Experiment: ALP with nitrite 0.45 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1295 and Dsui_1296 are separated by 21 nucleotides Dsui_1296 and Dsui_1297 are separated by 85 nucleotides Dsui_1297 and Dsui_1298 are separated by 5 nucleotides Dsui_1298 and Dsui_1299 are separated by 69 nucleotides
Dsui_1295: Dsui_1295 - lipid A core-O-antigen ligase-like enyme, at 1,357,876 to 1,359,690
_1295
Dsui_1296: Dsui_1296 - putative amidophosphoribosyltransferase, at 1,359,712 to 1,360,437
_1296
Dsui_1297: Dsui_1297 - biotin synthetase, at 1,360,523 to 1,361,530
_1297
Dsui_1298: Dsui_1298 - methylase involved in ubiquinone/menaquinone biosynthesis, at 1,361,536 to 1,362,438
_1298
Dsui_1299: Dsui_1299 - putative membrane protein, at 1,362,508 to 1,363,209
_1299
Position (kb)
1360
1361
1362 Strain fitness (log2 ratio)
-2
-1
0
1 at 1359.531 kb on + strand at 1359.552 kb on + strand at 1359.566 kb on + strand at 1359.566 kb on + strand at 1359.574 kb on - strand at 1359.604 kb on - strand at 1359.622 kb on + strand at 1359.624 kb on - strand at 1359.648 kb on + strand at 1359.658 kb on + strand at 1359.666 kb on - strand at 1359.756 kb on - strand at 1359.842 kb on - strand, within Dsui_1296 at 1359.906 kb on + strand, within Dsui_1296 at 1359.914 kb on - strand, within Dsui_1296 at 1359.970 kb on - strand at 1359.984 kb on - strand, within Dsui_1296 at 1360.080 kb on - strand, within Dsui_1296 at 1360.124 kb on - strand, within Dsui_1296 at 1360.129 kb on - strand, within Dsui_1296 at 1360.226 kb on - strand, within Dsui_1296 at 1360.238 kb on - strand, within Dsui_1296 at 1360.402 kb on + strand at 1360.410 kb on + strand at 1360.410 kb on - strand at 1360.918 kb on + strand, within Dsui_1297 at 1361.279 kb on + strand, within Dsui_1297 at 1361.290 kb on + strand, within Dsui_1297 at 1361.355 kb on + strand, within Dsui_1297 at 1362.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with nitrite 0.45 mM remove 1,359,531 + -0.6 1,359,552 + -0.6 1,359,566 + +0.4 1,359,566 + +0.4 1,359,574 - -2.1 1,359,604 - -2.1 1,359,622 + +0.4 1,359,624 - -2.7 1,359,648 + -1.1 1,359,658 + +0.4 1,359,666 - -0.6 1,359,756 - +0.4 1,359,842 - Dsui_1296 0.18 +0.4 1,359,906 + Dsui_1296 0.27 -1.6 1,359,914 - Dsui_1296 0.28 +0.4 1,359,970 - -0.6 1,359,984 - Dsui_1296 0.37 -1.6 1,360,080 - Dsui_1296 0.51 +0.4 1,360,124 - Dsui_1296 0.57 -1.6 1,360,129 - Dsui_1296 0.57 -0.6 1,360,226 - Dsui_1296 0.71 -2.6 1,360,238 - Dsui_1296 0.72 +0.4 1,360,402 + +0.4 1,360,410 + +0.4 1,360,410 - +0.4 1,360,918 + Dsui_1297 0.39 -0.0 1,361,279 + Dsui_1297 0.75 -0.1 1,361,290 + Dsui_1297 0.76 -0.2 1,361,355 + Dsui_1297 0.83 +0.0 1,362,487 - -0.9
Or see this region's nucleotide sequence