Experiment: ALP with nitrite 0.45 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2954 and Dsui_2955 are separated by 803 nucleotides Dsui_2955 and Dsui_2956 overlap by 4 nucleotides Dsui_2956 and Dsui_2957 overlap by 7 nucleotides
Dsui_2954: Dsui_2954 - acyl-CoA hydrolase, at 3,162,961 to 3,163,830
_2954
Dsui_2955: Dsui_2955 - cobalamin biosynthesis protein CbiM, at 3,164,634 to 3,165,311
_2955
Dsui_2956: Dsui_2956 - ABC-type cobalt transport system, periplasmic component, at 3,165,308 to 3,165,679
_2956
Dsui_2957: Dsui_2957 - cobalt ABC transporter, permease protein CbiQ, at 3,165,673 to 3,166,404
_2957
Position (kb)
3164
3165
3166 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3163.947 kb on - strand at 3163.986 kb on - strand at 3164.011 kb on - strand at 3164.216 kb on - strand at 3164.318 kb on - strand at 3164.330 kb on - strand at 3164.362 kb on + strand at 3164.362 kb on + strand at 3164.370 kb on - strand at 3164.419 kb on - strand at 3164.454 kb on + strand at 3164.625 kb on - strand at 3164.639 kb on + strand at 3164.790 kb on - strand at 3165.119 kb on + strand, within Dsui_2955 at 3165.119 kb on + strand, within Dsui_2955 at 3165.240 kb on + strand, within Dsui_2955 at 3165.259 kb on + strand at 3165.367 kb on - strand, within Dsui_2956 at 3165.575 kb on + strand, within Dsui_2956 at 3165.650 kb on + strand at 3166.105 kb on - strand, within Dsui_2957 at 3166.105 kb on - strand at 3166.154 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrite 0.45 mM remove 3,163,947 - -1.1 3,163,986 - +0.0 3,164,011 - -0.0 3,164,216 - +0.0 3,164,318 - -1.1 3,164,330 - +0.5 3,164,362 + +0.4 3,164,362 + -0.0 3,164,370 - -0.5 3,164,419 - -2.1 3,164,454 + +0.2 3,164,625 - -0.5 3,164,639 + -0.1 3,164,790 - -0.5 3,165,119 + Dsui_2955 0.72 +0.4 3,165,119 + Dsui_2955 0.72 +0.2 3,165,240 + Dsui_2955 0.89 -0.1 3,165,259 + +2.8 3,165,367 - Dsui_2956 0.16 -0.5 3,165,575 + Dsui_2956 0.72 +0.2 3,165,650 + +0.1 3,166,105 - Dsui_2957 0.59 +0.5 3,166,105 - +0.5 3,166,154 - -1.1
Or see this region's nucleotide sequence