Experiment: sodium carbonate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4971 and Pf6N2E2_4972 are separated by 195 nucleotides Pf6N2E2_4972 and Pf6N2E2_4973 are separated by 111 nucleotides Pf6N2E2_4973 and Pf6N2E2_4974 are separated by 6 nucleotides
Pf6N2E2_4971 - Transcriptional regulator, at 5,736,619 to 5,737,350
_4971
Pf6N2E2_4972 - Inorganic pyrophosphatase (EC 3.6.1.1), at 5,737,546 to 5,738,073
_4972
Pf6N2E2_4973 - Inner membrane protein, at 5,738,185 to 5,738,997
_4973
Pf6N2E2_4974 - DedA protein, at 5,739,004 to 5,739,651
_4974
Position (kb)
5738
5739 Strain fitness (log2 ratio)
-2
-1
0
1 at 5737.246 kb on + strand, within Pf6N2E2_4971 at 5737.247 kb on - strand, within Pf6N2E2_4971 at 5737.247 kb on - strand, within Pf6N2E2_4971 at 5737.268 kb on + strand, within Pf6N2E2_4971 at 5737.268 kb on + strand, within Pf6N2E2_4971 at 5737.269 kb on - strand, within Pf6N2E2_4971 at 5737.310 kb on - strand at 5737.387 kb on + strand at 5737.407 kb on - strand at 5737.408 kb on + strand at 5737.408 kb on + strand at 5737.409 kb on - strand at 5737.409 kb on - strand at 5737.409 kb on - strand at 5737.409 kb on - strand at 5737.495 kb on + strand at 5737.502 kb on + strand at 5738.139 kb on - strand at 5738.139 kb on - strand at 5738.139 kb on - strand at 5738.145 kb on - strand at 5738.145 kb on - strand at 5738.178 kb on - strand at 5738.186 kb on + strand at 5738.186 kb on + strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.202 kb on - strand at 5738.256 kb on - strand at 5738.276 kb on + strand, within Pf6N2E2_4973 at 5738.276 kb on + strand, within Pf6N2E2_4973 at 5738.277 kb on - strand, within Pf6N2E2_4973 at 5738.277 kb on - strand, within Pf6N2E2_4973 at 5738.277 kb on - strand, within Pf6N2E2_4973 at 5738.277 kb on - strand, within Pf6N2E2_4973 at 5738.325 kb on - strand, within Pf6N2E2_4973 at 5738.325 kb on - strand, within Pf6N2E2_4973 at 5738.325 kb on - strand, within Pf6N2E2_4973 at 5738.325 kb on - strand, within Pf6N2E2_4973 at 5738.370 kb on - strand, within Pf6N2E2_4973 at 5738.552 kb on + strand, within Pf6N2E2_4973 at 5738.552 kb on + strand, within Pf6N2E2_4973 at 5738.729 kb on + strand, within Pf6N2E2_4973 at 5739.137 kb on + strand, within Pf6N2E2_4974 at 5739.137 kb on + strand, within Pf6N2E2_4974 at 5739.138 kb on - strand, within Pf6N2E2_4974 at 5739.207 kb on - strand, within Pf6N2E2_4974 at 5739.207 kb on - strand, within Pf6N2E2_4974 at 5739.207 kb on - strand, within Pf6N2E2_4974 at 5739.207 kb on - strand, within Pf6N2E2_4974 at 5739.302 kb on + strand, within Pf6N2E2_4974 at 5739.302 kb on + strand, within Pf6N2E2_4974 at 5739.326 kb on + strand, within Pf6N2E2_4974 at 5739.326 kb on + strand, within Pf6N2E2_4974 at 5739.395 kb on + strand, within Pf6N2E2_4974 at 5739.396 kb on - strand, within Pf6N2E2_4974 at 5739.396 kb on - strand, within Pf6N2E2_4974 at 5739.396 kb on - strand, within Pf6N2E2_4974 at 5739.396 kb on - strand, within Pf6N2E2_4974 at 5739.570 kb on - strand, within Pf6N2E2_4974 at 5739.584 kb on + strand, within Pf6N2E2_4974 at 5739.608 kb on + strand at 5739.608 kb on + strand at 5739.608 kb on + strand at 5739.655 kb on + strand at 5739.655 kb on + strand at 