Experiment: Formate with Neomycin 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04345 and MMP_RS04350 are separated by 220 nucleotides MMP_RS04350 and MMP_RS04355 are separated by 14 nucleotides MMP_RS04355 and MMP_RS04360 are separated by 130 nucleotides MMP_RS04360 and MMP_RS04365 are separated by 28 nucleotides
MMP_RS04345: MMP_RS04345 - MFS transporter, at 829,722 to 831,026
_RS04345
MMP_RS04350: MMP_RS04350 - 2-hydroxyacyl-CoA dehydratase family protein, at 831,247 to 832,368
_RS04350
MMP_RS04355: MMP_RS04355 - ribonuclease H family protein, at 832,383 to 832,988
_RS04355
MMP_RS04360: MMP_RS04360 - hypothetical protein, at 833,119 to 833,310
_RS04360
MMP_RS04365: MMP_RS04365 - M48 family metallopeptidase, at 833,339 to 834,007
_RS04365
Position (kb)
831
832
833 Strain fitness (log2 ratio)
-2
-1
0
1 at 830.322 kb on - strand, within MMP_RS04345 at 830.323 kb on + strand, within MMP_RS04345 at 830.326 kb on + strand, within MMP_RS04345 at 830.353 kb on + strand, within MMP_RS04345 at 830.461 kb on + strand, within MMP_RS04345 at 830.461 kb on + strand, within MMP_RS04345 at 830.494 kb on + strand, within MMP_RS04345 at 830.507 kb on - strand, within MMP_RS04345 at 830.511 kb on - strand, within MMP_RS04345 at 830.527 kb on + strand, within MMP_RS04345 at 830.527 kb on + strand, within MMP_RS04345 at 830.597 kb on - strand, within MMP_RS04345 at 830.660 kb on - strand, within MMP_RS04345 at 830.662 kb on - strand, within MMP_RS04345 at 830.662 kb on - strand, within MMP_RS04345 at 830.663 kb on + strand, within MMP_RS04345 at 830.663 kb on + strand, within MMP_RS04345 at 830.729 kb on - strand, within MMP_RS04345 at 830.836 kb on - strand, within MMP_RS04345 at 830.862 kb on - strand, within MMP_RS04345 at 830.901 kb on + strand at 830.953 kb on - strand at 830.989 kb on - strand at 831.028 kb on - strand at 831.028 kb on - strand at 831.028 kb on - strand at 831.028 kb on - strand at 831.028 kb on - strand at 831.205 kb on + strand at 831.208 kb on + strand at 831.212 kb on - strand at 831.212 kb on - strand at 831.212 kb on - strand at 831.213 kb on + strand at 831.219 kb on - strand at 831.225 kb on + strand at 831.258 kb on + strand at 831.338 kb on - strand at 831.375 kb on - strand, within MMP_RS04350 at 831.376 kb on + strand, within MMP_RS04350 at 831.378 kb on + strand, within MMP_RS04350 at 831.463 kb on + strand, within MMP_RS04350 at 831.463 kb on + strand, within MMP_RS04350 at 831.477 kb on - strand, within MMP_RS04350 at 831.607 kb on - strand, within MMP_RS04350 at 831.686 kb on + strand, within MMP_RS04350 at 831.789 kb on + strand, within MMP_RS04350 at 831.871 kb on + strand, within MMP_RS04350 at 831.893 kb on - strand, within MMP_RS04350 at 831.894 kb on + strand, within MMP_RS04350 at 831.894 kb on + strand, within MMP_RS04350 at 831.934 kb on - strand, within MMP_RS04350 at 832.045 kb on + strand, within MMP_RS04350 at 832.071 kb on + strand, within MMP_RS04350 at 832.175 kb on - strand, within MMP_RS04350 at 832.204 kb on - strand, within MMP_RS04350 at 