Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3525 and Pf1N1B4_3526 are separated by 50 nucleotides Pf1N1B4_3526 and Pf1N1B4_3527 are separated by 8 nucleotides Pf1N1B4_3527 and Pf1N1B4_3528 are separated by 68 nucleotides
Pf1N1B4_3525 - hydrolase, alpha/beta fold family, at 3,931,039 to 3,931,893
_3525
Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603
_3526
Pf1N1B4_3527 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 3,932,612 to 3,933,418
_3527
Pf1N1B4_3528 - Putative membrane protein, at 3,933,487 to 3,934,542
_3528
Position (kb)
3932
3933
3934 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.630 kb on - strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.949 kb on - strand at 3931.949 kb on - strand at 3931.956 kb on + strand at 3931.956 kb on + strand at 3932.164 kb on - strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.403 kb on + strand, within Pf1N1B4_3526 at 3932.427 kb on + strand, within Pf1N1B4_3526 at 3932.608 kb on - strand at 3932.672 kb on - strand at 3932.849 kb on + strand at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.932 kb on - strand, within Pf1N1B4_3527 at 3932.932 kb on - strand, within Pf1N1B4_3527 at 3933.005 kb on + strand, within Pf1N1B4_3527 at 3933.030 kb on - strand, within Pf1N1B4_3527 at 3933.030 kb on - strand, within Pf1N1B4_3527 at 3933.034 kb on - strand, within Pf1N1B4_3527 at 3933.080 kb on + strand, within Pf1N1B4_3527 at 3933.081 kb on - strand, within Pf1N1B4_3527 at 3933.110 kb on + strand, within Pf1N1B4_3527 at 3933.351 kb on + strand at 3933.454 kb on - strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.485 kb on - strand at 3933.485 kb on - strand at 3933.773 kb on + strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.978 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.076 kb on - strand at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.353 kb on - strand at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.356 kb on + strand at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.357 kb on - strand, within Pf1N1B4_3528
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 3,931,629 + Pf1N1B4_3525 0.69 -2.1 3,931,629 + Pf1N1B4_3525 0.69 -1.2 3,931,630 - -0.4 3,931,948 + +0.9 3,931,948 + +0.3 3,931,948 + +1.9 3,931,949 - -0.7 3,931,949 - +1.3 3,931,956 + +0.4 3,931,956 + -2.4 3,932,164 - Pf1N1B4_3526 0.33 -2.5 3,932,352 + Pf1N1B4_3526 0.62 +0.1 3,932,352 + Pf1N1B4_3526 0.62 +1.3 3,932,352 + Pf1N1B4_3526 0.62 -1.8 3,932,352 + Pf1N1B4_3526 0.62 -0.1 3,932,352 + Pf1N1B4_3526 0.62 -0.9 3,932,352 + Pf1N1B4_3526 0.62 +0.1 3,932,352 + Pf1N1B4_3526 0.62 -2.0 3,932,403 + Pf1N1B4_3526 0.70 +0.0 3,932,427 + Pf1N1B4_3526 0.73 -1.3 3,932,608 - +0.2 3,932,672 - -0.4 3,932,849 + +0.6 3,932,850 - Pf1N1B4_3527 0.29 -2.0 3,932,850 - Pf1N1B4_3527 0.29 -1.3 3,932,850 - Pf1N1B4_3527 0.29 +1.1 3,932,932 - Pf1N1B4_3527 0.40 -2.3 3,932,932 - Pf1N1B4_3527 0.40 -0.9 3,933,005 + Pf1N1B4_3527 0.49 +0.5 3,933,030 - Pf1N1B4_3527 0.52 -1.6 3,933,030 - Pf1N1B4_3527 0.52 -0.4 3,933,034 - Pf1N1B4_3527 0.52 -0.3 3,933,080 + Pf1N1B4_3527 0.58 +0.8 3,933,081 - Pf1N1B4_3527 0.58 +1.0 3,933,110 + Pf1N1B4_3527 0.62 -3.2 3,933,351 + +1.4 3,933,454 - -2.1 3,933,484 + -1.5 3,933,484 + -2.3 3,933,484 + -1.2 3,933,484 + -1.4 3,933,484 + -2.0 3,933,485 - +1.7 3,933,485 - -0.8 3,933,773 + Pf1N1B4_3528 0.27 +0.0 3,933,774 - Pf1N1B4_3528 0.27 -0.2 3,933,774 - Pf1N1B4_3528 0.27 -1.0 3,933,774 - Pf1N1B4_3528 0.27 -0.2 3,933,978 + Pf1N1B4_3528 0.46 -1.1 3,934,075 + Pf1N1B4_3528 0.56 -1.5 3,934,075 + Pf1N1B4_3528 0.56 -1.2 3,934,075 + Pf1N1B4_3528 0.56 -0.3 3,934,075 + Pf1N1B4_3528 0.56 -0.3 3,934,075 + Pf1N1B4_3528 0.56 -1.3 3,934,076 - +0.0 3,934,352 + Pf1N1B4_3528 0.82 +0.9 3,934,352 + Pf1N1B4_3528 0.82 -1.3 3,934,352 + -0.6 3,934,352 + Pf1N1B4_3528 0.82 +0.6 3,934,352 + Pf1N1B4_3528 0.82 -0.5 3,934,352 + Pf1N1B4_3528 0.82 -1.5 3,934,352 + Pf1N1B4_3528 0.82 -2.2 3,934,353 - -1.3 3,934,356 + Pf1N1B4_3528 0.82 +0.8 3,934,356 + -1.2 3,934,356 + Pf1N1B4_3528 0.82 -1.2 3,934,356 + Pf1N1B4_3528 0.82 +2.2 3,934,357 - Pf1N1B4_3528 0.82 -0.3
Or see this region's nucleotide sequence