Experiment: Survival; Treatment=FW106 (pH3.9); TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_15395 and IAI47_15400 are separated by 128 nucleotides IAI47_15400 and IAI47_15405 overlap by 4 nucleotides IAI47_15405 and IAI47_15410 are separated by 89 nucleotides
IAI47_15395: IAI47_15395 - MetQ/NlpA family lipoprotein, at 3,242,555 to 3,243,370
_15395
IAI47_15400: IAI47_15400 - Rcs stress response system protein RcsF, at 3,243,499 to 3,243,897
_15400
IAI47_15405: IAI47_15405 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 3,243,894 to 3,244,598
_15405
IAI47_15410: IAI47_15410 - proline--tRNA ligase, at 3,244,688 to 3,246,406
_15410
Position (kb)
3243
3244
3245 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 3242.986 kb on + strand, within IAI47_15395 at 3243.076 kb on + strand, within IAI47_15395 at 3243.076 kb on + strand, within IAI47_15395 at 3243.077 kb on - strand, within IAI47_15395 at 3243.167 kb on + strand, within IAI47_15395 at 3243.167 kb on + strand, within IAI47_15395 at 3243.167 kb on + strand, within IAI47_15395 at 3243.168 kb on - strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.233 kb on + strand, within IAI47_15395 at 3243.234 kb on - strand, within IAI47_15395 at 3243.234 kb on - strand, within IAI47_15395 at 3243.234 kb on - strand, within IAI47_15395 at 3243.234 kb on - strand, within IAI47_15395 at 3243.234 kb on - strand, within IAI47_15395 at 3243.234 kb on - strand, within IAI47_15395 at 3243.234 kb on - strand, within IAI47_15395 at 3243.234 kb on - strand, within IAI47_15395 at 3243.262 kb on + strand, within IAI47_15395 at 3243.262 kb on + strand, within IAI47_15395 at 3243.262 kb on + strand, within IAI47_15395 at 3243.263 kb on - strand, within IAI47_15395 at 3243.263 kb on - strand, within IAI47_15395 at 3243.265 kb on + strand, within IAI47_15395 at 3243.265 kb on + strand, within IAI47_15395 at 3243.265 kb on + strand, within IAI47_15395 at 3243.265 kb on + strand, within IAI47_15395 at 3243.266 kb on - strand, within IAI47_15395 at 3243.266 kb on - strand, within IAI47_15395 at 3243.266 kb on - strand, within IAI47_15395 at 3243.302 kb on + strand at 3243.303 kb on - strand at 3243.320 kb on - strand at 3243.331 kb on + strand at 3243.331 kb on + strand at 3243.332 kb on - strand at 3243.332 kb on - strand at 3243.332 kb on - strand at 3243.334 kb on + strand at 3243.334 kb on + strand at 3243.335 kb on - strand at 3243.335 kb on - strand at 3243.368 kb on + strand at 3243.390 kb on - strand at 3243.402 kb on - strand at 3243.418 kb on + strand at 3243.418 kb on + strand at 3243.419 kb on - strand at 3243.419 kb on - strand at 3243.442 kb on + strand at 3243.442 kb on + strand at 3243.443 kb on - strand at 3243.443 kb on - strand at 3243.443 kb on - strand at 3243.446 kb on + strand at 3243.446 kb on + strand at 3243.446 kb on + strand at 3243.446 kb on + strand at 3243.447 kb on - strand at 3243.447 kb on - strand at 3243.447 kb on - strand at 3243.447 kb on - strand at 3243.525 kb on - strand at 3243.525 kb on - strand at 3243.525 kb on - strand at 3243.556 kb on + strand, within IAI47_15400 at 3243.556 kb on + strand, within IAI47_15400 