Experiment: ALP with nitrite 0.45 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2040 and Dsui_2041 are separated by 60 nucleotides Dsui_2041 and Dsui_2042 are separated by 20 nucleotides Dsui_2042 and Dsui_2043 are separated by 173 nucleotides
Dsui_2040: Dsui_2040 - putative CoA-binding protein, at 2,159,442 to 2,159,900
_2040
Dsui_2041: Dsui_2041 - enoyl-CoA hydratase/carnithine racemase, at 2,159,961 to 2,160,746
_2041
Dsui_2042: Dsui_2042 - cystathionine beta-lyase/cystathionine gamma-synthase, at 2,160,767 to 2,161,945
_2042
Dsui_2043: Dsui_2043 - cysteine synthase, at 2,162,119 to 2,163,624
_2043
Position (kb)
2160
2161
2162 Strain fitness (log2 ratio)
-1
0
1 at 2159.854 kb on - strand, within Dsui_2040 at 2159.872 kb on + strand at 2159.876 kb on + strand at 2159.880 kb on - strand at 2159.945 kb on + strand at 2159.951 kb on - strand at 2159.978 kb on - strand at 2160.064 kb on + strand, within Dsui_2041 at 2160.110 kb on - strand, within Dsui_2041 at 2160.117 kb on - strand, within Dsui_2041 at 2160.119 kb on + strand at 2160.220 kb on - strand, within Dsui_2041 at 2160.246 kb on - strand, within Dsui_2041 at 2160.261 kb on + strand at 2160.269 kb on - strand, within Dsui_2041 at 2160.277 kb on - strand at 2160.324 kb on - strand, within Dsui_2041 at 2160.324 kb on - strand at 2160.460 kb on + strand at 2160.470 kb on + strand at 2160.506 kb on - strand, within Dsui_2041 at 2160.592 kb on - strand at 2160.625 kb on + strand, within Dsui_2041 at 2160.696 kb on - strand at 2160.765 kb on - strand at 2160.789 kb on - strand at 2160.789 kb on - strand at 2160.792 kb on + strand at 2160.792 kb on + strand at 2160.868 kb on + strand at 2160.876 kb on - strand at 2160.876 kb on - strand at 2160.996 kb on + strand at 2161.004 kb on - strand at 2161.041 kb on + strand at 2161.080 kb on + strand at 2161.173 kb on - strand, within Dsui_2042 at 2161.211 kb on + strand, within Dsui_2042 at 2161.305 kb on - strand, within Dsui_2042 at 2161.401 kb on + strand at 2161.565 kb on - strand, within Dsui_2042 at 2161.610 kb on + strand, within Dsui_2042 at 2161.610 kb on + strand at 2161.636 kb on - strand, within Dsui_2042 at 2161.674 kb on - strand at 2161.705 kb on - strand, within Dsui_2042 at 2161.771 kb on + strand, within Dsui_2042 at 2161.798 kb on - strand, within Dsui_2042 at 2161.881 kb on + strand at 2161.941 kb on + strand at 2161.941 kb on + strand at 2161.976 kb on + strand at 2161.994 kb on + strand at 2162.250 kb on - strand at 2162.782 kb on - strand, within Dsui_2043 at 2162.862 kb on + strand, within Dsui_2043
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrite 0.45 mM remove 2,159,854 - Dsui_2040 0.90 +0.4 2,159,872 + -0.1 2,159,876 + +0.9 2,159,880 - -0.2 2,159,945 + -0.1 2,159,951 - +0.4 2,159,978 - +0.4 2,160,064 + Dsui_2041 0.13 -1.1 2,160,110 - Dsui_2041 0.19 -0.7 2,160,117 - Dsui_2041 0.20 +0.1 2,160,119 + +0.9 2,160,220 - Dsui_2041 0.33 +0.9 2,160,246 - Dsui_2041 0.36 -0.1 2,160,261 + +0.9 2,160,269 - Dsui_2041 0.39 +0.9 2,160,277 - -0.1 2,160,324 - Dsui_2041 0.46 +0.9 2,160,324 - +0.9 2,160,460 + -0.1 2,160,470 + +0.9 2,160,506 - Dsui_2041 0.69 +0.9 2,160,592 - +0.9 2,160,625 + Dsui_2041 0.84 -1.1 2,160,696 - +0.9 2,160,765 - -1.1 2,160,789 - +0.9 2,160,789 - -0.1 2,160,792 + +0.9 2,160,792 + -1.4 2,160,868 + -0.1 2,160,876 - +0.9 2,160,876 - +0.9 2,160,996 + +0.9 2,161,004 - +0.9 2,161,041 + +0.9 2,161,080 + +0.9 2,161,173 - Dsui_2042 0.34 -1.4 2,161,211 + Dsui_2042 0.38 -0.7 2,161,305 - Dsui_2042 0.46 -1.1 2,161,401 + +0.9 2,161,565 - Dsui_2042 0.68 -0.7 2,161,610 + Dsui_2042 0.72 -0.1 2,161,610 + -0.7 2,161,636 - Dsui_2042 0.74 -0.1 2,161,674 - +0.9 2,161,705 - Dsui_2042 0.80 +0.9 2,161,771 + Dsui_2042 0.85 +0.9 2,161,798 - Dsui_2042 0.87 +0.9 2,161,881 + +0.9 2,161,941 + +0.9 2,161,941 + +0.9 2,161,976 + -1.1 2,161,994 + +0.9 2,162,250 - +0.9 2,162,782 - Dsui_2043 0.44 +0.9 2,162,862 + Dsui_2043 0.49 +0.9
Or see this region's nucleotide sequence