832.208 kb on + strand, within MMP_RS04350 at 832.214 kb on - strand, within MMP_RS04350 at 832.214 kb on - strand, within MMP_RS04350 at 832.220 kb on + strand, within MMP_RS04350 at 832.234 kb on + strand, within MMP_RS04350 at 832.290 kb on - strand at 832.291 kb on + strand at 832.367 kb on + strand at 832.386 kb on - strand at 832.424 kb on - strand at 832.466 kb on - strand, within MMP_RS04355 at 832.483 kb on - strand, within MMP_RS04355 at 832.484 kb on + strand, within MMP_RS04355 at 832.489 kb on + strand, within MMP_RS04355 at 832.489 kb on + strand, within MMP_RS04355 at 832.501 kb on + strand, within MMP_RS04355 at 832.501 kb on + strand, within MMP_RS04355 at 832.503 kb on + strand, within MMP_RS04355 at 832.504 kb on - strand, within MMP_RS04355 at 832.504 kb on - strand, within MMP_RS04355 at 832.504 kb on - strand, within MMP_RS04355 at 832.505 kb on + strand, within MMP_RS04355 at 832.505 kb on + strand, within MMP_RS04355 at 832.509 kb on - strand, within MMP_RS04355 at 832.510 kb on + strand, within MMP_RS04355 at 832.510 kb on + strand, within MMP_RS04355 at 832.511 kb on - strand, within MMP_RS04355 at 832.544 kb on - strand, within MMP_RS04355 at 832.567 kb on - strand, within MMP_RS04355 at 832.567 kb on - strand, within MMP_RS04355 at 832.568 kb on + strand, within MMP_RS04355 at 832.568 kb on + strand, within MMP_RS04355 at 832.576 kb on - strand, within MMP_RS04355 at 832.576 kb on - strand, within MMP_RS04355 at 832.576 kb on - strand, within MMP_RS04355 at 832.576 kb on - strand, within MMP_RS04355 at 832.577 kb on + strand, within MMP_RS04355 at 832.578 kb on - strand, within MMP_RS04355 at 832.578 kb on - strand, within MMP_RS04355 at 832.578 kb on - strand, within MMP_RS04355 at 832.578 kb on - strand, within MMP_RS04355 at 832.695 kb on + strand, within MMP_RS04355 at 832.788 kb on - strand, within MMP_RS04355 at 832.788 kb on - strand, within MMP_RS04355 at 832.938 kb on + strand at 832.940 kb on - strand at 832.945 kb on + strand at 832.945 kb on + strand at 832.975 kb on - strand at 832.975 kb on - strand at 832.977 kb on - strand at 832.977 kb on - strand at 832.978 kb on + strand at 832.983 kb on + strand at 833.004 kb on - strand at 833.018 kb on - strand at 833.018 kb on - strand at 833.018 kb on - strand at 833.018 kb on - strand at 833.019 kb on + strand at 833.020 kb on - strand at 833.021 kb on + strand at 833.023 kb on + strand at 833.023 kb on + strand at 833.023 kb on + strand at 833.027 kb on + strand at 833.055 kb on + strand at 833.103 kb on + strand at 833.178 kb on + strand, within MMP_RS04360 at 833.216 kb on - strand, within MMP_RS04360 at 833.216 kb on - strand, within MMP_RS04360 at 833.308 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Neomycin 12.5 mM remove 830,322 - MMP_RS04345 0.46 -0.1 830,323 + MMP_RS04345 0.46 +0.4 830,326 + MMP_RS04345 0.46 -0.0 830,353 + MMP_RS04345 0.48 -0.1 830,461 + MMP_RS04345 0.57 -0.2 830,461 + MMP_RS04345 0.57 -0.5 830,494 + MMP_RS04345 0.59 +0.1 830,507 - MMP_RS04345 0.60 +0.1 830,511 - MMP_RS04345 