at 3243.556 kb on + strand, within IAI47_15400 at 3243.556 kb on + strand, within IAI47_15400 at 3243.557 kb on - strand, within IAI47_15400 at 3243.612 kb on + strand, within IAI47_15400 at 3243.612 kb on + strand, within IAI47_15400 at 3243.613 kb on - strand, within IAI47_15400 at 3243.613 kb on - strand, within IAI47_15400 at 3243.646 kb on + strand, within IAI47_15400 at 3243.646 kb on + strand, within IAI47_15400 at 3243.646 kb on + strand, within IAI47_15400 at 3243.647 kb on - strand, within IAI47_15400 at 3243.647 kb on - strand, within IAI47_15400 at 3243.648 kb on + strand, within IAI47_15400 at 3243.649 kb on - strand, within IAI47_15400 at 3243.649 kb on - strand, within IAI47_15400 at 3243.672 kb on + strand, within IAI47_15400 at 3243.672 kb on + strand, within IAI47_15400 at 3243.750 kb on + strand, within IAI47_15400 at 3243.751 kb on - strand, within IAI47_15400 at 3243.848 kb on - strand, within IAI47_15400 at 3244.017 kb on + strand, within IAI47_15405 at 3244.017 kb on + strand, within IAI47_15405 at 3244.017 kb on + strand, within IAI47_15405 at 3244.017 kb on + strand, within IAI47_15405 at 3244.017 kb on + strand, within IAI47_15405 at 3244.017 kb on + strand, within IAI47_15405 at 3244.017 kb on + strand, within IAI47_15405 at 3244.017 kb on + strand, within IAI47_15405 at 3244.018 kb on - strand, within IAI47_15405 at 3244.018 kb on - strand, within IAI47_15405 at 3244.018 kb on - strand, within IAI47_15405 at 3244.018 kb on - strand, within IAI47_15405 at 3244.018 kb on - strand, within IAI47_15405 at 3244.018 kb on - strand, within IAI47_15405 at 3244.295 kb on + strand, within IAI47_15405 at 3244.305 kb on + strand, within IAI47_15405 at 3244.305 kb on + strand, within IAI47_15405 at 3244.305 kb on + strand, within IAI47_15405 at 3244.305 kb on + strand, within IAI47_15405 at 3244.306 kb on - strand, within IAI47_15405 at 3244.306 kb on - strand, within IAI47_15405 at 3244.306 kb on - strand, within IAI47_15405 at 3244.306 kb on - strand, within IAI47_15405 at 3244.306 kb on - strand, within IAI47_15405 at 3244.373 kb on + strand, within IAI47_15405 at 3244.374 kb on - strand, within IAI47_15405 at 3244.374 kb on - strand, within IAI47_15405 at 3244.374 kb on - strand, within IAI47_15405 at 3244.515 kb on + strand, within IAI47_15405 at 3244.629 kb on + strand at 3244.629 kb on + strand at 3244.629 kb on + strand at 3244.629 kb on + strand at 3244.634 kb on + strand at 3244.634 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW106 (pH3.9); TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 3,242,986 + IAI47_15395 0.53 -1.2 3,243,076 + IAI47_15395 0.64 +0.1 3,243,076 + IAI47_15395 0.64 -2.2 3,243,077 - IAI47_15395 0.64 -1.7 3,243,167 + IAI47_15395 0.75 +1.2 3,243,167 + IAI47_15395 0.75 +0.4 3,243,167 + IAI47_15395 0.75 -2.4 3,243,168 - IAI47_15395 0.75 -1.5 3,243,233 + IAI47_15395 0.83 -2.7 3,243,233 + IAI47_15395 0.83 +4.3 3,243,233 + IAI47_15395 0.83 +2.2 3,243,233 + IAI47_15395 0.83 -0.5 3,243,233 + IAI47_15395 0.83 +1.6 3,243,233 + IAI47_15395 0.83 -0.2 3,243,233 + IAI47_15395 0.83 -0.4 3,243,233 + IAI47_15395 0.83 +1.6 3,243,233 + IAI47_15395 0.83 +1.1 