854 - +2.1 5,181,898 + Pf1N1B4_4689 0.38 -1.3 5,181,899 - Pf1N1B4_4689 0.38 -0.8 5,181,899 - Pf1N1B4_4689 0.38 +0.2 5,181,899 - Pf1N1B4_4689 0.38 -2.4 5,181,901 + Pf1N1B4_4689 0.38 -0.6 5,181,901 + +0.6 5,181,901 + Pf1N1B4_4689 0.38 +0.1 5,181,902 - Pf1N1B4_4689 0.38 +0.1 5,181,902 - Pf1N1B4_4689 0.38 -1.0 5,181,902 - +1.0 5,181,902 - Pf1N1B4_4689 0.38 -1.3 5,181,902 - Pf1N1B4_4689 0.38 +0.4 5,181,902 - Pf1N1B4_4689 0.38 +1.1 5,181,902 - Pf1N1B4_4689 0.38 +0.4 5,181,958 + Pf1N1B4_4689 0.44 -0.6 5,181,980 - Pf1N1B4_4689 0.46 +0.6 5,182,055 - Pf1N1B4_4689 0.53 +0.6 5,182,174 + Pf1N1B4_4689 0.64 +0.5 5,182,354 + Pf1N1B4_4689 0.82 -0.6 5,182,396 + Pf1N1B4_4689 0.86 -2.6 5,182,397 - Pf1N1B4_4689 0.86 +0.6 5,182,397 - +0.6 5,182,397 - Pf1N1B4_4689 0.86 +1.0 5,182,408 + Pf1N1B4_4689 0.87 +1.2 5,182,601 + -1.3 5,182,607 + +1.1 5,182,607 + -0.4 5,182,607 + -0.3 5,182,608 - +0.1 5,182,786 + -0.3 5,182,917 - Pf1N1B4_4690 0.35 +0.4 5,183,188 - -1.3 5,183,264 + Pf1N1B4_4691 0.14 -1.0 5,183,362 + Pf1N1B4_4691 0.36 -0.3 5,183,362 + Pf1N1B4_4691 0.36 +0.1 5,183,363 - Pf1N1B4_4691 0.36 +0.6 5,183,406 + Pf1N1B4_4691 0.46 -0.6 5,183,483 + Pf1N1B4_4691 0.63 +1.6 5,183,484 - Pf1N1B4_4691 0.64 +0.1 5,183,484 - Pf1N1B4_4691 0.64 +0.2 5,183,484 - Pf1N1B4_4691 0.64 -1.4 5,183,696 - -2.1 5,183,728 + -0.4 5,183,728 + +0.2 5,183,837 + +0.3 5,183,921 + -0.9 5,183,961 - -0.9 5,184,005 + +0.7 5,184,005 + -0.9 5,184,116 - -0.1
Or see this region's nucleotide sequence