0.60 -0.2 830,527 + MMP_RS04345 0.62 +0.7 830,527 + MMP_RS04345 0.62 -0.4 830,597 - MMP_RS04345 0.67 -0.6 830,660 - MMP_RS04345 0.72 -0.1 830,662 - MMP_RS04345 0.72 +0.2 830,662 - MMP_RS04345 0.72 -0.0 830,663 + MMP_RS04345 0.72 +0.1 830,663 + MMP_RS04345 0.72 +0.0 830,729 - MMP_RS04345 0.77 -0.5 830,836 - MMP_RS04345 0.85 +0.0 830,862 - MMP_RS04345 0.87 -0.1 830,901 + +0.2 830,953 - -0.1 830,989 - -0.0 831,028 - -1.0 831,028 - -0.3 831,028 - +0.2 831,028 - -0.8 831,028 - -0.2 831,205 + -0.3 831,208 + -0.5 831,212 - -1.1 831,212 - +0.1 831,212 - +0.3 831,213 + -0.2 831,219 - -0.1 831,225 + +0.1 831,258 + +0.1 831,338 - -0.2 831,375 - MMP_RS04350 0.11 +0.3 831,376 + MMP_RS04350 0.11 -0.0 831,378 + MMP_RS04350 0.12 -0.5 831,463 + MMP_RS04350 0.19 -0.6 831,463 + MMP_RS04350 0.19 +0.3 831,477 - MMP_RS04350 0.20 -0.2 831,607 - MMP_RS04350 0.32 -0.7 831,686 + MMP_RS04350 0.39 +0.3 831,789 + MMP_RS04350 0.48 -0.1 831,871 + MMP_RS04350 0.56 +0.3 831,893 - MMP_RS04350 0.58 -0.8 831,894 + MMP_RS04350 0.58 -0.5 831,894 + MMP_RS04350 0.58 -0.3 831,934 - MMP_RS04350 0.61 +0.7 832,045 + MMP_RS04350 0.71 -0.1 832,071 + MMP_RS04350 0.73 -0.4 832,175 - MMP_RS04350 0.83 -1.8 832,204 - MMP_RS04350 0.85 +0.7 832,208 + MMP_RS04350 0.86 -0.4 832,214 - MMP_RS04350 0.86 -0.3 832,214 - MMP_RS04350 0.86 -0.6 832,220 + MMP_RS04350 0.87 +0.2 832,234 + MMP_RS04350 0.88 -0.7 832,290 - -1.3 832,291 + -0.4 832,367 + +0.2 832,386 - +1.2 832,424 - +0.1 832,466 - MMP_RS04355 0.14 +0.3 832,483 - MMP_RS04355 0.17 -0.4 832,484 + MMP_RS04355 0.17 +0.1 832,489 + MMP_RS04355 0.17 +0.4 832,489 + MMP_RS04355 0.17 +0.5 832,501 + MMP_RS04355 0.19 +0.2 832,501 + MMP_RS04355 0.19 -0.2 832,503 + MMP_RS04355 0.20 +0.1 832,504 - MMP_RS04355 0.20 -0.8 832,504 - MMP_RS04355 0.20 +0.1 832,504 - MMP_RS04355 0.20 -0.4 832,505 + MMP_RS04355 0.20 +1.0 832,505 + MMP_RS04355 0.20 -0.1 832,509 - MMP_RS04355 0.21 +0.3 832,510 + MMP_RS04355 0.21 -2.0 832,510 + MMP_RS04355 0.21 +0.2 832,511 - MMP_RS04355 0.21 +0.1 832,544 - MMP_RS04355 0.27 -0.2 832,567 - MMP_RS04355 0.30 -0.5 832,567 - MMP_RS04355 0.30 -0.6 832,568 + MMP_RS04355 0.31 -0.9 832,568 + MMP_RS04355 0.31 -0.1 832,576 - MMP_RS04355 0.32 -0.3 832,576 - MMP_RS04355 0.32 -0.6 832,576 - MMP_RS04355 0.32 -0.1 832,576 - MMP_RS04355 0.32 -0.3 832,577 + MMP_RS04355 0.32 -1.0 832,578 - MMP_RS04355 0.32 -2.0 832,578 - MMP_RS04355 0.32 +0.1 832,578 - MMP_RS04355 0.32 -0.2 832,578 - MMP_RS04355 0.32 -0.0 832,695 + MMP_RS04355 0.51 -0.5 832,788 - MMP_RS04355 0.67 -0.7 832,788 - MMP_RS04355 0.67 -0.5 832,938 + -0.6 832,940 - -0.2 832,945 + +0.2 832,945 + -0.2 832,975 - -0.4 832,975 - -0.5 832,977 - +0.3 832,977 - -0.4 832,978 + -0.4 832,983 + -0.0 833,004 - +0.3 833,018 - +0.0 833,018 - -0.1 833,018 - +0.4 833,018 - +0.8 833,019 + +0.0 833,020 - -0.1 833,021 + +0.4 833,023 + -1.1 833,023 + +0.2 833,023 + +0.4 833,027 + +0.3 833,055 + -0.6 833,103 + -0.0 833,178 + MMP_RS04360 0.31 +0.2 833,216 - MMP_RS04360 0.51 -0.2 833,216 - MMP_RS04360 0.51 +0.1 833,308 - +0.2
Or see this region's nucleotide sequence