Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4118 and Pf1N1B4_4119 are separated by 486 nucleotides Pf1N1B4_4119 and Pf1N1B4_4120 are separated by 67 nucleotides Pf1N1B4_4120 and Pf1N1B4_4121 are separated by 101 nucleotides Pf1N1B4_4121 and Pf1N1B4_4122 are separated by 270 nucleotides
Pf1N1B4_4118 - Cyn operon transcriptional activator, at 4,560,664 to 4,561,593
_4118
Pf1N1B4_4119 - dioxygenase, at 4,562,080 to 4,562,391
_4119
Pf1N1B4_4120 - hypothetical protein, at 4,562,459 to 4,562,647
_4120
Pf1N1B4_4121 - putative lipoprotein, at 4,562,749 to 4,563,291
_4121
Pf1N1B4_4122 - Acetyltransferase, at 4,563,562 to 4,564,062
_4122
Position (kb)
4562
4563 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4561.592 kb on - strand at 4561.684 kb on - strand at 4561.742 kb on + strand at 4561.837 kb on + strand at 4561.838 kb on - strand at 4561.838 kb on - strand at 4561.838 kb on - strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.893 kb on - strand at 4561.893 kb on - strand at 4561.922 kb on + strand at 4561.975 kb on + strand at 4562.079 kb on + strand at 4562.084 kb on + strand at 4562.146 kb on + strand, within Pf1N1B4_4119 at 4562.147 kb on - strand, within Pf1N1B4_4119 at 4562.185 kb on + strand, within Pf1N1B4_4119 at 4562.186 kb on - strand, within Pf1N1B4_4119 at 4562.186 kb on - strand, within Pf1N1B4_4119 at 4562.279 kb on + strand, within Pf1N1B4_4119 at 4562.279 kb on + strand, within Pf1N1B4_4119 at 4562.417 kb on + strand at 4562.587 kb on + strand, within Pf1N1B4_4120 at 4562.588 kb on - strand, within Pf1N1B4_4120 at 4562.646 kb on - strand at 4562.646 kb on - strand at 4562.713 kb on + strand at 4562.714 kb on - strand at 4562.714 kb on - strand at 4562.829 kb on - strand, within Pf1N1B4_4121 at 4562.888 kb on - strand, within Pf1N1B4_4121 at 4562.888 kb on - strand, within Pf1N1B4_4121 at 4562.932 kb on + strand at 4562.932 kb on + strand at 4562.933 kb on - strand, within Pf1N1B4_4121 at 4562.933 kb on - strand at 4562.933 kb on - strand, within Pf1N1B4_4121 at 4562.936 kb on + strand, within Pf1N1B4_4121 at 4562.936 kb on + strand, within Pf1N1B4_4121 at 4562.936 kb on + strand, within Pf1N1B4_4121 at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.937 kb on - strand at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.937 kb on - strand, within Pf1N1B4_4121 at 4562.973 kb on - strand, within Pf1N1B4_4121 at 4562.979 kb on - strand, within Pf1N1B4_4121 at 4563.026 kb on + strand, within Pf1N1B4_4121 at 4563.027 kb on - strand, within Pf1N1B4_4121 at 4563.059 kb on - strand, within Pf1N1B4_4121 at 4563.059 kb on - strand, within Pf1N1B4_4121 at 4563.072 kb on - strand, within Pf1N1B4_4121 at 4563.170 kb on + strand, within Pf1N1B4_4121 at 4563.306 kb on + strand at 4563.310 kb on - strand at 4563.310 kb on - strand at 4563.398 kb on + strand at 4563.403 kb on + strand at 4563.404 kb on - strand at 4563.413 kb on - strand at 4563.564 kb on - strand at 4563.614 kb on - strand, within Pf1N1B4_4122
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 4,561,592 - -0.1 4,561,684 - +3.2 4,561,742 + -0.9 4,561,837 + +1.7 4,561,838 - -2.2 4,561,838 - +2.4 4,561,838 - +0.0 4,561,892 + -0.4 4,561,892 + -0.4 4,561,892 + +1.3 4,561,892 + +2.1 4,561,893 - -1.1 4,561,893 - -1.2 4,561,922 + -0.6 4,561,975 + -0.2 4,562,079 + -0.6 4,562,084 + -1.1 4,562,146 + Pf1N1B4_4119 0.21 +0.2 4,562,147 - Pf1N1B4_4119 0.21 -0.2 4,562,185 + Pf1N1B4_4119 0.34 -2.3 4,562,186 - Pf1N1B4_4119 0.34 -1.6 4,562,186 - Pf1N1B4_4119 0.34 -1.5 4,562,279 + Pf1N1B4_4119 0.64 -1.3 4,562,279 + Pf1N1B4_4119 0.64 -2.5 4,562,417 + +0.2 4,562,587 + Pf1N1B4_4120 0.68 +0.4 4,562,588 - Pf1N1B4_4120 0.68 +1.0 4,562,646 - -0.1 4,562,646 - +0.3 4,562,713 + -1.1 4,562,714 - -1.0 4,562,714 - +1.0 4,562,829 - Pf1N1B4_4121 0.15 -0.2 4,562,888 - Pf1N1B4_4121 0.26 -3.0 4,562,888 - Pf1N1B4_4121 0.26 +0.3 4,562,932 + +1.4 4,562,932 + +0.6 4,562,933 - Pf1N1B4_4121 0.34 -1.3 4,562,933 - +4.0 4,562,933 - Pf1N1B4_4121 0.34 -0.8 4,562,936 + Pf1N1B4_4121 0.34 -0.4 4,562,936 + Pf1N1B4_4121 0.34 +0.8 4,562,936 + Pf1N1B4_4121 0.34 +0.5 4,562,937 - Pf1N1B4_4121 0.35 +2.4 4,562,937 - Pf1N1B4_4121 0.35 -0.1 4,562,937 - Pf1N1B4_4121 0.35 +2.3 4,562,937 - +2.2 4,562,937 - Pf1N1B4_4121 0.35 +0.9 4,562,937 - Pf1N1B4_4121 0.35 -1.7 4,562,973 - Pf1N1B4_4121 0.41 -0.2 4,562,979 - Pf1N1B4_4121 0.42 -0.6 4,563,026 + Pf1N1B4_4121 0.51 -2.4 4,563,027 - Pf1N1B4_4121 0.51 +1.2 4,563,059 - Pf1N1B4_4121 0.57 -0.4 4,563,059 - Pf1N1B4_4121 0.57 -2.1 4,563,072 - Pf1N1B4_4121 0.59 +0.7 4,563,170 + Pf1N1B4_4121 0.78 -1.5 4,563,306 + +0.1 4,563,310 - -1.7 4,563,310 - +0.1 4,563,398 + +0.7 4,563,403 + -0.9 4,563,404 - -1.1 4,563,413 - -1.6 4,563,564 - -0.3 4,563,614 - Pf1N1B4_4122 0.10 -1.1
Or see this region's nucleotide sequence