3,243,234 - IAI47_15395 0.83 -2.3 3,243,234 - IAI47_15395 0.83 +3.1 3,243,234 - IAI47_15395 0.83 +0.1 3,243,234 - IAI47_15395 0.83 +0.6 3,243,234 - IAI47_15395 0.83 -0.9 3,243,234 - IAI47_15395 0.83 +1.3 3,243,234 - IAI47_15395 0.83 -0.8 3,243,234 - IAI47_15395 0.83 +0.6 3,243,262 + IAI47_15395 0.87 -1.7 3,243,262 + IAI47_15395 0.87 +2.6 3,243,262 + IAI47_15395 0.87 -0.7 3,243,263 - IAI47_15395 0.87 +1.1 3,243,263 - IAI47_15395 0.87 +3.0 3,243,265 + IAI47_15395 0.87 +3.9 3,243,265 + IAI47_15395 0.87 -0.8 3,243,265 + IAI47_15395 0.87 -1.6 3,243,265 + IAI47_15395 0.87 +0.5 3,243,266 - IAI47_15395 0.87 -1.3 3,243,266 - IAI47_15395 0.87 +1.7 3,243,266 - IAI47_15395 0.87 -1.3 3,243,302 + -1.2 3,243,303 - -1.5 3,243,320 - +2.8 3,243,331 + -1.9 3,243,331 + -0.8 3,243,332 - +0.9 3,243,332 - -2.1 3,243,332 - -2.3 3,243,334 + -0.5 3,243,334 + +7.2 3,243,335 - +3.9 3,243,335 - +0.1 3,243,368 + +1.3 3,243,390 - +2.9 3,243,402 - -3.8 3,243,418 + -1.4 3,243,418 + -2.1 3,243,419 - -0.7 3,243,419 - -2.9 3,243,442 + -0.4 3,243,442 + -1.7 3,243,443 - +2.8 3,243,443 - -0.8 3,243,443 - +0.3 3,243,446 + -1.2 3,243,446 + +0.6 3,243,446 + -1.1 3,243,446 + -0.5 3,243,447 - -0.2 3,243,447 - +3.2 3,243,447 - +0.5 3,243,447 - +1.3 3,243,525 - +1.3 3,243,525 - -0.2 3,243,525 - +1.1 3,243,556 + IAI47_15400 0.14 +1.4 3,243,556 + IAI47_15400 0.14 -1.4 3,243,556 + IAI47_15400 0.14 +0.9 3,243,556 + IAI47_15400 0.14 -0.0 3,243,557 - IAI47_15400 0.15 +2.9 3,243,612 + IAI47_15400 0.28 -2.1 3,243,612 + IAI47_15400 0.28 +1.2 3,243,613 - IAI47_15400 0.29 +4.7 3,243,613 - IAI47_15400 0.29 +1.6 3,243,646 + IAI47_15400 0.37 +0.1 3,243,646 + IAI47_15400 0.37 +3.4 3,243,646 + IAI47_15400 0.37 -2.4 3,243,647 - IAI47_15400 0.37 +1.7 3,243,647 - IAI47_15400 0.37 -0.7 3,243,648 + IAI47_15400 0.37 +1.2 3,243,649 - IAI47_15400 0.38 -0.0 3,243,649 - IAI47_15400 0.38 -2.4 3,243,672 + IAI47_15400 0.43 +2.8 3,243,672 + IAI47_15400 0.43 -0.8 3,243,750 + IAI47_15400 0.63 -1.1 3,243,751 - IAI47_15400 0.63 -2.8 3,243,848 - IAI47_15400 0.87 +1.6 3,244,017 + IAI47_15405 0.17 +0.7 3,244,017 + IAI47_15405 0.17 +0.4 3,244,017 + IAI47_15405 0.17 -0.2 3,244,017 + IAI47_15405 0.17 -2.2 3,244,017 + IAI47_15405 0.17 +0.7 3,244,017 + IAI47_15405 0.17 +1.9 3,244,017 + IAI47_15405 0.17 +2.7 3,244,017 + IAI47_15405 0.17 +2.5 3,244,018 - IAI47_15405 0.18 -2.0 3,244,018 - IAI47_15405 0.18 +2.3 3,244,018 - IAI47_15405 0.18 +0.3 3,244,018 - IAI47_15405 0.18 +0.1 3,244,018 - IAI47_15405 0.18 -0.1 3,244,018 - IAI47_15405 0.18 +2.7 3,244,295 + IAI47_15405 0.57 -0.5 3,244,305 + IAI47_15405 0.58 +2.0 3,244,305 + IAI47_15405 0.58 -1.1 3,244,305 + IAI47_15405 0.58 +0.3 3,244,305 + IAI47_15405 0.58 -3.7 3,244,306 - IAI47_15405 0.58 +3.4 3,244,306 - IAI47_15405 0.58 +2.7 3,244,306 - IAI47_15405 0.58 +3.3 3,244,306 - IAI47_15405 0.58 +2.7 3,244,306 - IAI47_15405 0.58 -1.4 3,244,373 + IAI47_15405 0.68 +1.8 3,244,374 - IAI47_15405 0.68 +5.1 3,244,374 - IAI47_15405 0.68 +2.2 3,244,374 - IAI47_15405 0.68 +0.5 3,244,515 + IAI47_15405 0.88 -2.7 3,244,629 + -1.2 3,244,629 + -0.4 3,244,629 + +1.1 3,244,629 + -0.8 3,244,634 + +1.8 3,244,634 + +2.0
Or see this region's nucleotide sequence