Experiment: Formate with Neomycin 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04275 and MMP_RS04280 are separated by 16 nucleotides MMP_RS04280 and MMP_RS04285 are separated by 21 nucleotides MMP_RS04285 and MMP_RS04290 are separated by 8 nucleotides
MMP_RS04275: MMP_RS04275 - hydrogenase iron-sulfur subunit, at 815,353 to 815,775
_RS04275
MMP_RS04280: MMP_RS04280 - coenzyme F420-non-reducing hydrogenase subunit gamma, at 815,792 to 816,694
_RS04280
MMP_RS04285: MMP_RS04285 - Ni/Fe hydrogenase subunit alpha, at 816,716 to 818,131
_RS04285
MMP_RS04290: MMP_RS04290 - 4Fe-4S binding protein, at 818,140 to 819,333
_RS04290
Position (kb) 816
817
818
819 Strain fitness (log2 ratio) -1
0
1 at 815.770 kb on + strand at 815.778 kb on + strand at 815.778 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.807 kb on + strand at 815.807 kb on + strand at 815.841 kb on - strand at 815.841 kb on - strand at 815.842 kb on + strand at 815.856 kb on - strand at 815.856 kb on - strand at 816.082 kb on + strand, within MMP_RS04280 at 816.121 kb on - strand, within MMP_RS04280 at 816.268 kb on + strand, within MMP_RS04280 at 816.310 kb on - strand, within MMP_RS04280 at 816.449 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.496 kb on - strand, within MMP_RS04280 at 816.520 kb on - strand, within MMP_RS04280 at 816.542 kb on + strand, within MMP_RS04280 at 816.570 kb on + strand, within MMP_RS04280 at 816.692 kb on - strand at 816.951 kb on - strand, within MMP_RS04285 at 817.135 kb on + strand, within MMP_RS04285 at 817.417 kb on - strand, within MMP_RS04285 at 817.417 kb on - strand, within MMP_RS04285 at 817.422 kb on + strand, within MMP_RS04285 at 817.527 kb on + strand, within MMP_RS04285 at 817.640 kb on - strand, within MMP_RS04285 at 817.644 kb on + strand, within MMP_RS04285 at 817.752 kb on - strand, within MMP_RS04285 at 817.752 kb on - strand, within MMP_RS04285 at 817.782 kb on - strand, within MMP_RS04285 at 817.968 kb on - strand, within MMP_RS04285 at 818.170 kb on + strand at 818.262 kb on + strand, within MMP_RS04290 at 818.301 kb on + strand, within MMP_RS04290 at 818.367 kb on - strand, within MMP_RS04290 at 818.506 kb on + strand, within MMP_RS04290 at 818.631 kb on - strand, within MMP_RS04290 at 818.674 kb on + strand, within MMP_RS04290 at 818.766 kb on - strand, within MMP_RS04290 at 818.850 kb on - strand, within MMP_RS04290 at 818.927 kb on - strand, within MMP_RS04290 at 818.972 kb on - strand, within MMP_RS04290 at 819.115 kb on + strand, within MMP_RS04290
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Neomycin 12.5 mM remove 815,770 + -0.1 815,778 + +0.1 815,778 + -0.6 815,781 + +0.2 815,781 + -0.5 815,781 + -0.1 815,781 + -1.2 815,807 + -0.2 815,807 + -0.5 815,841 - +0.1 815,841 - +0.1 815,842 + +0.1 815,856 - -0.2 815,856 - +0.4 816,082 + MMP_RS04280 0.32 +0.2 816,121 - MMP_RS04280 0.36 -0.3 816,268 + MMP_RS04280 0.53 +1.1 816,310 - MMP_RS04280 0.57 -0.2 816,449 + MMP_RS04280 0.73 -0.2 816,472 + MMP_RS04280 0.75 -1.0 816,472 + MMP_RS04280 0.75 +0.3 816,472 + MMP_RS04280 0.75 +0.1 816,496 - MMP_RS04280 0.78 +0.2 816,520 - MMP_RS04280 0.81 -0.3 816,542 + MMP_RS04280 0.83 +0.2 816,570 + MMP_RS04280 0.86 +0.1 816,692 - +0.1 816,951 - MMP_RS04285 0.17 -0.4 817,135 + MMP_RS04285 0.30 -0.4 817,417 - MMP_RS04285 0.50 -0.1 817,417 - MMP_RS04285 0.50 -0.2 817,422 + MMP_RS04285 0.50 -0.1 817,527 + MMP_RS04285 0.57 +0.8 817,640 - MMP_RS04285 0.65 -0.2 817,644 + MMP_RS04285 0.66 -0.3 817,752 - MMP_RS04285 0.73 +0.3 817,752 - MMP_RS04285 0.73 -0.8 817,782 - MMP_RS04285 0.75 -0.6 817,968 - MMP_RS04285 0.88 -0.6 818,170 + +0.3 818,262 + MMP_RS04290 0.10 +1.0 818,301 + MMP_RS04290 0.13 +0.3 818,367 - MMP_RS04290 0.19 +0.2 818,506 + MMP_RS04290 0.31 +0.1 818,631 - MMP_RS04290 0.41 -0.1 818,674 + MMP_RS04290 0.45 -0.2 818,766 - MMP_RS04290 0.52 -0.5 818,850 - MMP_RS04290 0.59 -0.0 818,927 - MMP_RS04290 0.66 +0.1 818,972 - MMP_RS04290 0.70 -0.2 819,115 + MMP_RS04290 0.82 -0.3
Or see this region's nucleotide sequence