5739.655 kb on + strand at 5739.655 kb on + strand at 5739.655 kb on + strand at 5739.655 kb on + strand at 5739.655 kb on + strand at 5739.656 kb on - strand at 5739.722 kb on - strand at 5739.722 kb on - strand at 5739.722 kb on - strand at 5739.732 kb on + strand at 5739.732 kb on + strand at 5739.755 kb on - strand at 5739.756 kb on + strand at 5739.757 kb on - strand at 5739.757 kb on - strand at 5739.757 kb on - strand at 5739.790 kb on + strand at 5739.790 kb on + strand at 5739.790 kb on + strand at 5739.790 kb on + strand at 5739.936 kb on + strand at 5739.962 kb on + strand at 5739.975 kb on + strand at 5739.975 kb on + strand at 5739.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction sodium carbonate 0.0125 M remove 5,737,246 + Pf6N2E2_4971 0.86 -0.4 5,737,247 - Pf6N2E2_4971 0.86 +1.3 5,737,247 - Pf6N2E2_4971 0.86 +0.1 5,737,268 + Pf6N2E2_4971 0.89 -0.1 5,737,268 + Pf6N2E2_4971 0.89 +1.0 5,737,269 - Pf6N2E2_4971 0.89 +0.7 5,737,310 - -0.4 5,737,387 + +0.1 5,737,407 - -0.2 5,737,408 + +0.1 5,737,408 + +0.5 5,737,409 - -0.5 5,737,409 - -1.7 5,737,409 - +0.9 5,737,409 - +0.4 5,737,495 + +0.4 5,737,502 + -0.7 5,738,139 - +0.5 5,738,139 - +0.2 5,738,139 - +0.4 5,738,145 - +0.3 5,738,145 - +0.5 5,738,178 - -0.7 5,738,186 + +0.1 5,738,186 + -0.4 5,738,187 - -0.3 5,738,187 - -0.0 5,738,187 - +0.8 5,738,187 - -1.0 5,738,187 - -1.4 5,738,187 - +0.4 5,738,190 - -0.1 5,738,190 - +1.0 5,738,190 - -1.9 5,738,190 - +0.3 5,738,190 - -2.0 5,738,190 - +0.0 5,738,202 - +0.4 5,738,256 - +0.2 5,738,276 + Pf6N2E2_4973 0.11 -0.1 5,738,276 + Pf6N2E2_4973 0.11 -0.4 5,738,277 - Pf6N2E2_4973 0.11 +0.1 5,738,277 - Pf6N2E2_4973 0.11 -1.0 5,738,277 - Pf6N2E2_4973 0.11 -0.8 5,738,277 - Pf6N2E2_4973 0.11 -0.3 5,738,325 - Pf6N2E2_4973 0.17 -1.7 5,738,325 - Pf6N2E2_4973 0.17 +0.1 5,738,325 - Pf6N2E2_4973 0.17 -0.5 5,738,325 - Pf6N2E2_4973 0.17 +0.6 5,738,370 - Pf6N2E2_4973 0.23 +0.1 5,738,552 + Pf6N2E2_4973 0.45 -1.1 5,738,552 + Pf6N2E2_4973 0.45 -2.3 5,738,729 + Pf6N2E2_4973 0.67 -1.1 5,739,137 + Pf6N2E2_4974 0.21 -0.5 5,739,137 + Pf6N2E2_4974 0.21 -1.3 5,739,138 - Pf6N2E2_4974 0.21 -0.7 5,739,207 - Pf6N2E2_4974 0.31 -0.2 5,739,207 - Pf6N2E2_4974 0.31 +0.5 5,739,207 - Pf6N2E2_4974 0.31 -0.0 5,739,207 - Pf6N2E2_4974 0.31 +0.7 5,739,302 + Pf6N2E2_4974 0.46 -0.1 5,739,302 + Pf6N2E2_4974 0.46 -0.1 5,739,326 + Pf6N2E2_4974 0.50 -0.1 5,739,326 + Pf6N2E2_4974 0.50 -1.5 5,739,395 + Pf6N2E2_4974 0.60 -1.1 5,739,396 - Pf6N2E2_4974 0.60 +0.8 5,739,396 - Pf6N2E2_4974 0.60 +0.1 5,739,396 - Pf6N2E2_4974 0.60 +0.7 5,739,396 - Pf6N2E2_4974 0.60 -0.1 5,739,570 - Pf6N2E2_4974 0.87 -1.2 5,739,584 + Pf6N2E2_4974 0.90 -0.4 5,739,608 + -0.7 5,739,608 + +0.3 5,739,608 + -0.8 5,739,655 + -0.2 5,739,655 + -1.6 5,739,655 + -0.0 5,739,655 + -0.0 5,739,655 + -0.1 5,739,655 + +0.1 5,739,655 + -0.0 5,739,656 - +0.6 5,739,722 - +1.5 5,739,722 - +0.8 5,739,722 - -1.1 5,739,732 + -0.6 5,739,732 + -1.1 5,739,755 - -0.8 5,739,756 + +0.6 5,739,757 - +0.2 5,739,757 - +0.7 5,739,757 - +0.9 5,739,790 + +0.4 5,739,790 + -1.5 5,739,790 + +0.7 5,739,790 + +1.0 5,739,936 + -0.2 5,739,962 + -0.3 5,739,975 + +0.2 5,739,975 + -0.1 5,739,976 - +0.6
Or see this region's nucleotide sequence